Yeast glucan particles activate murine resident macrophages to secrete proinflammatory cytokines via MyD88- and Syk kinase-dependent pathways

Li, Bing; Cramer, Daniel E.; Wagner, Stéphanie; Hansen, Richard; King, Chelsea; Kakar, Shelly; Ding, Chuan‐Fan; Yan, Jun

doi:10.1016/j.clim.2007.05.002

Cited by 64 publications

(40 citation statements)

References 36 publications

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“…Reishi, Shiitake and Maitake [8,9]. They are potentreticuloendothelial-modulating agents whose immunobiological activity is mediated by stimulating proinflammatory cytokine production, in part, by an increase in the number and function of macrophages [10], modulation of immunity, and resistance to microbial challenge [11]. The b-glucans can greatly increase numbers of phagocytes for phagocytosis and induce the specific receptor expression to be presented onto the cell surface [12], such as Dectin-1(b GR) and Toll-like receptors (TLR-2) [12][13][14], which recognize and bind to foreign invaders, such as microbes, viruses, yeasts, parasites, and neoplastic cells.…”

Section: Introductionmentioning

confidence: 99%

Aureobasidium-Derived Soluble Branched (1,3-1,6) β-Glucan (Sophy β-glucan) Enhances Natural Killer Activity in Leishmania amazonensis-Infected Mice

et al. 2009

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Abstract:The b-glucans derived from yeast cell walls have been reported for having many immunomodulatory activities in vivo and in vitro. In this study, Aureobasidium-derived soluble branched (1,3-1,6) b-glucan (Sophy b-glucan) was checked for natural killer (NK) activity and for the production of IFN-g and IL-4 in Leishmania amazonensis infection. The main experiment was performed with a group of female C57BL/6 and BALB/c mice, orally supplemented with 5% of Sophy b-glucan and infected with promastogotes of L. amazonensis (1 × 10 7 ) into the footpad. Increase in the footpad thickness with time was observed in BALB/c mice in spite of the oral Sophy b-glucan supplement, but it was less in C57BL/6 mice. The difference in overall mean footpad thickness between 'infection only' versus 'infection + glucan' groups was statistically significant (P < 0.001). High NK activity in C57BL/6 than BALB/c mice was observed in 'glucan only' group compared to the control group and also in 'infection + glucan' group compared to 'infection only' group. The difference in the NK activity among these groups was significant (P < 0.05). The IFN-g level increased at weeks 7 and 8 post-infection in C57BL/6 mice and was significantly high in 'infection + glucan' group compared to the 'infection only' group (P < 0.05). IL-4 levels did not increase up to detectable levels throughout the study. The results led a conclusion that Sophy b-glucan enhances NK activity and cellular immunity in L. amazonensis-infected mice.

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Section: Introductionmentioning

confidence: 99%

Aureobasidium-Derived Soluble Branched (1,3-1,6) β-Glucan (Sophy β-glucan) Enhances Natural Killer Activity in Leishmania amazonensis-Infected Mice

et al. 2009

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“…Some studies have shown that β-glucan isolated from mushroom (Shen et al, 2007), seaweed (Vetvicka et al, 2007), or Saccharomyces cerevisiae (Li et al, 2007;Yoon et al, 2008) influences the cytotoxic activity of immune cells, but β-glucan is simply showing structure of polysaccharides and contains a variety of molecules. Thus, mushrooms might have their own β-glucan.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory effect of mushrooms on cytotoxic activity and cytokine production of intestinal lamina propria leukocytes does not necessarily depend on β-glucan contents

et al. 2011

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We evaluated the effects of seven mushroom extracts (Grifola frondosa, Pholiota nameko, Panellus serotinus, Hypsizygus marmoreus, Pleurotus cornucopiae, Armillaria mellea, and Flammulina velutipes) on cytotoxic activity and cytokine production of lamina propria leukocytes (LPLs) isolated from rat small (S) and large (L) intestinal mucosa. Boiling water extracts from seven species of mushrooms showed no direct cytotoxicity against the YAC-1 target cells. However, prominent increases of cytotoxicity were observed in S-and L-LPLs co-cultured with P. serotinus extract. Cytokine production (TNFα, IFNγ, IL-12 p70, and IL-4) of S-and L-LPLs was stimulated in response to P. cornucopiae extract. Mushroom extracts contribute to target cell adhesion and/or cytokine production in the effector cells. The promotion of cytotoxic activity in S-and L-LPLs was not necessarily related to β-glucan content of the mushroom.

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“…LG Antigen presentation cells (APC) which are able to initiate innate immunity, such as macrophages, express specific surface receptors, such as Dectin-1, to recognize 1,3-β-glucan (29,30). These processed then initiated the adaptive immune responses, accompanied by the secretion of pro-inflammatory cytokines (6,30,31). In the present study, macrophages were isolated from mice treated with 1,3-β-glucan, and co-cultured with lymphocytes in vitro.…”

Section: Discussionmentioning

confidence: 99%

1,3-β-Glucan affects the balance of Th1/Th2 cytokines by promoting secretion of anti-inflammatory cytokines in vitro

Chen

Dong

et al. 2013

Molecular Medicine Reports

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Abstract. 1,3-β-glucan is considered a fungal biomarker and exposure to this agent induces lung inflammation. Previous studies have shown that 1,3-β-glucan affects Th1 and Th2 immune responses. Interleukin (IL)-10 and transforming growth factor (TGF)-β, as typical anti-inflammatory cytokines, suppress the Th1 immune response. To investigate the effects of 1,3-β-glucan on the secretion of cytokines in co-cultured mouse macrophages and lymphocytes in vitro, mice were exposed to 1,3-β-glucan or phosphate-buffered saline (PBS) by intratracheal instillation. Following extraction and co-culture of macrophages and lymphocytes, which were treated with or without 1,3-β-glucan in vitro, enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of cytokines and real-time reverse transcription (RT)-polymerase chain reaction (PCR) was used to investigate the mRNA expression of forkhead box p3 (Foxp3) in the cells. We showed that 1,3-β-glucan exposure in vitro decreased the secretion of Th1 cytokines and increased the secretion of Th2 cytokines in the culture media. Furthermore, 1,3-β-glucan exposure in vitro increased the secretion of IL-10 and TGF-β in the culture media. According to these results, 1,3-β-glucan exposure in vitro is suggested to promote the secretion of anti-inflammatory cytokines, which may lead to a decrease in the levels of Th1 cytokines and an increase in the levels of Th2 cytokines. 1,3-β-Glucan is suggested to induce regulatory lymphocytes, which partly contributes to an increased secretion of anti-inflammatory cytokines in co-cultured mouse macrophages and lymphocytes in vitro.

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Yeast glucan particles activate murine resident macrophages to secrete proinflammatory cytokines via MyD88- and Syk kinase-dependent pathways

Cited by 64 publications

References 36 publications

Aureobasidium-Derived Soluble Branched (1,3-1,6) β-Glucan (Sophy β-glucan) Enhances Natural Killer Activity in Leishmania amazonensis-Infected Mice

Aureobasidium-Derived Soluble Branched (1,3-1,6) β-Glucan (Sophy β-glucan) Enhances Natural Killer Activity in Leishmania amazonensis-Infected Mice

Immunomodulatory effect of mushrooms on cytotoxic activity and cytokine production of intestinal lamina propria leukocytes does not necessarily depend on β-glucan contents

1,3-β-Glucan affects the balance of Th1/Th2 cytokines by promoting secretion of anti-inflammatory cytokines in vitro

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