Vitamin D increases expression of cathelicidin in cultured sebocytes

Lee, Weon Ju; Wuk, Hyun; Sohn, Mi Yeung; Lee, Seok Jong; Kim, Do Won

doi:10.1007/s00403-012-1255-z

Cited by 24 publications

(19 citation statements)

References 29 publications

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“…[13] In addition, vitamin D has antimicrobial effects by inducing antimicrobial peptides such as LL-37 in human sebocytes. [26] These previous reports support the theory that vitamin D has an immune regulatory function in sebocytes, which supports the possible anti-inflammatory effects of vitamin D in acne patients.…”

Section: Discussionsupporting

confidence: 67%

Comparison of Vitamin D Levels in Patients with and without Acne: A Case-Control Study Combined with a Randomized Controlled Trial

Lim

Lee

et al. 2016

PLoS ONE

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BackgroundVitamin D plays an important role in the immune system, and its deficiency has been implicated in various skin diseases, including atopic dermatitis and psoriasis. Acne is a common inflammatory skin disease; however, the association with vitamin D remains unclear.ObjectivesWe evaluated vitamin D levels in patients with acne to determine the effect of vitamin D supplementation.MethodsThis study included 80 patients with acne and 80 healthy controls. Serum 25-hydroxyvitamin D (25(OH)D) levels were measured, and demographic data were collected. Vitamin D-deficient patients were treated with oral cholecalciferol at 1000 IU/day for 2 months.ResultsDeficiency in 25(OH)D was detected in 48.8% of patients with acne, but in only 22.5% of the healthy controls. The level of 25(OH)D was inversely associated with the severity of acne, and there was a significant negative correlation with inflammatory lesions. In a subsequent trial, improvement in inflammatory lesions was noted after supplementation with vitamin D in 39 acne patients with 25(OH)D deficiency.LimitationsLimitations of the study include the small number of patients in the supplementation study and the natural fluctuation of acne.ConclusionsVitamin D deficiency was more frequent in patients with acne, and serum 25(OH)D levels were inversely correlated with acne severity, especially in patients with inflammatory lesions.

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Section: Discussionsupporting

confidence: 67%

Comparison of Vitamin D Levels in Patients with and without Acne: A Case-Control Study Combined with a Randomized Controlled Trial

Lim

Lee

et al. 2016

PLoS ONE

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“…Summarized data are presented as means ± SEM of three to four observations in each group. *P < 0.05, ***P < 0.001 vs. controls (ctrl) through the VDR complex in HaCaT cells (Liu et al 2006;Lee et al 2012). Although we have observed a small reduction in the VDR protein level of HaCaT cells in response to treatment with 1,25D3, the VDR protein expression level is probably still sufficient to mediate the 1,25D3-induced stimulation of CAMP expression.…”

Section: Discussionmentioning

confidence: 97%

Vitamin D-induced up-regulation of human keratinocyte cathelicidin anti-microbial peptide expression involves retinoid X receptor α

et al. 2016

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The biologically active form of vitamin D, 1α,25-dihydroxyvitamin D3 (1,25D3), has been reported to positively regulate the human cathelicidin anti-microbial peptide (CAMP) gene coding for LL-37, but the mechanisms are not completely understood. We have determined the expression of CAMP, vitamin D receptor (VDR), and the retinoid X receptor (RXR) isoforms in human skin and gingival tissue biopsies and investigated the signaling pathways involved in 1,25D3-induced upregulation of CAMP. Human skin and gingival biopsies exhibited few VDR-immunoreactive cells within the stratum basale, whereas rat colon enterocytes (positive control) possessed abundant VDR immunoreactivity. Nuclear VDR immunoreactivity was demonstrated in human skin keratinocytes (HaCaT cells). Gene analysis revealed that human skin biopsies expressed higher levels of both CAMP and RXRα mRNA than human gingival biopsies, whereas VDR and RXRβ transcript levels were similar in skin and gingiva. In HaCaT cells, treatment with 1,25D3 (5 nM and 1 μM) for 4 and 24 h up-regulated CAMP mRNA several fold, and treatment with 1,25D3 for 24 h increased protein expression of the pro-form of LL-37 (hCAP-18) by about 13 times. The 1,25D3-evoked stimulation of HaCaT CAMP expression was associated with attenuated VDR mRNA and protein expression. Treatment with RXRα short interfering RNA reversed the 1,25D3-induced CAMP expression in HaCaT cells, showing that RXRα is involved in the up-regulation of CAMP by 1,25D3. We conclude that the 1,25D3-evoked stimulation of CAMP expression in human skin keratinocytes is dependent on RXRα but is not associated with the up-regulation of VDR expression.

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“…One of the possible ways it is done is through enhanced CYP27B1 expression, subsequent to skin insult, which increases the local synthesis of active vitamin D. Schauber et al [29] have shown that following skin injury, TLR-2 is increased which in turn increases the level of cathelicidin through a vitamin D-dependent mechanism. Similarly, many studies have shown an increased expression of hCAP18/LL-37 and defensin after 1,25(OH) 2 D 3 treatment in keratinocytes and sebocytes [30,31,32,33]. Cathelicidin and β- defensin are direct transcriptional targets of vitamin D, with cathelicidin being induced by binding of the 1,25(OH) 2 D-VDR complex to the VDRE in the promoter region of the gene; however, β-defensin requires nuclear factor κB along with the 1,25(OH) 2 D-VDR complex for its transcription [34].…”

Section: Role Of Vitamin D In Skin Physiologymentioning

confidence: 99%

Vitamin D and the Pathophysiology of Inflammatory Skin Diseases

Umar

Sastry

Ali

et al. 2018

Skin Pharmacol Physiol

143

135

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Background: Vitamin D is a secosteroid, which was initially known for its skeletal role; however, in recent years, its functions in different organs have been increasingly recognized. In this review, we will provide an overview of vitamin D functions in the skin physiology with specific focus on its role in certain inflammatory skin conditions such as psoriasis and atopic dermatitis. Methods: A comprehensive literature search was carried out in PubMed and Google Scholar databases using keywords like “vitamin D,” “skin,” “atopic dermatitis,” and “psoriasis.” Only articles published in English and related to the study topic were included in this review. Results: Vitamin D is integrally connected to the skin for its synthesis, metabolism, and activity. It regulates many physiological processes in the skin ranging from cellular proliferation, differentiation, and apoptosis to barrier maintenance and immune functions. Vitamin D deficiency is associated with the risk of psoriasis and atopic dermatitis, and several clinical/observational studies have suggested the beneficial effect of vitamin D in the therapy of these 2 inflammatory skin disorders. Conclusions: Vitamin D exerts a pleiotropic effect in the skin and could be an important therapeutic option for psoriasis and atopic dermatitis.

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Vitamin D increases expression of cathelicidin in cultured sebocytes

Cited by 24 publications

References 29 publications

Comparison of Vitamin D Levels in Patients with and without Acne: A Case-Control Study Combined with a Randomized Controlled Trial

Comparison of Vitamin D Levels in Patients with and without Acne: A Case-Control Study Combined with a Randomized Controlled Trial

Vitamin D-induced up-regulation of human keratinocyte cathelicidin anti-microbial peptide expression involves retinoid X receptor α

Vitamin D and the Pathophysiology of Inflammatory Skin Diseases

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