Vitamin C-E ve Monosodyum Glutamata Bağlı Ovaryan Toksisite

Abstract

Purpose: Our study aimed to investigate the combinatorial effect of vitamin C and E on oxidative stress, the number of folicles, and hormonal level in female rats orally exposed to monosodium glutamate (MSG). Materials and Methods:Female, twelve weeks old Wistar rats ingested with of MSG at dose 140 mg/200 gram body weight (bw) with or without combination of vitamin C and E. Twenty five rats were divided into five group (n = 5 each), control group, MSG-treatment group, MSG-treatment + 0.2 mg/g bw vitamin C + 0.04 IU/g bw vitamin E (MSG + CE1), MSG-treatment + 0.4 mg/g bw vitamin C + 0.04 IU/g bw vitamin E (MSG + CE2), and MSG-treatment + 0.6 mg/g bw vitamin C + 0.04 IU/g bw vitamin E (MSG + CE1). Analysis of malondialdehyde (MDA) level was done colorimetrically. Analysis of the number of ovarian follicles was done histopathologically with hematoxylin eosin staining. Analysis of 17β-estradiol and follicle stimulating hormone (FSH) levels were done by ELISA technique. Results: There were significantly (P < 0.05) increased ovarium MDA levels and atresia follicle number in groups exposed to MSG compared to non-exposure group. The level of FSH, 17β-estradiol, the number of primary, secondary, de Graaf follicles were significantly lower in MSG-treatment group compared to control group (P < 0.05). The administration of combined vitamin C and E (second and third dose) significantly (P < 0.05) decreased the MDA levels and the number of atresia follicle compared to the MSG-exposed groups, to reach level in control group (P > 0.05). MSG + CE2 and MSG + CE3 significantly increased FSH level, number of primary follicles, compared to MSG-treatment group (P < 0.05), to reach similar level in control group (P > 0.05). All dose of combined vitamin C and E significantly increased 17β-estradiol level and the number of secondary and de Graaf follicles compared to MSG-treatment group (P < 0.05), to reach significantly higher level compared to control group (P < 0.05). Conclusion:The present data suggest that combination of vitamin C and E as in this study inhibited ovarium toxicity caused by MSG treatment. Keywords: glutamate; antioxidant; folliculogenesis; hormone; lipid peroxidation Sadece MSG verilmiş grup ile karşılaştırıldıklarında; C ve E vitaminlerinin tüm dozlarının, 17β-estradiol düzeyi ile sekonder ve graff folikül sayılarını anlamlı olarak arttırdıkları tespit edilmiştir (P < 0.05). C ve E vitaminlerinin verildiği gruplarda, 17β-estradiol düzeyi ile sekonder ve graffolikül sayıları, kontrol grubunun üzerindeki düzeylere ulaşmıştır (P < 0.05).Sonuç: Bulgularımız C ve E vitamin kombinesinin MSG'ya maruz kalmaya bağlı olarak gelişen ovaryum toksisitesini inhibe ettiği sonucunu ortaya koymuştur.

Effect of Monosodium Glutamate on Some Vitamins and the Protective role of Vitamin B12 in Adult Female Rats

“…C and E protected MSG-induced ovarian damage [14]. On the other hand, Group V's results showed an increase, which was ascribed to the vitamins' synergy, which has been linked to a number of metabolic processes.…”

Investigation of the Effects of Monosodium Glutamate on the Embryonic Development of the Eye in Chickens

“…These studies revealed that MSG causes cytotoxicity [ 12 , 13 ] and oxidative damage in the liver, kidney, and various tissues [ 14 , 15 , 16 , 17 , 18 ] and increased the risk of certain cancers [ 16 ]. MSG may adversely affect immune system organs [ 13 , 18 , 19 ], the testes [ 20 , 21 , 22 ], ovarium [ 23 , 24 , 25 , 26 , 27 ], pancreas [ 28 ], spleen [ 29 , 30 , 31 ], liver [ 15 , 32 , 33 ], and cardiac tissue [ 2 ]. It has been reported that the incidence of certain diseases, such as obesity [ 34 , 35 ], diabetes, and Alzheimer’s is increased in people who consume foods with added MSG [ 36 , 37 ].…”