Visualization of reactive oxygen species formation by phagocytizing macrophages

Peskin, Alexander V.; Khramtsov, Andrei; Ia, Morozov; Zemskov, V. M.; Zbarsky, I. B.

doi:10.1016/0014-4827(84)90372-0

Cited by 9 publications

(5 citation statements)

References 8 publications

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“…Our results were also in agreement with Peskin's results [23], the reaction products of TNBT had an adequate electron-density, but the blob that Briggs [5] indicated was also observed. Furthermore, the localization of reaction products was limited to the phagosomal membrane, but didn't show the reaction deposits on the whole surface of PMN.…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

Ultracytochemistry for Detection of O2- in Polymorphonuclear Leukocytes Using the Tetrazolium Method: Comparison of Various Tetrazolium Salts.

Ueda¹,

Ishikawa²,

Takekoshi

1999

Acta Histochem. Cytochem

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Section: Discussionsupporting

confidence: 92%

“…Furthermore, Peskin et al [23] described that TNBT had adequate electron-dense reaction products in stimulated macrophages. These differences are considered to be due to the preparation of the PMN and the consistency of the incubation medium.…”

Section: Discussionmentioning

confidence: 99%

Ultracytochemistry for Detection of O2- in Polymorphonuclear Leukocytes Using the Tetrazolium Method: Comparison of Various Tetrazolium Salts.

Ueda¹,

Ishikawa²,

Takekoshi

1999

Acta Histochem. Cytochem

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“…For instance, using these methods, protein sulfenic acid modifications, oxidative cysteine modifications and unrelated sulfinic or sulfonic acid modifications can be directly detected, which constitute the main regulatory target of ROS [ 50 , 51 ]. Early versions of these technologies relied upon alterations in changes in electron density or enzymatic -based colorimetric changes, meaning visualization was limited to fixed and static detection methods [ 52 ]. Since then, fluorescent probes for ROS-detection have been developed to track the dynamics of specific ROS in real time.…”

Section: Methods For Quantitative Ros Detectionmentioning

confidence: 99%

The role of cellular reactive oxygen species in cancer chemotherapy

Yang

Villani

Wang

et al. 2018

J Exp Clin Cancer Res

599

425

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Most chemotherapeutics elevate intracellular levels of reactive oxygen species (ROS), and many can alter redox-homeostasis of cancer cells. It is widely accepted that the anticancer effect of these chemotherapeutics is due to the induction of oxidative stress and ROS-mediated cell injury in cancer. However, various new therapeutic approaches targeting intracellular ROS levels have yielded mixed results. Since it is impossible to quantitatively detect dynamic ROS levels in tumors during and after chemotherapy in clinical settings, it is of increasing interest to apply mathematical modeling techniques to predict ROS levels for understanding complex tumor biology during chemotherapy. This review outlines the current understanding of the role of ROS in cancer cells during carcinogenesis and during chemotherapy, provides a critical analysis of the methods used for quantitative ROS detection and discusses the application of mathematical modeling in predicting treatment responses. Finally, we provide insights on and perspectives for future development of effective therapeutic ROS-inducing anticancer agents or antioxidants for cancer treatment.

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“…ROS are important mediators of cell signaling (called redox signaling) [2] and are indispensable for the immune defense in macrophages. The enzyme NADPH oxidase, for example, generates superoxide from molecular oxygen, which is then used to kill bacteria in the respiratory burst reaction within the cell [3]. Nonetheless, high levels of ROS contribute to cell toxicity [4], since they also damage host DNA/RNA [5], oxidize proteins [6] or cause lipid peroxidation [7].…”

Section: Introductionmentioning

confidence: 99%

Disease Progression Mediated by Egr-1 Associated Signaling in Response to Oxidative Stress

Pagel

Deindl

2012

IJMS

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When cellular reducing enzymes fail to shield the cell from increased amounts of reactive oxygen species (ROS), oxidative stress arises. The redox state is misbalanced, DNA and proteins are damaged and cellular transcription networks are activated. This condition can lead to the initiation and/or to the progression of atherosclerosis, tumors or pulmonary hypertension; diseases that are decisively furthered by the presence of oxidizing agents. Redox sensitive genes, like the zinc finger transcription factor early growth response 1 (Egr-1), play a pivotal role in the pathophysiology of these diseases. Apart from inducing apoptosis, signaling partners like the MEK/ERK pathway or the protein kinase C (PKC) can activate salvage programs such as cell proliferation that do not ameliorate, but rather worsen their outcome. Here, we review the currently available data on Egr-1 related signal transduction cascades in response to oxidative stress in the progression of epidemiologically significant diseases. Knowing the molecular pathways behind the pathology will greatly enhance our ability to identify possible targets for the development of new therapeutic strategies.

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Visualization of reactive oxygen species formation by phagocytizing macrophages

Cited by 9 publications

References 8 publications

Ultracytochemistry for Detection of O2- in Polymorphonuclear Leukocytes Using the Tetrazolium Method: Comparison of Various Tetrazolium Salts.

Ultracytochemistry for Detection of O2- in Polymorphonuclear Leukocytes Using the Tetrazolium Method: Comparison of Various Tetrazolium Salts.

The role of cellular reactive oxygen species in cancer chemotherapy

Disease Progression Mediated by Egr-1 Associated Signaling in Response to Oxidative Stress

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