Ultracytochemistry for Detection of O2- in Polymorphonuclear Leukocytes Using the Tetrazolium Method: Comparison of Various Tetrazolium Salts.

Ueda, Tadashi; Ishikawa, Yoshimaro; Takekoshi, Noboru

doi:10.1267/ahc.32.351

Cited by 4 publications

(3 citation statements)

References 26 publications

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“…With the addition of NBT, the system turned blue immediately implying that NBT was degraded by ·O 2 − . [ 27 ] UV absorption intensity of the mixtures at 530 nm was monitored each 10 min (Figure 6). It was found the absorption intensity increased rapidly at 10–40 min.…”

Section: Resultsmentioning

confidence: 99%

A novel, green strategy based on bicarbonate activated hydrogen peroxide system for triazine hindered amines nitroxide radicalization for halogen‐free flame retardants

Wang

Yan

et al. 2022

Vinyl Additive Technology

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The triazine hindered amine (THA) nitroxide radicals are important to the preparation of halogen-free flame retardants, but the traditional methods using peracetic acid was limited in further sustainable development in industry. In this work, the bicarbonate-activated hydrogen peroxide system with ethyl acetate as the solvent (BAP-EA system) was firstly employed in TH-7 nitroxide radicalization, which exhibited excellent performance with 99% yield of TH-7-(NOÁ) 2 at 30 C. To further explore the mechanism, the bicarbonate transformation, the generation of reactive oxygen species (ROS), and the effect of ROS were explored by LC-MS, 13 C-NMR, EPR, et al. The results indicated that HCO 4À was generated in BAP system, and then further produced ROS ( 1 O 2 , ÁO 2 À and ÁOH) to participate in the radicalization. ROS also exhibits synergistic effects in BAP-EA system, and further enhanced the reactivity of ROS in TH-7 nitroxide radicalization. Moreover, in the flame retardancy test, NOR 116 synthesized in BAP-EA system displayed excellent flame retardant synergy with aluminum hypophosphite (AH) in polypropylene (PP). This work provides a novel insight in green process of THA nitroxide radicalization for halogen-free flame retardants.

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Section: Resultsmentioning

confidence: 99%

A novel, green strategy based on bicarbonate activated hydrogen peroxide system for triazine hindered amines nitroxide radicalization for halogen‐free flame retardants

Wang

Yan

et al. 2022

Vinyl Additive Technology

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show abstract

“…121 (2001) ics Industry Co., Japan), which was controlled accurately by monitoring the amount of electricity. The concentration of superoxide in DMSO solution amounted to 0.14 mM, which was obtained from nitro blue tetrazolium (NBT) test 14) by measuring the absorption at 520 nm. The generated O -2 solution was used within 30 minutes.…”

Section: Methodsmentioning

confidence: 99%

Superoxide Scavenging Activities of Sixty Chinese Medicines Determined by an ESR Spin-Trapping Method Using Electrogenerated Superoxide.

et al. 2001

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Superoxide-scavenging activities of 60 kinds of Chinese herbal medicines were determined accurately by an electron spin resonance (ESR) spin-trapping technique using 5,5-dimethyl-1-pyrroline 1-oxide (DMPO) as a spin-trapping reagent. As a source of superoxide in this method, superoxide generated by one-electron reduction of the oxygen molecule in dimethyl sulfoxide solution was used. As a result of these studies, very powerful scavenging activity was found in Chinese medicines for in‰ammation, diseases of blood circulation and for tumors.

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“…The nitroblue tetrazolium (NBT) test was used to confirm differentiation (Jarstrand and Akerlund, 1994;Ueda et al, 1999). Briefly, media was removed, and the cells were resuspended in a saturated NBT solution containing 100 ng/mL phorbol myristate acetate.…”

Section: Cell Culture and Isolation Of Peripheral Blood Leukocytesmentioning

confidence: 99%

Variants of the 5′-untranslated region of human NCF2: Expression and translational efficiency

Gauss

Bunger

Crawford

et al. 2006

Gene

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Ultracytochemistry for Detection of O2- in Polymorphonuclear Leukocytes Using the Tetrazolium Method: Comparison of Various Tetrazolium Salts.

Cited by 4 publications

References 26 publications

A novel, green strategy based on bicarbonate activated hydrogen peroxide system for triazine hindered amines nitroxide radicalization for halogen‐free flame retardants

A novel, green strategy based on bicarbonate activated hydrogen peroxide system for triazine hindered amines nitroxide radicalization for halogen‐free flame retardants

Superoxide Scavenging Activities of Sixty Chinese Medicines Determined by an ESR Spin-Trapping Method Using Electrogenerated Superoxide.

Variants of the 5′-untranslated region of human NCF2: Expression and translational efficiency

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