Superoxide Scavenging Activities of Sixty Chinese Medicines Determined by an ESR Spin-Trapping Method Using Electrogenerated Superoxide.

Abstract

Superoxide-scavenging activities of 60 kinds of Chinese herbal medicines were determined accurately by an electron spin resonance (ESR) spin-trapping technique using 5,5-dimethyl-1-pyrroline 1-oxide (DMPO) as a spin-trapping reagent. As a source of superoxide in this method, superoxide generated by one-electron reduction of the oxygen molecule in dimethyl sulfoxide solution was used. As a result of these studies, very powerful scavenging activity was found in Chinese medicines for in‰ammation, diseases of blood circulation and for tumors.

Leonurine Protects Middle Cerebral Artery Occluded Rats Through Antioxidant Effect and Regulation of Mitochondrial Function

“…Herba Leonuri, also named Chinese Motherwort, was reported to have antioxidant properties by its strong superoxide-scavenging ability measured by an electron spin resonance spin trapping technique in vivo. 6 Recently, raw Herba Leonuri was scientifically purified and contains mainly stachydrine (C 7 H 13 NO 2 ), quercetin (C 15 H 10 O 7 ), Kaempferol (C 15 H 10 O 6 ), Leonurine (C 14 H 21 N 3 O 5 ), and apigenin (C 15 H 10 O 5 ). Our previous study showed that purified Herba Leonuri preserves antioxidant properties and does have a cardioprotective effect on ischemic myocardium through an antioxidant effect.…”

The Ethical Kampo Formulation Sho-Seiryu-To (TJ-19) Prevents Bleomycin-Induced Pulmonary Fibrosis in Rats

“…This result suggests that the preventive effects of TJ-19 on BLM-induced pulmonary fibrosis, at least in part, can be ascribed to antioxidative potential. The 3D-HPLC data indicated that TJ-19 contains anti-oxidative constituents including catechin, 6-gingerol, and glycyrrhizin, [14][15][16][17] further supporting the above suggestion. However, in addition to ROS, other inflammatory mediators and mechanisms are involved in the development of BLM-induced pulmonary fibrosis, and these may also affect the inhibitory effects of TJ-19 against BLM-induced pulmonary fibrosis.…”

Bofutsushosan, a Traditional Chinese Formulation, Prevents Intimal Thickening and Vascular Smooth Muscle Cell Proliferation Induced by Balloon Endothelial Denudation in Rats

“…23) Thus BOF containing Rhei Rhizoma 24) and Scutellariae Radix 25) may have atherosclerosis-preventive effect. The inhibitory actions of a Rhei Rhizoma component combined with BOF on nitric oxide 26) and superoxide anion, 27,28) the free radical-scavenging action of Scutellariae Radix, 29) and the scavenging actions of Angelicae and Paeoniae Radix on the superoxide anion and hydroxyl radical 30) are also considered to be involved in the inhibitory effects of BOF on intimal thickening.…”