Virulence genes detection of Salmonella serovars isolated from pork and slaughterhouse environment in Ahmedabad, Gujarat

Abstract

Aim:The aim was to detect virulence gene associated with the Salmonella serovars isolated from pork and Slaughterhouse environment.Materials and Methods:Salmonella isolates (n=37) used in this study were isolated from 270 pork and slaughter house environmental samples collected from the Ahmedabad Municipal Corporation Slaughter House, Ahmedabad, Gujarat, India. Salmonella serovars were isolated and identified as per BAM USFDA method and serotyped at National Salmonella and Escherichia Centre, Central Research Institute, Kasauli (Himachal Pradesh, India). Polymerase chain reaction technique was used for detection of five genes, namely invA, spvR, spvC, fimA and stn among different serovars of Salmonella.Results:Out of a total of 270 samples, 37 (13.70%) Salmonella were isolated with two serovars, namely Enteritidis and Typhimurium. All Salmonella serovars produced 284 bp invA gene, 84 bp fimA and 260 bp amplicon for enterotoxin (stn) gene whereas 30 isolates possessed 310 bp spvR gene, but no isolate possessed spvC gene.Conclusion:Presence of invA, fimA and stn gene in all isolates shows that they are the specific targets for Salmonella identification and are capable of producing gastroenteric illness to humans, whereas 20 Typhimurium serovars and 10 Enteritidis serovars can able to produce systemic infection.

Genetic diversity of food originated Salmonella isolates

“…Some studies reported the detection of this gene in all tested Salmonella spp. isolates [49][50][51][52][53][54][55][56][57][58]. On the other side, Moussa et al [59] and Maysa and Abd-Elall [60] couldn't find invA gene in all isolates, same as our study.…”

“…None of all 37 Salmonella isolated from pork and slaughterhouse by Chaudhary et al [57] possessed the spvC gene. This variable state suggested that the prevalence of Salmonella virulence plasmids is restricted within the isolates of some defined origin of sources.…”

Characterization of Salmonella Gallinarum isolates from backyard poultry by polymerase chain reaction detection of invasion (invA) and Salmonella plasmid virulence (spvC) genes

“…High prevalence of inv A virulence gene in Salmonella serovars has also been reported by other workers [ 22 , 23 ]. We found inv A gene was 100% similar with other Salmonella serovars.…”

Molecular Detection of InvA, OmpA and Stn Genes in Salmonella Serovars from Broilers in Egypt

“…It has been reported that invA gene is present only in Salmonella species and therefore is used as a golden marker in genetic diagnosis of Salmonella species as previously described (O'Regan et al, 2008). In our study the invA gene was detected with 100% in all examined Salmonella serovars, high prevalence rates of invA virulence gene in Salmonella serovars has also been reported by other workers (Karmi, 2013 andChaudhary et al, 2015). In addition, the result of our study revealed that the stn gene was present in all of the isolates 100%.…”