Molecular Detection of InvA, OmpA and Stn Genes in Salmonella Serovars from Broilers in Egypt

Fekry, Esraa; Eman-abdeen, E; Ammar, Ahmed M.; Hussien, Alaa-edin

doi:10.5455/ajvs.288089

Cited by 9 publications

(9 citation statements)

References 12 publications

(12 reference statements)

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“…The inv A gene for instance affects the host cell by delivery of type III secreted effectors, for mutant phenotype, and is also essential for invasion of epithelial cells (El-Sharkawy et al 2017 ; Marcus et al 2000 ). The inv A gene has been confirmed to be present in Salmonella species only and hence is used in the genetic diagnosis of Salmonella species (Fekry, Ammar & Hussien 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of virulence genes in Salmonella spp. isolated from chicken faeces in Mafikeng, South Africa

Ramatla

Mphuthi

Ramaili

et al. 2020

Journal of the South African Veterinary Association

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Chickens have been implicated in most Salmonella disease outbreaks because they act as carriers of the pathogen in their gut. There are over 2500 serotypes of Salmonella that have been reported worldwide and 2000 of these serovars can be found in chickens. The main objective of this study was to determine the Salmonella serotypes found in poultry farms around Mafikeng district, South Africa. Salmonella was identified according to the guidelines of the International Organization for Standardization (ISO) (ISO 6579:2002) standard techniques. Faecal samples were collected and analysed for Salmonella using conventional cultural methods and polymerase chain reaction targeting the 16S Ribosomal Deoxyribonucleic acid (rDNA) gene for Salmonella identification. Out of 130 presumptive Salmonella isolates determined by urease and triple sugar iron tests, only 46 isolates were identified as Salmonella serotypes of which S . Typhimurium was the most frequent with 18 (39.1%), followed by S . Heidelberg with 9 (19.6%), S. bongori with 7 (15.2%), S . Enteritidis with 6 (13.0%) and both S. Paratyphi B and S . Newport with 3 (6.5%) each. Seven virulence genes including inv A 100%, spy 39%, hil A 9%, mis L 30%, sdf I 13%, orf L 11% and spi C 9% were detected from these Salmonella isolates in this study. The presence of these virulence genes indicates high pathogenicity potential of these isolates which is a serious public health concern because of zoonotic potential of Salmonella .

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Section: Introductionmentioning

confidence: 99%

Molecular detection of virulence genes in Salmonella spp. isolated from chicken faeces in Mafikeng, South Africa

Ramatla

Mphuthi

Ramaili

et al. 2020

Journal of the South African Veterinary Association

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“…Salmonella pathogenicity has been related to many virulence genes existent in the chromosomal Salmonella pathogenicity islands (SPIs) (Nayak et al, 2004). The invA gene is essential for epithelial cells invasion (El-Sharkawy et al, 2017) and has been established to be present in Salmonella species only, hence it is used in the genetic diagnosis of Salmonella species (Fekry et al, 2018). From medical and pharmaceutical points of view invA gene can help in developing specific medicines against salmonella (Almas et al, 2021).…”

mentioning

confidence: 99%

Virulence and Antibiotic Resistance Profiles of Salmonella Isolated from Chicken Ready Meals and Humans in Egypt

Mubarak¹,

Mustafa²,

Abdelazeem³

et al. 2021

AAVS

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S almonellosis remains a major public health issue worldwide with a huge global burden of morbidity and mortality especially in developing countries (Sodagari et al., 2020). It is estimated to cause 93.8 million human infections and 300,000 deaths annually (WHO, 2020) besides causing a major challenge in the global poultry industry. It is well known that human salmonellosis is associated with the consumption of different kinds of food, in particular poultry and poultry products (Favier et al., 2013). Other routes of infection between individuals are represented by the fecal-oral route and contact with infected pets through contamination of food and drink by the hands, thus disease outbreaks can occur (Munck et al., 2020).

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“…Salmonella enterotoxin (stn) is a gene encoding stn protein that causes gastroenteritis leading to nausea, vomiting, abdominal cramps, fever, and diarrhea. stn gene is detected in all Salmonella strains from broilers (Fekry et al 2018). The virulence genes detected in this study were the stn gene, the fimA gene, and the spvR gene.…”

Section: Resultsmentioning

confidence: 57%

Detection of virulence and specific genes of Salmonella sp. indigenous from Jember, Indonesia

et al. 2020

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Abstract. Yulian R, Narulita E, Iqbal M, Rochmiyah D, Suryaningsih I, Ningrum DEAF. 2020. Detection of virulence and specific genes of Salmonella sp. indigenous from Jember, Indonesia. Biodiversitas 21: 2889-2892. The bacterium Salmonella sp. is the most common cause of foodborne infection in humans. It has the ability to invAde the host, Salmonellosis is highly influenced by the combination of chromosome and plasmid in which Salmonella Pathogenisitas Islands (SPIs) are the biggest gene in the chromosome that are responsible to build specific interaction between Salmonella and the host. This research performed detection on virulence and specific genes in the SPIs area using PCR method for two Salmonella spp. bacterial isolates originated from Jember which were KP2 and P21D. Seven pairs of primers including stn, fimA, spvR (virulence genes), invA, ivaB, spvC, and fliC-d (specific genes) were used. The result revealed that P21D bacteria containing 3 virulence genes i.e. stn, fimA, and spvR. Meanwhile, only two virulent genes stn, and spvR were present in KP2 bacteria. For the detection of specific genes, three genes invA, ivaB, and spvC were present while fliC-d genes were not detected. Only two, invA, and ivaB genes were detected in P21D bacteria, while spvC, and fliC-d were not detected.

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Molecular Detection of InvA, OmpA and Stn Genes in Salmonella Serovars from Broilers in Egypt

Cited by 9 publications

References 12 publications

Molecular detection of virulence genes in Salmonella spp. isolated from chicken faeces in Mafikeng, South Africa

Molecular detection of virulence genes in Salmonella spp. isolated from chicken faeces in Mafikeng, South Africa

Virulence and Antibiotic Resistance Profiles of Salmonella Isolated from Chicken Ready Meals and Humans in Egypt

Detection of virulence and specific genes of Salmonella sp. indigenous from Jember, Indonesia

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