In this study, we develop a Multi-constellation Global Navigation Satellite System (GNSS) Receiver Differential Code Bias (MGR-DCB) model. The model estimates the receiver DCBs for the Global Positioning System (GPS), BeiDou and Galileo signals from the ionosphere-corrected geometry-free linear combinations of the code observations. In order to account for the ionospheric delay, a Regional Ionospheric Model (RIM) over Europe is developed. GPS observations from 60 International GNSS Servoce (IGS) and EUREF reference stations are processed in the Bernese-5·2 Precise Point Positioning (PPP) module to estimate the Vertical Total Electron Content (VTEC). The RIM has spatial and temporal resolutions of 2. Differential Code Bias.
Assessment Study of Using Online (CSRS) GPS-PPP Service for Mapping Applications in EgyptMany applications in navigation, land surveying, land title definitions and mapping have been made simpler and more precise due to accessibility of Global Positioning System (GPS) data, and thus the demand for using advanced GPS techniques in surveying applications has become essential. The differential technique was the only source of accurate positioning for many years, and remained in use despite of its cost. The precise point positioning (PPP) technique is a viable alternative to the differential positioning method in which a user with a single receiver can attain positioning accuracy at the centimeter or decimeter scale. In recent years, many organizations introduced online (GPS-PPP) processing services capable of determining accurate geocentric positions using GPS observations. These services provide the user with receiver coordinates in free and unlimited access formats via the internet. This paper investigates the accuracy of the Canadian Spatial Reference System (CSRS) Precise Point Positioning (PPP) (CSRS-PPP) service supervised by the Geodetic Survey Division (GSD), Canada. Single frequency static GPS observations have been collected at three points covering time spans of 60, 90 and 120 minutes. These three observed sites form baselines of 1.6, 7, and 10 km, respectively. In order to assess the CSRS-PPP accuracy, the discrepancies between the CSRS-PPP estimates and the regular differential GPS solutions were computed. The obtained results illustrate that the PPP produces a horizontal error at the scale of a few decimeters; this is accurate enough to serve many mapping applications in developing countries with a savings in both cost and experienced labor.
Recently, the International Global Navigation Satellite System (GNSS) Service (IGS) has launched the Real-Time Service (IGS-RTS). The RTS products enable real-time precise positioning applications. For single-frequency Real-Time Precise Point Positioning (RT-PPP), ionospheric delay mitigation is a major challenge. To overcome this challenge, we developed a Real-Time Regional Ionospheric Model (RT-RIM) over Europe using the RTS satellite orbits and clock products. The model has spatial and temporal resolution of 1°× 1°and 15 minutes, respectively. Global Positioning System (GPS) observations from 60 IGS and EUREF reference stations are processed using the Bernese 5·2 PPP module in order to extract the Real-Time Vertical Electron Content (RT-VTEC). The PPP convergence time and positioning accuracy using the RTS products is estimated and compared with dual frequency PPP and single-frequency PPP obtained through the combined rapid IGS Global Ionospheric Maps (IGS-GIM) over three consecutive days under high solar activity and one of them under active geomagnetic activity. The results show that the proposed model improves PPP accuracy and convergence time under the mid-latitude region about 40%, 55% and 40% for the horizontal, height and three-dimensional (3D) components respectively in comparison with the IGS-GIM.
K E Y WO R D S1. IGS-RTS Products.2. PPP. 3. Ionospheric model.
In this study, we propose a regional ionospheric model (RIM) based on both of the GPS-only and the combined GPS/BeiDou observations for single-frequency precise point positioning (SF-PPP) users in Europe. GPS/BeiDou observations from 16 reference stations are processed in the zero-difference mode. A least-squares algorithm is developed to determine the vertical total electron content (VTEC) bi-linear function parameters for a 15-minute time interval. The Kriging interpolation method is used to estimate the VTEC values at a 1 ° × 1 ° grid. The resulting RIMs are validated for PPP applications using GNSS observations from another set of stations. The SF-PPP accuracy and convergence time obtained through the proposed RIMs are computed and compared with those obtained through the international GNSS service global ionospheric maps (IGS-GIM). The results show that the RIMs speed up the convergence time and enhance the overall positioning accuracy in comparison with the IGS-GIM model, particularly the combined GPS/BeiDou-based model.
Brain samples were collected from 33 animals of different species, including buffalo, cattle, dog, donkey, fox and wolf, that had been suspected to be infected by rabies virus (RABV) in different geographical regions of Aswan and Luxor governorates in Egypt. The samples were submitted for histopathological examination and the presence of the nucleic acid and antigens of RABV was tested by RT-PCR and indirect fluorescent antibody technique (IFAT), respectively. Sixteen samples were found positive by all the three examinations. Three samples were selected for further study from animals in which the highest virus loads were detected. The partial sequence of the RABV N gene was determined and analysed from the samples of a buffalo, a cow and a donkey. The viruses in the samples were found to share 95–98% and 95–97% nucleotide and amino acid sequence identities, respectively. In comparison to reference sequences, a few amino acid substitutions occurred in the N protein antigenic sites I and IV in the immunodominant epitopes of the viruses detected in the cow and the donkey but not in the one from the buffalo. The phylogenetic analysis revealed that the RABVs sequenced from the samples belonged to genotype 1, Africa-4 clade, and formed two distinct sub-clades within the Egyptian clade. These findings indicate the circulation of RABV among livestock animals in the southern part of Egypt and raise public health concerns. The amino acid changes detected in this work may contribute to the antigenic diversification of RABVs.
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