Characterization of Salmonella Gallinarum isolates from backyard poultry by polymerase chain reaction detection of invasion (invA) and Salmonella plasmid virulence (spvC) genes

Pal, Susmita; Dey, Samir; Batabyal, K.; Banerjee, Abhiroop; Joardar, Siddhartha Narayan; Samanta, Indranil; Isore, D.P.

doi:10.14202/vetworld.2017.814-817

Cited by 17 publications

(17 citation statements)

References 25 publications

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“…invA gene is a unit of the SPI 1 which encodes a protein in the inner membrane of bacteria which is responsible invasion to the epithelial cells of the host (2). The PCR assay for the detection of invA gene showed a proportion of 74.13% (43/58) which is lesser in comparison with other studies carried out where invA gene was present in all the NTS isolates (10, 26). A study done by Smith et al in Nigeria showed the PCR analysis of the NTS isolates for the invA gene was 96.1% (27).…”

Section: Discussioncontrasting

confidence: 71%

Poultry: A receptacle for non-typhoidal Salmonellae and antimicrobial resistance

Bangera¹,

Umakanth²,

Chowdhury³

et al. 2019

IJM

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Background and Objectives: Non-typhoidal Salmonellosis, a zoonotic infection associated with acute gastroenteritis is caused by non-typhoidal salmonellae (NTS). The study was carried out to determine the prevalence of NTS serovars and their antimicrobial resistance along with the presence of the virulence gene (invA gene) in poultry samples. Materials and Methods: This is a prospective cross-sectional study carried out at the Enteric Diseases Division, Kasturba Medical College, Manipal, South India from January 2016– December 2017. Poultry samples were collected randomly from two local poultry farms in Udupi district and processed following CDC standard protocol. Results: From the 396 poultry meat samples, intestinal contents and faecal samples collected, 58 NTS serovars were isolated showing a prevalence of 14.64%. Salmonella Infantis, 43.1%, 25/58 was the commonest serovar. Resistance to ciprofloxacin 72.41%, ampicillin 32.8%, gentamicin 17.24%, cotrimoxazole 29.31% and amoxicillin-clavulanic acid 6.9% was observed. The invA gene was detected in 43 NTS isolates (74.13%). Conclusion: Poultry sources are recognized as a significant cause for non-typhoidal salmonellosis. Therefore, hygienic measures should be initiated to reduce the contamination of meat and poultry products with virulent strains of Salmonella that are of public health significance.

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Section: Discussioncontrasting

confidence: 71%

Poultry: A receptacle for non-typhoidal Salmonellae and antimicrobial resistance

Bangera¹,

Umakanth²,

Chowdhury³

et al. 2019

IJM

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“…Despite the implementation of various interventions, SE remains a major cause of outbreaks related to consumption of contaminated poultry products (Antunes et al, 2016). In addition to conventional poultry systems, SE has also been isolated from backyard flocks (Kauber et al, 2017;Pal et al, 2017). The U.S. Centers for Disease Control and Prevention (CDC) recently reported multistate Salmonella outbreaks linked with backyard poultry in which 1003 people were infected from 49 states resulting in 175 hospitalizations and 2 deaths (CDC, 2018).…”

Section: Introductionmentioning

confidence: 99%

Sodium Butyrate Reduces Salmonella Enteritidis Infection of Chicken Enterocytes and Expression of Inflammatory Host Genes in vitro

et al. 2020

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Salmonella Enteritidis (SE) is a facultative intracellular pathogen that colonizes the chicken gut leading to contamination of carcasses during processing. A reduction in intestinal colonization by SE could result in reduced carcass contamination thereby reducing the risk of illnesses in humans. Short chain fatty acids such as butyrate are microbial metabolites produced in the gut that exert various beneficial effects. However, its effect on SE colonization is not well known. The present study investigated the effect of sub-inhibitory concentrations (SICs) of sodium butyrate on the adhesion and invasion of SE in primary chicken enterocytes and chicken macrophages. In addition, the effect of sodium butyrate on the expression of SE virulence genes and selected inflammatory genes in chicken macrophages challenged with SE were investigated. Based on the growth curve analysis, the two SICs of sodium butyrate that did not reduce SE growth were 22 and 45 mM, respectively. The SICs of sodium butyrate did not affect the viability and proliferation of chicken enterocytes and macrophage cells. The SICs of sodium butyrate reduced SE adhesion by ∼1.7 and 1.8 Log CFU/mL, respectively. The SE invasion was reduced by ∼2 and 2.93 Log CFU/mL, respectively in chicken enterocytes (P < 0.05). Sodium butyrate did not significantly affect the adhesion of SE to chicken macrophages. However, 45 mM sodium butyrate reduced invasion by ∼1.7 Log CFU/mL as compared to control (P < 0.05). Exposure to sodium butyrate did not change the expression of SE genes associated with motility (flgG, prot6E), invasion (invH), type 3 secretion system (sipB, pipB), survival in macrophages (spvB, mgtC), cell wall and membrane integrity (tatA), efflux pump regulator (mrr1) and global virulence regulation (lrp) (P > 0.05). However, a few genes contributing to type-3 secretion system (ssaV, sipA), adherence (sopB), macrophage survival (sodC) and oxidative stress (rpoS) were upregulated by at least twofold. The expression of inflammatory genes (Il1β, Il8, and Mmp9) that are triggered by SE for host colonization was significantly downregulated (at least 25-fold) by sodium butyrate as compared to SE (P < 0.05). The results suggest that sodium butyrate has an anti-inflammatory potential to reduce SE colonization in chickens.

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“…Os hospedeiros naturais do tifo aviário são as galinhas. Mas outros animais como os perus, aves caipiras e selvagens, angolas, e aves em geral também podem servir como fonte de infecção (Pal et al, 2017). Além disso, trabalhadores de granjas também podem transmitir a doença.…”

Section: Epidemiologiaunclassified

Salmonelose Aviária

Stella

Costa

Ventura

et al. 2021

RSD

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A salmonelose aviária é um termo usado para se referir aos três tipos diferentes de doenças bacterianas causadas pelo gênero Salmonella sp (pulorose, tifo aviário e paratifo aviário) nas aves domésticas. A pulorose tem sua etiologia relacionada à Salmonella pullorum, o tifo aviário à Salmonella gallinarum e o paratifo aviário a todos os demais sorotipos patogênicos entéricos. As três doenças podem acometer aves domésticas de qualquer idade causando sinais clínicos inespecíficos e sistêmicos, com quadros agudos e crônicos que podem ser confundidos com outras bacterioses. Mundialmente disseminadas, essas doenças são responsáveis por causar impactos na economia brasileira e na saúde pública. A transmissão pode ocorrer pela via vertical e horizontal e pelo contato indireto com alimentos, água de beber, vetores e fômites contaminados. O diagnóstico das aves doentes pode ser realizado com base no histórico, sinais clínicos, achados anatomopatólogicos e exames laboratoriais que incluem a identificação dos agentes por meio de isolamento bacteriano, soroaglutinação rápida em placas, soroaglutinação lenta em tubos e reação em cadeia da polimerase (PCR). Como não há tratamento antibiótico que elimine completamente a infecção nas aves as medidas de controle e prevenção são indispensáveis no controle. O presente artigo trata-se de uma revisão de literatura e tem como objetivo conhecer o referencial teórico referente à doença e aos seus agentes, além da possibilidade de esclarecer a importância da prevenção e do controle desse microrganismo, devido ao caráter infectocontagioso e zoonótico da doença, que pode causar inúmeros prejuízos econômicos e impactos negativos para a saúde pública.

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Characterization of Salmonella Gallinarum isolates from backyard poultry by polymerase chain reaction detection of invasion (invA) and Salmonella plasmid virulence (spvC) genes

Cited by 17 publications

References 25 publications

Poultry: A receptacle for non-typhoidal Salmonellae and antimicrobial resistance

Poultry: A receptacle for non-typhoidal Salmonellae and antimicrobial resistance

Sodium Butyrate Reduces Salmonella Enteritidis Infection of Chicken Enterocytes and Expression of Inflammatory Host Genes in vitro

Salmonelose Aviária

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