Virtual crossmatch by identification of donor-specific anti-human leukocyte antigen antibodies by solid-phase immunoassay: A 30-month analysis in living donor kidney transplantation

Predictive value of the Luminex single antigen panel for detecting flow cytometry cross-match positivity

“…A clear goal in this field has been the prediction of cross-match results with data from SPI [23,24]. CDC has been for many years the only technique available for detecting antidonor antibodies.…”

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“…7,9 Therefore, virtual crossmatching has enabled prediction of actual crossmatch results with a high degree of sensitivity and specificity. 10,11,16 The objectives of this study were to determine patient and allograft outcomes after kidney transplant in sensitized recipients when solid-phase single antigen bead assays were used to detect HLA antibodies and stringent HLA epitope analyses with protein sequence alignment were used for virtual crossmatching. The results indicated that, under contemporary main tenance immunosuppression regimens, the use of this advanced HLA antibody detection and HLA epitope analysis provides similar outcomes, regardless of PRA and induction immunosuppression.…”

“…8,9 Therefore, virtual crossmatching has enabled accurate prediction of the actual crossmatch results with a high degree of sensitivity and specificity. 7,8,10,11 Recent studies have demonstrated that rabbit antithymocyte globulin (rATG) induction immunosuppression may confer benefits regarding long-term outcomes in patients who have high immunologic risk. 12,13 However, few data exist concerning factors important in determining outcomes after kidney transplant in patients with and without high sensitization under contemporary maintenance immunosuppression.…”

Role of crossmatch testing when Luminex-SAB is negative in renal transplantation

“…Amajor advantage of the Luminex-SAB is that, in the case of negative results, one could omit CDCXM testing, which reduces cold ischemia time and the need of immunosuppression. False positive results are among the major limitation of the Luminex-SAB; they are due to technical reasons as they do not detect all HLA-directed antibodies but only those against the most common HLA molecules (34,35,36).…”