Predictive value of the Luminex single antigen panel for detecting flow cytometry cross-match positivity

Moreno, Cristina; Burgos, Leire; Pérez-Robles, Carmen; Delgado, José A.; Mata, Juan José; Errasti, P; Martín, Paloma Leticia; Merino, Juana; Sánchez-Ibarrola, A

doi:10.1016/j.humimm.2012.02.022

Cited by 11 publications

(5 citation statements)

References 32 publications

(25 reference statements)

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“…Furthermore, the good correlation we found between the biological effects on HUVECs and the LSA level for HLA class I, as well as the very poor expression of HLA class II (HLA -DR) on HUVECs, led us to the conclusion that the effects described in this paper are mediated exclusively by HLA class I and not by non-HLA antibodies. Moreover, we have published that human alloimmunized sera bind to cells and that a good correlation is obtained between the level of HLA antibodies MFI and FCXM positivity [40]. Thus, our data allow the identification of the antibody levels capable of mediating different biological effects on ECs, adding value to the detection of anti-HLA antibodies by LSA.…”

Section: Human Anti-hla Antibodies Induce the Expression Of P-selectin In Huvecssupporting

confidence: 55%

“…We grew ECs at very low ECGS concentrations to avoid excessive stimulation by growth factors and for better identification of the effects of anti-HLA antibodies. We show that the effects on HUVECs are observed only when the Luminex MFI reaches a particular level defined by the sum of MFI of antibodies directed to the different HLA antigens expressed by the cell under study [40]. The level of anti-HLA antibodies necessary to produce the described biological effects is different for each particular effect.…”

Section: Discussionmentioning

confidence: 85%

“…These data add information on the pathogenic mechanisms of AMR. Moreover, the detection of HLA antibody levels by LSA has been shown to correlate with other parameters and can be used as a substitutive in some special clinical situations, e.g., virtual cross-match [39,40,44].…”

Section: Discussionmentioning

confidence: 99%

“…Normalized Mean Fluorescence Intensity (MFI) values > 1,000 were defined as positive reaction for anti-HLA class I and class II antibodies. When more than one antigen was recognized by a pool of sera, we utilized the MFI summative strategy to assign the antibody level present in the serum in relation to that cell as was proposed by us and others [17,39,40]. Levels of anti-HLA antibodies > 18,000, measured as the sum of MFI, were considered high [37].…”

Section: Isolation and Culture Of Huvecsmentioning

confidence: 99%

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Distinct Levels of Human Anti-HLA Antibodies Induce Different Biological Effects in vitro on Human Umbilical Vein Endothelial Cells

Mata¹,

Moreno²,

Arana³

et al. 2018

J Cell Dev Biol

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Objectives: Antibody-mediated rejection due to anti-donor HLA antibodies is a cause of chronic rejection and poor graft survival. The pathogenic role of these antibodies has been explained by their different effects on endothelial cells. Our objective was to study whether the levels of anti-HLA antibodies (measured by Luminex Single Antigen) in sera from alloimmunized patients correlate with the in vitro effects these antibodies induce on human umbilical vein endothelial cells. Material and methods:We explored the role of anti-HLA antibodies present in human serum on the proliferative potential of human umbilical vein endothelial cells (n = 17) and studied the expression of adhesion molecules (P-selectin) in these cells (n = 13). Results were correlated with the HLA antibody levels measured by Luminex. Results:The sera from alloimmunized patients inhibited endothelial cells proliferation and increased P-selectin expression compared to control sera (p < 0.0001 and p < 0.001, respectively). A relatively high level of anti-HLA antibodies was necessary to mediate the biological effects, with higher levels needed to affect proliferation (mean fluorescence intensity = 23,219) than to induce changes in P-selectin expression (mean fluorescence intensity = 13,170).Conclusions: These data add information on the value of the detection of anti-HLA antibodies by Luminex and could contribute to clarifying some aspects of the pathogenic mechanisms of antibody-mediated rejection.

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Section: Human Anti-hla Antibodies Induce the Expression Of P-selectin In Huvecssupporting

confidence: 55%

Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Isolation and Culture Of Huvecsmentioning

confidence: 99%

See 2 more Smart Citations

Distinct Levels of Human Anti-HLA Antibodies Induce Different Biological Effects in vitro on Human Umbilical Vein Endothelial Cells

Mata¹,

Moreno²,

Arana³

et al. 2018

J Cell Dev Biol

Self Cite

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“…16,114,125,127,133,135,140 Genetic typing of HLA has also been performed using BBMA technology and had good correlation with single-antigen assay flow cytometry cross-match outcomes. 110 In cancer studies, the humoral responses to peptide cancer vaccines have correlated with cancer survival rates using BBMAs developed to measure the responses to specific peptides. 80 Other applications are for the detection of multiple antibodies 14,16,53,60 and hormones.…”

Section: Human Disease Diagnostic Assays As a Preview Of Potential Veterinary Diagnostic Assaysmentioning

confidence: 99%

Opportunities for bead-based multiplex assays in veterinary diagnostic laboratories

et al. 2013

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Abstract. Bead-based multiplex assays (BBMAs) are applicable for high throughput, simultaneous detection of multiple analytes in solution (from several to 50-500 analytes within a single, small sample volume). Currently, few assays are commercially available for veterinary applications, but they are available to identify and measure various cytokines, growth factors and their receptors, inflammatory proteins, kinases and inhibitors, neurobiology proteins, and pathogens and antibodies in human beings, nonhuman primates, and rodent species. In veterinary medicine, various nucleic acid and protein-coupled beads can be used in, or for the development of, antigen and antibody BBMAs, with the advantage that more data can be collected using approximately the same amount of labor as used for other antigen and antibody assays. Veterinary-related BBMAs could be used for detection of pathogens, genotyping, measurement of hormone levels, and in disease surveillance and vaccine assessment. It will be important to evaluate whether BBMAs are "fit for purpose," how costs and efficiencies compare between assays, which assays are published or commercially available for specific veterinary applications, and what procedures are involved in the development of the assays. It is expected that many veterinary-related BBMAs will be published and/or become commercially available in the next few years. The current review summarizes the BBMA technology and some of the currently available BBMAs developed for veterinary settings. Some of the human diagnostic BBMAs are also described, providing an example of possible templates for future development of new veterinary-related BBMAs.

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Calculated panel-reactive antibody predicts outcomes on the heart transplant waiting list

Kransdorf

Kittleson

Patel

et al. 2017

The Journal of Heart and Lung Transplantation

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Predictive value of the Luminex single antigen panel for detecting flow cytometry cross-match positivity

Cited by 11 publications

References 32 publications

Distinct Levels of Human Anti-HLA Antibodies Induce Different Biological Effects in vitro on Human Umbilical Vein Endothelial Cells

Distinct Levels of Human Anti-HLA Antibodies Induce Different Biological Effects in vitro on Human Umbilical Vein Endothelial Cells

Opportunities for bead-based multiplex assays in veterinary diagnostic laboratories

Calculated panel-reactive antibody predicts outcomes on the heart transplant waiting list

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