Cross-sectional study conducted among workers of the Family Health Strategy Montes Claros. Objective: to investigate the report of vaccination against Hepatitis B, verifi cation of immunization and the factors associated with dosages of anti-HBs. Method: we collected blood samples from those reported that they had one or more doses of the vaccine. We evaluated the association of the dosage of anti-HBs with sociodemographic conditions, occupational and behavioral. The associations were verifi ed by Mann Whitney and Kruskal Wallis and correlation Spermann by linear regression using SPSS ® 17.0. Results: among the 761 respondents, 504 (66.1%) were vaccinated, 52.5 % received three doses, 30.4 % verifi ed immunization. Of the 397 evaluated for the determination of anti-Hbs, 16.4% were immune. Conclusion: it was found that longer duration of work was associated with higher levels of antiHBs, while levels of smoking were inversely associated with anti-HBs. These workers need for vaccination campaigns.