Vaccination strategies with oral booster for surubim hybrid (Pseudoplatystoma corruscans × P. reticulatum) against haemorrhagic septicaemia

Abstract

This study evaluated the efficiency of differently prepared vaccines against Aeromonas hydrophila in the hybrid surubim (Pseudoplatystoma corruscans 9 P. reticulatum). Survival and haematoimmunological parameters were compared between the treatments: non-vaccinated fish (C); bacterin-vaccinated fish (B); bacterin plus oral booster vaccinated fish (B+O); bacterin and toxoid-vaccinated fish (B+T) and bacterin, toxoid and oral booster-vaccinated fish (B+T+O). Fourteendays vaccinated fish from B+O and B+T+O were fed with an oral booster for 4 days. After 1 week, the fish were intraperitoneally challenged with 2 9 10 8 CFU mL À1 of A. hydrophila. Fish from the treatment B+T+O showed the lowest cumulative mortality (11.36%) 96 h after challenge, compared with other treatments (22.72-44.04%), and a relative survival of 74%. Serum immunoglobulin in B+T+O fish was higher than in other treatments. All vaccinated fish showed an increased agglutination titre when compared with non-vaccinated fish, both before and after challenge. Fish fed with oral booster showed an increase in phagocytic percentage before and after challenge. It can be inferred that the oral booster vaccination was efficient in reducing mortality in hybrid surubim by enhancing the response against haemorrhagic septicaemia due to A. hydrophila infection.

Probiotic supplementation in diet and vaccination of hybrid surubim (Pseudoplatystoma reticulatum♀ x P. corruscans♂)

“…Thus, 100mL of vaccine was sprayed using a sterile plastic spray bottle for each kilogram of commercial diet or the diet containing probiotic following methodology previously described by (PEREIRA et al, 2015).…”

“…The diet containing vaccine was also provided four times a day (3% of the biomass). The vaccination protocol and sampling was carried out according PEREIRA et al (2015).…”

Haemato-immunological responses and effectiveness of feed-based bivalent vaccine against Streptococcus iniae and Aeromonas hydrophila infections in hybrid red tilapia (Oreochromis mossambicus × O. niloticus)

“…Sirimanapong [49] showed that the number of lymphocyte was also signi cantly higher in the vaccinated sh by 1, 14 and 21 days post-immunization. In contrast, Pereira [19] reported that the lymphocytes counts were not statistically different after oral vaccination in surubim hybrid (Pseudoplatystoma corruscans x P. reticulatum). However, the neutrophils and monocytes in the current study played a signi cant role in the early immune response followed by the lymphocytes.…”

“…To address the losses due to Streptococcosis and MAS, antibiotics are the only option for farmers and the use of antibiotics results in development of antibiotic resistance strains, bio-accumulation, changes the physio-chemical properties of water, imbalance of bacterial microbiota in sh body or in the habitat [17,18]. To solve these di culties, application of sh vaccines is an alternative to minimize the different infectious bacterial diseases [19].…”

Haemato-immunological responses and effectiveness of feed-based bivalent vaccine against Streptococcus iniae and Aeromonas hydrophila infections in hybrid red tilapia (Oreochromis spp.)

“…To address the losses due to Streptococcosis and MAS, antibiotics are the only option for farmers and the use of antibiotics results in development of antibiotic resistance strains, bio-accumulation, changes the physio-chemical properties of water, imbalance of bacterial microbiota in fish body or in the habitat [22][23][24]. To solve these difficulties, application of fish vaccines is an alternative to minimize the different infectious bacterial diseases [25,26].…”