Utilization of Water-Soluble Chitosan as Antiseptic Hand Sanitizer

Abstract

Shrimp wastes produced by manufacturers are mostly limited to be utilized by small industries. They only can turn the waste into chips and shrimp pastes even though it can be used as chitosan. The scarcity of shrimp waste utilized as chitosan is often constrained due to the lack of chitosan dissolve in water caused by the length of the molecular chain. In order to optimize the chitosan utilization, depolymerization is used; such as using H2O2. Chitosan depolymerization enables chitosan to be water soluble and has a high antibacterial ability, which can be applied as an antiseptic of hand sanitizer which has been derived from synthetic materials. The used method is an experimental method which includes the processing of making water-soluble chitosan with test parameters of deacetylation, solubility, moisture content, and yield. Furthermore, the manufacture of hand sanitizers is carried out in vitro and in vivo. The results showed that water-soluble chitosan can be made at 13% H2O2 concentration at 40°C with deacetylation degree 94.21%, 90% solubility, 10.60% moisture content and 3.5% yield. Moreover, after becoming a hand sanitizer, it was able to inhibit Escherichia coli and Staphylococcus epidermidis bacteria at concentrations of 120 mg/ml, respectively 19.53 mm and 21.12 mm, which were relatively strong. Resulted in MIC values 0.28% and 0.27%, and MBC values 1.12% and 1.08%, and also without causing irritation or edema on mouse skin.

Formulasi dan Pengujian Aktivitas Antibakteri Sediaan Gel Hand Sanitizer Berbahan Aktif Kitooligosakarida

“…Hand sanitizer formula 3 memiliki daya hambat yang paling optimal, hal ini menunjukkan bahwa sediaan hand sanitizer dengan konsentrasi kitooligosakarida yang semakin tinggi memiliki kemampuan penghambatan yang lebih baik terhadap pertumbuhan bakteri E. coli. Hal ini sesuai dengan penelitian Chamidah et al, (2019) yang menyatakan bahwa semakin tinggi konsentrasi kitooligosakarida dalam sediaan hand sanitizer, maka akan memicu kemampuan penghambatan bakteri yang semakin besar.…”

Depolimerisasi Kitosan dari Cangkang Udang dengan Enzim Papain dan Iradiasi Sinar Ultraviolet

“…Hasil rendemen oligokitosan sesuai dengan penelitian Jian dan Shen (2002) yang menyatakan bahwa kitosan dengan berat molekul yang rendah dapat menghasilkan rendemen sebesar 60-85%. Chamidah et al (2019) kali dari volume awalnya dapat menghasilkan rendemen yang optimal (Hidayah et al 2014).…”

Synthesis of Water Soluble Chitosan Inhibitor from Shrimp Shells and its Corrosion Rate on Mild Steel in Acidic Solution

“…The higher the concentration of H2O2 added, the higher the solubility level of WSCI will be. The high solubility is supported by the breaking of the glycosidic bonds in chitosan, so that the final molecular weight of the WSCI compound will be lighter (Chamidah et al, 2019). The event that occurs is that H2O2 will break down into radical hydroxyl groups, with the following mechanism:…”