Penelitian ini bertujuan untuk mendapatkan ekstrak kasar daun api-api putih yang memiliki aktivitas antioksidan terbaik, menentukan kandungan fitokimia dan efek Hepatoprotektif ekstrak kasar yang dipilih secara in vivo. Daun api-api putih diekstrak menggunakan tiga jenis pelarut, yakni metanol, etil asetat dan n-heksana. Dalam uji in vivo dilakukan evaluasi terhadap kadar malondialdehid (MDA), enzim aspartat transaminase (AST), enzim alanin transaminase (ALT), dan histopatologi. Hasil penelitian menunjukkan bahwa ekstraksi daun api-api putih dengan pelarut etil asetat menghasilkan aktivitas antioksidan terbaik dengan kandungan fitokimia terdiri dari flavonoid dan steroid/triterpenoid. Pemberian ekstrak etil asetat daun api-api putih pada tikus yang telah diinduksi CCl4 dapat menormalkan berbagai parameter biokimia stres oksidatif (kadar MDA, AST, dan ALT) dibandingkan dengan standar Silymarin. Ekstrak daun api-api putih berpotensi untuk melindungi hati tikus dari kerusakan oksidatif yang diinduksi CCl4. Efek Hepatoprotektif ekstrak daun api-api putih berkorelasi dengan aktivitas antioksidannya.<br />Kata kunci: antioksidan, Avicennia marina, ekstrak, hepatoprotektor

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Curcumin (Cur), a hydrophobic polyphenol compound, holds promising potential as an anticancer agent. However, the poor solubility in water and the low bioavailability of curcumin have limited its therapeutic applications. In this regard, we reported the formulation of curcumin using a solid-in-water (S/W) nanodispersion technique to enhance the water solubility and therapeutic activity of curcumin. This new aqueous formulation comprises simple preparation protocols: emulsi cation and freeze-drying for encapsulating hydrophobic biomolecules with a hydrophilic surfactant, followed by redispersion of the resultant solid complexes in an aqueous solution. Pluronics F68 and F127 were used here for the encapsulation of curcumin. Enhanced aqueous solubility of curcumin was achieved by encapsulating curcumin with a hydrophilic surfactant using the S/W nanodispersion technique. The resultant nanosized formulation had a narrow size distribution and high entrapment e ciency of curcumin. The highest loading capacity of curcumin in S/W nanodispersion was obtained with a weight ratio of curcumin to pluronic of 1 : 10 for both surfactants. The release pro le of the complexes was found to depend on the type of surfactant, suggesting that the selection of a proper surfactant is crucial for controlling curcumin delivery. The anticancer activity of the S/W formulation of curcumin was correlated with the drug release pro les and cellular uptake, which in turn was in uenced by the hydrophobicity and chemical structure of the surfactant.

Spirulina platensis is a microalga containing bioactive compound as antibacterial activity. The antibacterial compound from Spirulina can be used to treat bacterial infections on the skin such as acne. However, enhancement penetration of bioactive compounds through the skin was needed. The objective of this study was to determine bioactive compounds from Spirulina, synthesize Spirulina extract-water soluble chitosan nanoparticles using the ionic gelation method, and measure the antibacterial activity of Spirulina extract and its nanoparticles. For the synthesis of the nanoparticles, differences of Spirulina extract concentrations were used, including 3.25, 7.5, and 15 mg/mL. The yield of ethanolic Spirulina extract was 13.87±4.16% with the bioactive components of saponins, tannins, steroids, and phenols. The Spirulina extract-loaded chitosan nanoparticles were successfully fabricated with a nano-range size and narrow polydispersity. The amount of loading of Spirulina extract has affected the size and dispersion of nanoparticles. The antibacterial activity of nanoparticles showed higher than pure Spirulina extract only through Staphylococcus aureus and Pseudomonas aeruginosa, classified as moderate inhibition zones (5-10 mm). These suggested that nanoencapsulation could increase extract uptake on bacteria cells. The ethanolic Spirulina extract-chitosan nanoparticles have a potency for antibacterial agents on cosmeceutical products.