Uso del polimorfismo del gen de la β-caseína en términos de preservación del ganado lechero marrón

Ладыка, В. И.; Pavlenko, Y.; Sklyarenko, Y. I.

doi:10.21071/az.v70i269.5422

Cited by 8 publications

(3 citation statements)

References 18 publications

(28 reference statements)

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“…The local Lebedyn cattle (Ukraine) is characterized by a high frequency of the desired homozygous A2A2 genotype and A2 allele. This creates prerequisites for a high frequency of this genotype in the breed structure created on the basis of the Lebedyn breed by its holstinization (Ladyka, Pavlenko & Sklyarenko, 2021;Ladyka et al, 2019;Sklyarenko et al, 2018).…”

Section: Identification Of Polymorphism Of the Csn2 Gene Encoding Bet...mentioning

confidence: 99%

Identification of Polymorphism of the CSN2 Gene Encoding Beta-Casein in Ukrainian Black and White Breeds of Cattle

Skliarenko

2023

Acta fytotechn zootechn

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Licensed under a Creative Commons Attribution 4.0 International License Genotyping of 606 animals of the Ukrainian Black-and-White dairy breed was carried out in order to establish the features of genotype formation by beta-casein. It is established that the modern population of this breed has been formed using stud bulls of the Holstein breed, mainly of American and German breeding. It is found that the original breed has determined the features of polymorphism of the beta-casein gene. According to the research results, it is proved that the existing genetic structure of stud bulls of the Holstein and Ukrainian Black-and-White dairy breeds enables the formation of populations homozygous by these characteristics in subsequent generations. Among the breeding stock of the Ukrainian Black-and-White dairy breed, the frequency of the desired A2A2 genotype was 33-53%. It is expected to increase the frequency of occurrence of individuals with the A2A2 genotype by beta-casein in subsequent generations, especially if stud bulls homozygous by A2A2 beta-casein is used.

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Section: Identification Of Polymorphism Of the Csn2 Gene Encoding Bet...mentioning

confidence: 99%

Identification of Polymorphism of the CSN2 Gene Encoding Beta-Casein in Ukrainian Black and White Breeds of Cattle

Skliarenko

2023

Acta fytotechn zootechn

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“…The raw suitability of milk depends on the protein:fat ratio and the qualitative composition of casein. The β-casein composition of proteins is an important selection feature that affects the technical properties of milk [1,2].…”

Section: Introductionmentioning

confidence: 99%

Determining the influence of raw milk protein composition on the yield of cheese and its nutrient content

Ladyka,

Bolgova,

Synenko

et al. 2023

EEJET

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Cheese production is a complex process that is influenced by many factors: protein:fat ratio, acidity, and type of rennet. An option for improving the profitability of the cheese industry is the genetic selection of dairy cows to produce milk with good rennet protein coagulation. The object of the study is the technology of cheeses made from milk from cows with different β-casein genotypes (А1А1, А1А2, А2А2). The subject of the study is the physical-chemical parameters of milk from cows with different genotypes for β-casein; yield of cheese from this milk and its quality indicators. Samples of Gouda cheese were produced according to traditional technology. The research established that the quality indicators of milk samples are typical for fresh cow's milk. The content of fat, protein, and dry matter in the milk of cows with the β-casein genotype A2A2 were slightly higher compared to A1A1 and A1A2. The study of the quality indicators of the cheese samples showed that the type of β-casein did not affect the organoleptic properties of the cheese. However, according to the content of the main chemical components, cheeses made from A1A2 milk had a higher content of dry matter and protein (61,6 % and 19,2 % on average, respectively) and a lower fat content (37.2 %). The amino acid profile of cheese from milk of cows with β-casein A1A2 and A2A2 genotypes showed a higher total content of amino acids – 14.89 mg/g and 13.84 mg/g, respectively. Calculations of cheese yield showed that cheese yield from milk of cows with β-casein genotype A1A2 was higher (mean value 13.1 %) than with A1A1 and A2A2. The obtained results are of practical importance, as it is possible to take into account how changes in the β-casein genotype in milk can affect the yield of cheese, and therefore, the profitability of production

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“…Генотипування особин великої рогатої худоби за алелями А 1 та А 2 локусу бета-казеїну проводиться у різних регіонах світу, що вказує на дедалі зростаючий інтерес до цієї проблеми (Jiménez-Montenegro et al, 2022;Khan et al, 2023). В останні роки інтерес до генотипування особин ВРХ різних порід відмічений і в Україні, про що свідчить низка публікацій (Ladyka et al, 2021;Mokhnachova, 2021). При цьому, дослідження з оптимізації та підвищення ефективності методів типування особин ВРХ набуває все більшої актуальності на тлі загальної комерціалізації завдання в цілому.…”

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Genotyping of Cattle by Allelic Variants A1 and A2 of the Beta-Casein Gene: Employing Different Methodological Approaches (As-PCR and Acrs-Pcr)

Kulibaba,

Sakhatskyi,

Liashenko

2023

Anim. Breed. and Genet.

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This article addresses the comparative analysis of the efficiency of cattle genotyping based on allelic variants A1 and A2 of the beta-casein gene, employing different methodological approaches. The primary methods employed include AS-PCR (AS-PCR 244 bp and AS-PCR 854 bp) and ACRS-PCR (ACRS-PCR DdeI and ACRS-PCR TaqI). Bioinformatics and laboratory diagnostics methods were used for a comparative analysis of genotyping efficiency. The study results unveiled the advantages and disadvantages of each methodological approach employed, it identified the specificity and accuracy of flanking the experimental fragment of the bovine beta-casein gene and underscored the necessity to optimize typing algorithms based on prevailing conditions when utilizing model objects. Based on the results of the research, an effective general typing algorithm was developed using the AS-PCR and ACRS-PCR methods. The allele-specific PCR method is proposed as the primary approach for routine genotyping of cattle, with ACRS-PCR suggested as a tool to verify results in cases of ambiguous findings and for blind typing of samples, among other applications.

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Uso del polimorfismo del gen de la β-caseína en términos de preservación del ganado lechero marrón

Cited by 8 publications

References 18 publications

Identification of Polymorphism of the CSN2 Gene Encoding Beta-Casein in Ukrainian Black and White Breeds of Cattle

Identification of Polymorphism of the CSN2 Gene Encoding Beta-Casein in Ukrainian Black and White Breeds of Cattle

Determining the influence of raw milk protein composition on the yield of cheese and its nutrient content

Genotyping of Cattle by Allelic Variants A1 and A2 of the Beta-Casein Gene: Employing Different Methodological Approaches (As-PCR and Acrs-Pcr)

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