Genotyping of Cattle by Allelic Variants A1 and A2 of the Beta-Casein Gene: Employing Different Methodological Approaches (As-PCR and Acrs-Pcr)
R. O. Kulibaba,
M. I. Sakhatskyi,
Yu. V. Liashenko
Abstract:This article addresses the comparative analysis of the efficiency of cattle genotyping based on allelic variants A1 and A2 of the beta-casein gene, employing different methodological approaches. The primary methods employed include AS-PCR (AS-PCR 244 bp and AS-PCR 854 bp) and ACRS-PCR (ACRS-PCR DdeI and ACRS-PCR TaqI). Bioinformatics and laboratory diagnostics methods were used for a comparative analysis of genotyping efficiency. The study results unveiled the advantages and disadvantages of each methodologica… Show more
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