| As a result of molecular-genetic analysis of two cow populations -Ukrainian Brown Dairy breed (UBD) and Sumy interbreed type of Ukrainian Black-and-White breed (SITUBWD) in the technique of ISSR analysis with the use of the fragment of microsatellite loci with trinucleotide core motif (AGC) 6 C (S1) as an amplification primer, there were identified 23 DNA-patterns. It was determined that genetic locus with the identified size of 1350 bp was monomorphic in animals of SITUBWD occurring in all study samples, however, in UBD breed cows the amplicons of this size were not detected at all (p<0.001). The population-genetic analysis of the two studied animal groups (taking into account maternal origin) by molecular-genetic testing in the ISSR-PCR technique revealed essential statistically significant differences. The total average number of PCR products obtained from the cows of the newly developed Sumy interbreed type of Ukrainian Black-and-White breed was 15,050 being 12,450 in Ukrainian Brown Dairy breed (p <0,001). A rather high level of genetic similarity between the animals of Ukrainian Brown Dairy and Sumy interbreed type of Ukrainian Black-and-White Dairy (0,768) is quite possibly connected with the influence of the unique aboriginal Lebedynsky breed on which maternal base they were developed. citation | Ladyka V, Skliarenko Y, Pavlenko Y, Metlytska O, Ivankova I (2019). Molecular-genetic analysis of cows genetic structure and determination of genealogical relatedness level of bulls of modern dairy breeds. Adv. Anim. Vet. Sci. 7(5): 405-411. doI | http://dx.
Using real-time polymerase chain reaction methods, we have researched the CSN2 polymorphism (GenBank M55158; SNP rs43703011) gene in 4 breeds of cows in the North-East of Ukraine. The observed distributions of genotype frequencies А1/А1, А1/А2, А2/А2 and A1 or A2 alleles varies considerably in different cattle breeds: Lebedyn cattle - 14.6%, 31.7%, 53.7% (0.3 and 0.7); Ukrainian brown dairy breed - 6.8%, 43.2%, 50% (0.28 та 0.72); Simmental cattle - 7.3%, 51.2%, 41.5% (0.33 and 0.67); Ukrainian black speckled milky – 38.5%, 34.6%, 26.9% (0.56 та 0.44). The ratio of genotypes and alleles were found significant differences when comparing Lebedyn cattle and Simmental cattle (р=0.01), Lebedyn cattle and Ukrainian black speckled milky (р<0.0001), Lebedyn cattle compared to all other breeds (р=0.04), Ukrainian brown dairy breed and Ukrainian black speckled milky (р<0.0001), Ukrainian brown dairy breed compared to all other Ukrainian breeds (р=0.008); Simmental cattle and Ukrainian black speckled milky (р<0.0001), Simmental cattle (р=0.002) and Ukrainian black speckled milky (р<0.0001) to all other breeds. The obtained results indicate the prospects of breeding work to create herds with genotype A2/A2 animals - specifically with the Lebedyn cattle.
Licensed under a Creative Commons Attribution 4.0 International License Genotyping of 606 animals of the Ukrainian Black-and-White dairy breed was carried out in order to establish the features of genotype formation by beta-casein. It is established that the modern population of this breed has been formed using stud bulls of the Holstein breed, mainly of American and German breeding. It is found that the original breed has determined the features of polymorphism of the beta-casein gene. According to the research results, it is proved that the existing genetic structure of stud bulls of the Holstein and Ukrainian Black-and-White dairy breeds enables the formation of populations homozygous by these characteristics in subsequent generations. Among the breeding stock of the Ukrainian Black-and-White dairy breed, the frequency of the desired A2A2 genotype was 33-53%. It is expected to increase the frequency of occurrence of individuals with the A2A2 genotype by beta-casein in subsequent generations, especially if stud bulls homozygous by A2A2 beta-casein is used.
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