Use of MALDI-TOF mass spectrometry for rapid identification of group B Streptococcus on chromID Strepto B agar

Lu, Bo; Shi, Yanli; Zhang, Shuchen; Zhu, Fengcai; Li, Dong; Cui, Yan

doi:10.1016/j.ijid.2014.06.023

Cited by 19 publications

(17 citation statements)

References 15 publications

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“…Results of both molecular tests were compared with the reference standard of subculture onto solid media (ChromID StrepB agar; bioM erieux, Marcy l'Etoile, France) following overnight LIM broth enrichment. Culture plates were incubated for 18e24 h at 35 C. Species identification was confirmed by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (Bruker, Karlsruhe, Germany) [13]. In addition, the enriched LIM broth was also tested with the BD Max GBS (15 mL inoculated into the sample buffer tube).…”

Section: Methodsmentioning

confidence: 99%

Rapid detection of Group B streptococcus directly from vaginal–rectal specimens using liquid swabs and the BD Max GBS assay

Ellem

Kovacevic

Olma

et al. 2017

Clinical Microbiology and Infection

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The BD Max GBS assay is highly sensitive, requires minimal technical skill with <2 min required to set-up, and results are available in under 80 min (versus 24-48 h for culture). It is configured for 'on demand' testing and vaginal-rectal specimens can be rapidly screened for GBS without the need for enrichment. The results obtained in this study demonstrate that rapid GBS screening using the BD Max GBS assay at the time of delivery is a viable alternative to the current recommended screening at 35-37 weeks of gestation with pre-enrichment testing methods.

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Section: Methodsmentioning

confidence: 99%

Rapid detection of Group B streptococcus directly from vaginal–rectal specimens using liquid swabs and the BD Max GBS assay

Ellem

Kovacevic

Olma

et al. 2017

Clinical Microbiology and Infection

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“…After the enrichment culture, bacterial identification is performed, e.g., using the Christie–Atkins–Munch-Petersen test, serologic identification, growth on chromogenic agar, and nucleic acid amplification [ 4 ]. However, although a selective culture broth is used for the enrichment culture, S. agalactiae is poorly recovered along with overgrowth of Enterococcus faecalis in some cases [ 5 , 6 , 7 , 8 ]. This may lead to false-negative results in the subsequent identification tests [ 5 , 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, although a selective culture broth is used for the enrichment culture, S. agalactiae is poorly recovered along with overgrowth of Enterococcus faecalis in some cases [ 5 , 6 , 7 , 8 ]. This may lead to false-negative results in the subsequent identification tests [ 5 , 6 , 7 , 8 ]. To address this problem, selective antimicrobial agents to be included in the enrichment broth should be reevaluated.…”

Section: Introductionmentioning

confidence: 99%

Potential Application of Bacteriophages in Enrichment Culture for Improved Prenatal Streptococcus agalactiae Screening

Uchiyama

Matsui

Murakami

et al. 2018

Viruses

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Vertical transmission of Streptococcus agalactiae can cause neonatal infections. A culture test in the late stage of pregnancy is used to screen for the presence of maternal S. agalactiae for intrapartum antibiotic prophylaxis. For the test, a vaginal–rectal sample is recommended to be enriched, followed by bacterial identification. In some cases, Enterococcus faecalis overgrows in the enrichment culture. Consequently, the identification test yields false-negative results. Bacteriophages (phages) can be used as antimicrobial materials. Here, we explored the feasibility of using phages to minimize false-negative results in an experimental setting. Phage mixture was prepared using three phages that specifically infect E. faecalis: phiEF24C, phiEF17H, and phiM1EF22. The mixture inhibited the growth of 86.7% (26/30) of vaginal E. faecalis strains. The simple coculture of E. faecalis and S. agalactiae was used as an experimental enrichment model. Phage mixture treatment led to suppression of E. faecalis growth and facilitation of S. agalactiae growth. In addition, testing several sets of S. agalactiae and E. faecalis strains, the treatment with phage mixture in the enrichment improved S. agalactiae detection on chromogenic agar. Our results suggest that the phage mixture can be usefully employed in the S. agalactiae culture test to increase test accuracy.

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“…Using the selected primer set, the species-specific cAMP gene of S. agalactiae was amplified and detected without magnification in 40 minutes. While culture detection requires 36 -72 hours (6,15) and antigen detection or PCR-based assay need over two hours (7,(16)(17)(18). Secondly, LAMP method has high sensitivity for detection of S. agalactiae.…”

Section: Discussionmentioning

confidence: 99%

Rapid Detection of Streptococcus agalactiae Infection Using a Loop-Mediated Isothermal Amplification Method

Yang

Cao

Wang

et al. 2016

Jundishapur J Microbiol

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Background: Streptococcus agalactiae colonization in pregnant women leads to prenatal and neonatal infections worldwide; thus, early detection is very crucial. Objectives: Development of a rapid detection method for S. agalactiae. Methods: Genomic DNA of cultured S. agalactiae was prepared and loop-mediated isothermal amplification (LAMP) primers were designed based on the cAMP gene in bacteria. The optimum primer set was selected based on the reaction speed and specificity. The reaction result was monitored visually. The sensitivity and specificity of the LAMP method were evaluated and compared with polymerase chain reaction (PCR) method. Results: Using the optimum primer set the reaction can be completed in 40 minutes at 63°C in a water bath. The LAMP assay was 10 -100 times more sensitive than PCR, with a detection limit of 10 CFU/mL of S. agalactiae. Forty vaginal swab were examined by LAMP, and the specificity was 100%. Conclusions: Thus, for S. agalactiae detection, the LAMP method is a valuable diagnostic tool, with easy, rapid, visual, accurate, and sensitive advantages.

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Use of MALDI-TOF mass spectrometry for rapid identification of group B Streptococcus on chromID Strepto B agar

Abstract: This study demonstrated that STRB combined with MALDI-TOF MS is a fast, sensitive, and accurate method for the identification of GBS-colonized pregnant women.

Cited by 19 publications

References 15 publications

Rapid detection of Group B streptococcus directly from vaginal–rectal specimens using liquid swabs and the BD Max GBS assay

Rapid detection of Group B streptococcus directly from vaginal–rectal specimens using liquid swabs and the BD Max GBS assay

Potential Application of Bacteriophages in Enrichment Culture for Improved Prenatal Streptococcus agalactiae Screening

Rapid Detection of Streptococcus agalactiae Infection Using a Loop-Mediated Isothermal Amplification Method

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