pJIE143 (34 kb), from an Escherichia coli ST131 isolate, carries bla CTX-M-15 but could not be typed using the standard PCR-based replicon-typing primer set. Complete sequencing revealed a backbone with similarity to IncX plasmids, including a pir-like gene encoding a -like replication protein and iterons related to those of other IncX plasmids. The 2.971-kb ISEcp1-bla CTX-M-15 -orf477⌬ transposition unit often found within Tn2 is inserted just beyond the end of pir, flanked by 5-bp direct repeats.
The number and diversity of genes potentially complicate genetic approaches to the rapid detection of transmissible extended-spectrum -lactamase genes. We developed a robust multiplexed real-time PCR assay based on targets identified in a prior survey and used this to detect relevant genes in 617 consecutive clinical isolates of extended-spectrum -lactamase (ESBL)-producing Enterobacteriaceae.
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