Rapid detection of Group B streptococcus directly from vaginal–rectal specimens using liquid swabs and the BD Max GBS assay

Ellem, Justin A.; Kovacevic, Daniel; Olma, Tom; Chen, Sharon C‐A

doi:10.1016/j.cmi.2017.04.022

Cited by 14 publications

(10 citation statements)

References 27 publications

(17 reference statements)

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“…Recently, Ellem et al . also demonstrated that the direct BD Max GBS assay offered viable alternative to the current CDC recommended screening culture‐based method, but they detected one more GBS isolates by enrichment culture than direct BD Max assay unlike that found in the present study …”

Section: Discussioncontrasting

confidence: 80%

Detection and genomic analysis of genital group B streptococcus in pregnant Korean women

Lee

Kim

Park

et al. 2018

J of Obstet and Gynaecol

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Section: Discussioncontrasting

confidence: 80%

Detection and genomic analysis of genital group B streptococcus in pregnant Korean women

Lee

Kim

Park

et al. 2018

J of Obstet and Gynaecol

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“…It was found that the rapid GBS screening using the BD Max GBS assay, even at the time of delivery, is a viable alternative to the current recommended screening procedures at 35e37 weeks of gestation with pre-enrichment testing methods. Moreover, Ellem et al claim that the vaginalerectal specimen can be rapidly screened for GBS presence, directly after collection, without the need for its enrichment, reaching the sensitivity of 98.4%, compared to 100% sensitivity when it followed the broth enrichment culture [6].…”

Section: Discussionmentioning

confidence: 99%

Application of the appropriate molecular biology-based method significantly increases the sensitivity of group B streptococcus detection results

Bogiel

Depka

Zalas‐Więcek

et al. 2021

Journal of Hospital Infection

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Background: Streptococcus agalactiae (group B streptococcus: GBS) is a leading cause of early-and late-onset diseases in neonates. Reliable results of GBS carriage investigation among pregnant women may decrease the incidence of neonatal infection and mortality. Aim: To compare the results of conventional culture investigation with those of the US Food and Drug Administration-approved nucleic acid amplification test (BD Max GBS (Becton Dickinson)), and to establish our own protocols of standard polymerase chain reaction (PCR). Methods: A total of 250 vaginalerectal swabs from three different hospitals in Bydgoszcz, Poland, were used to evaluate GBS carriage. Standard laboratory technique (overnight culture in broth enrichment media) results were compared with those of BD Max GBS assay (Becton Dickinson) and two standard PCR protocols, established to detect the cfb and 16S rRNA S. agalactiae genes, from the overnight cultures of the samples in the liquid enrichment media. Findings: The overall GBS carriage was estimated as 16.4e23.2%, depending on the applied detection method. The highest percentage of positive results, from each laboratory was obtained with the application of BD Max GBS assay. The differences in the number of positive results obtained with this particular method were statistically significant. Overall, 27 discrepancies were noted for the results obtained with the application of the methods compared. Conclusions: The methods applied for GBS detection differ in sensitivity. A culture technique, though very specific, appears to be less sensitive at detecting S. agalactiae compared with the commercially available BD Max GBS assay or in-house PCR protocols established for this purpose.

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“…Notably, the discrepancy analysis seems to indicate that both PCR assays were affected by freeze-thawing because some of the BD MAX samples that were initially positive, changed status to negative despite a high number of GBS-colony-forming units in the samples, based on the semi-quantitative culture results. It is also relevant to note that, in a recent clinical study using fresh vaginal-rectal swab specimens, the BD MAX GBS PCR assay detected 61 specimens with and 62 without enrichment culture, respectively, among 62 culture-positive specimens[13].…”

Section: Discussionmentioning

confidence: 99%

“…The difference in run time between the BD MAX and GenomEra PCR assays is only one hour (120 minutes vs. 60 minutes, respectively). Notably, the BD GeneOhm StrepB assay is a rapid and sensitive PCR assay (~ 1 hour) [13]. However, for optimal performance, the BD system still requires specially trained laboratory staff.…”

Section: Discussionmentioning

confidence: 99%

Comparison of BD MAX GBS and GenomEra GBS assays for rapid intrapartum PCR detection of vaginal carriage of group B streptococci

2019

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Objective To compare the diagnostic performance of BD MAX and GenomEra PCR assays for a rapid PCR detection of vaginal carriage of group B streptococci at delivery. Methods This is a retrospective laboratory analysis of vaginal swab samples taken intrapartum from a randomly selected cohort of pregnant women giving birth at a single childbirth and maternity unit. Results Ninety-one culture-positive and 279 culture-negative vaginal samples were included from a cohort of 902 women. One-hundred-and-two specimens were found positive with the BD MAX and 84 with the GenomEra PCR assay. No statistically significant difference was observed compared to culture, sensitivity of BD MAX 84.6% (77/91) [95%CI 75.5–91.3] and of GenomEra 71.4% (65/91) [95%CI 61.0–80.4]. When compared to a combined reference standard, no statistically significant differences were seen between culture, BD MAX and GenomEra PCR assays. The sensitivities were 82.7% (91/110) [95%CI 74.3–89.3], 87.3% (96/110) [95%CI 79.6–92.9], and 79.1% (87/110) [95%CI 70.3–86.3], respectively. Conclusion Both PCR assays performed comparably to culture of the intrapartum vaginal samples. In particular, the GenomEra assay is potentially an easy and rapid on-site PCR test for intrapartum detection of vaginal carriage of group B streptococci at a maternity ward to identify women who should receive intrapartum antibiotic prophylaxis.

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Rapid detection of Group B streptococcus directly from vaginal–rectal specimens using liquid swabs and the BD Max GBS assay

Cited by 14 publications

References 27 publications

Detection and genomic analysis of genital group B streptococcus in pregnant Korean women

Detection and genomic analysis of genital group B streptococcus in pregnant Korean women

Application of the appropriate molecular biology-based method significantly increases the sensitivity of group B streptococcus detection results

Comparison of BD MAX GBS and GenomEra GBS assays for rapid intrapartum PCR detection of vaginal carriage of group B streptococci

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