Use of AFLP molecular markers for estimating genetic similarity of alfalfa (Medicago sativa L. Sl.)

Abstract

Use of AFLP molecular markers for estimating genetic similarity of alfalfa (Medicago sativaL. Sl.)The aim of study was to determine genetic similarity among sixteen alfalfa populations using the AFLP technique. Plant material was selected considering the high genotypic variability. It included populations of different origin: native forms of alfalfa (secondary ecotypes), inbred lines, single hybrids, synthetic populations, varieties and mutants. Analysis was carried out using 10 combinations of primers. It was noticed, that in case of the studied alfalfa populations, the highest polymorphism was obtained using primers with three selective nucleotides. The genetic similarity coefficients were calculated by the method of Nei and Li. Those coefficients were used for the hierarchical grouping of objects by the unweighted pair group method of arithmetic means. Results of the grouping were presented in the form of dendrogram. Values of coefficients ranged from 0.3311 (between the inbred F line and the Ulstar variety) to 0.8076 (between ecotype 27 and the synthetic population Syn 7-3). Dendrogram of genetic similarity obtained in this way did not fully correspond with the known data about the studied populations' origin. Considering the genetic similarity, inbreed line F was the most outlying from the studied populations of alfalfa.

Pheno-morphological variation, genetic diversity and population structure of Tunisian Echinus Medic (Medicago ciliaris L.)

“…The latter primer was previously used in studies assessing genetic diversity of alfalfa populations and clover species. For alfalfa populations, this primer combination produced the highest mean number of polymorphic fragments (29.9) per population, which was considered relatively low compared to the results of other studies in the same or related species according to Dobrzycka et al (2009). In addition, for clover species, this primer combination amplified 52 bands across four studied populations, which was the lowest number among the four primers used (Bennett and Mathews, 2003).…”

Assessment of Genetic Relationships in Breeding Lines and Cultivars of Brassica napus and Their Implications for Breeding Winter Oilseed Rape

“…This technique is a highly reproducible method with low error rates ( Jones et al 1997;Powell et al 1996). Amplified fragment length polymorphism markers have been widely used in many plant species to construct genetic maps, to study genetic diversity phylogenic relations and crop evolution, to evaluate germplasm, and to reveal differences between varieties and genotypes (Lübberstedt et al, 2000;Lombard and Delourme, 2001;Hartings et al, 2008;Pawłowicz et al, 2008;Dobrzycka et al, 2009;Bocianowski et al, 2011;Kozak et al, 2011;Liersch et al, 2013;Würschum et al, 2013). Lombard et al (2000), Negi et al (2004), and Faltusová et al (2011) reported that AFLP markers demonstrated the high efficiency of the AFLP technique for genome analysis in Brassica.…”