Unique Actinomycetes from Marine Caves and Coral Reef Sediments Provide Novel PKS and NRPS Biosynthetic Gene Clusters

Hodges, Tyler W.; Slattery, Marc; Olson, Julie B.

doi:10.1007/s10126-011-9410-7

Cited by 41 publications

(35 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…NRPS biosynthetic genes were identified in 19 isolates (76%), while PKS-I genes were detected in 12 strains (48%), and PKS-II genes in eight strains (32%). NRPS and PKS sequence diversity have been reported in marine actinomycetes isolated from different marine environments including marine caves, marine sediments, and marine sponges, where these sequences were detected in up to 90% of the tested strains [46,47]. …”

Section: Resultsmentioning

confidence: 99%

Actinomycetes from Red Sea Sponges: Sources for Chemical and Phylogenetic Diversity

et al. 2014

View full text Add to dashboard Cite

The diversity of actinomycetes associated with marine sponges collected off Fsar Reef (Saudi Arabia) was investigated in the present study. Forty-seven actinomycetes were cultivated and phylogenetically identified based on 16S rRNA gene sequencing and were assigned to 10 different actinomycete genera. Eight putatively novel species belonging to genera Kocuria, Mycobacterium, Nocardia, and Rhodococcus were identified based on sequence similarity values below 98.2% to other 16S rRNA gene sequences available in the NCBI database. PCR-based screening for biosynthetic genes including type I and type II polyketide synthases (PKS-I, PKS-II) as well as nonribosomal peptide synthetases (NRPS) showed that 20 actinomycete isolates encoded each at least one type of biosynthetic gene. The organic extracts of nine isolates displayed bioactivity against at least one of the test pathogens, which were Gram-positive and Gram-negative bacteria, fungi, human parasites, as well as in a West Nile Virus protease enzymatic assay. These results emphasize that marine sponges are a prolific resource for novel bioactive actinomycetes with potential for drug discovery.

show abstract

Section: Resultsmentioning

confidence: 99%

Actinomycetes from Red Sea Sponges: Sources for Chemical and Phylogenetic Diversity

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The antimicrobial potential of cave actinobacteria, and especially Streptomyces, has received the attention of many scientists and it is a topic of great interest (Herold et al, 2005;Hodges et al, 2012;Cheeptham, 2013;Nimaichand et al, 2015).…”

Section: Antimicrobial Activity Of Actinomycetesmentioning

confidence: 99%

“…From that time on, a number of papers reported the isolation of cave actinobacteria with production of bioactive compounds (Nakaew et al, 2009;Hodges et al, 2012;Cheeptham et al, 2013;Ghosh et al, 2016;Maciejewska et al, 2016). The most promising was the discovery of cervimycin A-D, a polyketide complex produced by a strain of Streptomyces tendae, isolated from Grotta dei Cervi, in Italy.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activities of culturable microorganisms (actinomycetes and fungi) isolated from Chaabe Cave, Algeria

Belyagoubi¹,

Belyagoubi-Benhammou²,

Jurado³

et al. 2018

IJS

View full text Add to dashboard Cite

Abstract:The discovery of new antibiotics and other bioactive microbial metabolites is a priority given the frequency of the emerging multi-drug resistant pathogenic microorganisms. Thus, scientists are searching for new antibiotics in microorganisms isolated from extreme habitats such as caves. In the present study, we aimed to perform the first analysis on culturable microorganisms in the Chaabe Cave (Algeria), and to test the antimicrobial activities of the isolates (Streptomyces spp. and Penicillium spp.). The potential for antimicrobial activity of 47 strains of actinomycetes and 23 strains of fungi were tested on Candida albicans, Staphylococcus aureus, Micrococcus luteus, Listeria monocytogenes, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa. The identification of the most active actinomycetes strains by 16S rRNA gene sequence analysis revealed that these isolates were exclusively affiliated to the genus Streptomyces. On the other hand, the fungi were determined by phylogenetic analysis based on the ITS region or on the β tubulin gene and were affiliated to the genera Readeriella, Cladosporium, Aspergillus, Penicillium, Beauveria, Alternaria, Rhizopus, and Rhizomucor. The strains showed strong inhibitory activity against pathogenic microorganisms and the diameters of the inhibition zones vary between 7.5 and 34 mm for Streptomyces strains, and between 6.5 and 19.50 mm for Penicillium strains. The data indicated that the majority (72.86%) of the 70 isolates were active against at least one of the tested microorganisms. The production of nonpolyenic antifungal substances by active Streptomyces isolates was investigated using several criteria (antibacterial activity, ergosterol inhibition, and UV-visible spectra) of active extracts. The results were promising and showed that the metabolites produced by the actinomycete strains do not have a UVvisible spectrum characteristic of a polyenic structure. The Chaabe Cave possesses a diversity of microorganisms that could lead to new antibiotics necessary in the fight against drug-resistant pathogens and warrant further study.

show abstract

“…Freshwater ecosystems have been reported as promising source of bioactive actinomycetes (Hodges et al, 2012).…”

Section: Actinomycetes In Aquatic Environmentsmentioning

confidence: 99%

Reappraisal of actinomycetes for novel bioactive metabolites

Shah¹,

Rasool²,

Majeed³

et al. 2017

View full text Add to dashboard Cite

The appearance of new deadly diseases like cancer and the burgeoning problem of drug resistance among common bacteria l pathogens are a serious threat to a vailable trea tments. Since the channels of compounds under development are limited, this necessitates the discovery of new drugs. It is where actinomycetes can complement in the accomplishment of development of thera peu tically new bioactive compounds, predomina ntly u sed in antibiotic production. Actinomycetes are diverse in their location and have proven ability to produce new bioactive compounds. By employing modern microbiological and molecular technologies, the target-directed search for detection and isolation of bioactive actinomycetes is gaining more strength. Therefore, the innova tive isolation of actinomycetes from extreme ecosystems, their identification and cultivation using novel techniques are imperative to pursue for drug discovery.

show abstract

Unique Actinomycetes from Marine Caves and Coral Reef Sediments Provide Novel PKS and NRPS Biosynthetic Gene Clusters

Cited by 41 publications

References 57 publications

Actinomycetes from Red Sea Sponges: Sources for Chemical and Phylogenetic Diversity

Actinomycetes from Red Sea Sponges: Sources for Chemical and Phylogenetic Diversity

Antimicrobial activities of culturable microorganisms (actinomycetes and fungi) isolated from Chaabe Cave, Algeria

Reappraisal of actinomycetes for novel bioactive metabolites

Contact Info

Product

Resources

About