Reappraisal of actinomycetes for novel bioactive metabolites

Shah, Aabid Manzoor; Rasool, Shahid; Majeed, Aasif; Mushtaq, Saleem; Khan, M. H.; Hussain, Aashiq; Shah, Aabid Manzoor; Hassan, Qazi Parvaiz

doi:10.21276/ap.2017.6.1.3

Cited by 3 publications

(2 citation statements)

References 51 publications

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“…The infection by PVY results in a wide range of symptoms, such as vein necrosis and systematic mottles, with necrotic ringspots reducing crop yield and quality substantially (Chen et al, 2017). Upon infection, plants activate their defense mechanisms by producing various resistant substances, including phytoalexin chitinase, peroxidase, and plant antitoxin to enhance their tolerance (Shah et al, 2017). Furthermore, the presence of PVY in a host organism leads to the observation of numerous morphological, physiological, and histological alterations (Hinrichs-Berger et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Rhizosphere microbial community enrichment processes in healthy and diseased plants: implications of soil properties on biomarkers

Deng,

Kong,

Zhang

et al. 2024

Front. Microbiol.

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Plant health states may influence the distribution of rhizosphere microorganisms, which regulate plant growth and development. In this study, the response of rhizosphere bacteria and fungi of healthy and diseased plants compared to bulk microbes was analyzed using high-throughput sequencing. Plant adaptation strategies of plants under potato virus Y (PVY) infection have been studied from a microbial perspective. The diversity and community structure of bacteria and fungi varied between bulk and rhizosphere soils, but not between healthy and diseased rhizosphere soils. A LEfSe analysis revealed the significant differences between different treatments on bacterial and fungal community compositions and identified Roseiflexaceae, Sphingomonas, and Sphingobium as the bacterial biomarkers of bulk (BCK), healthy rhizosphere (BHS), and diseased rhizosphere (BIS) soils, respectively; Rhodotorula and Ascomycota_unidentified_1_1 were identified as the fungal biomarkers of bulk (FCK) and healthy rhizosphere (FHS) soils. Bacterial networks were found to be more complex and compact than fungal networks and revealed the roles of biomarkers as network keystone taxa. PVY infection further increased the connectedness among microbial taxa to improve rhizosphere microbial community stability and resistance to environmental stress. Additionally, water content (WC) played an apparent influence on bacterial community structure and diversity, and pH showed significant effects on fungal community diversity. WC and pH greatly affected the biomarkers of bacterial rhizosphere communities, whereas the biomarkers of bulk bacterial communities were significantly affected by soil nutrients, especially for Sphingobium. Overall, the rhizosphere microbial community enrichment processes were different between healthy and diseased plants by changing the community compositions and identifying different biomarkers. These findings provide insight into the assemblage of rhizosphere microbial communities and soil physicochemical properties, which contributes to a deeper understanding of the establishment of an artificial core root microbiota to facilitate plant growth and bolstering resistance mechanisms. This knowledge contributes to a deeper understanding of the establishment of an artificial core root microbiota, thereby facilitating plant growth and bolstering resistance mechanisms.

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Section: Introductionmentioning

confidence: 99%

Rhizosphere microbial community enrichment processes in healthy and diseased plants: implications of soil properties on biomarkers

Deng,

Kong,

Zhang

et al. 2024

Front. Microbiol.

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“…Therapies of fungal infections have been restricted by the limited numbers, low safety profiles and emergence of resistance to currently used antifungal agents [ 15 ]. No doubt we are facing a serious public health problem, which highlights the crucial need for the discovery of new specific antifungal agents, preferably from natural resources, particularly those that have not been exploited yet [ 16 ], for instance, soil microbial communities, which have been reported to produce biologically active natural byproducts [ 17 , 18 , 19 , 20 ]. This study is concerned with Lysinibacillus sp., a Gram-positive, rod-shaped bacterium from the family Bacillaceae [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Lysinibacillus Isolate MK212927: A Natural Producer of Allylamine Antifungal ‘Terbinafine’

et al. 2021

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Resistance to antifungal agents represents a major clinical challenge, leading to high morbidity and mortality rates, especially in immunocompromised patients. In this study, we screened soil bacterial isolates for the capability of producing metabolites with antifungal activities via the cross-streak and agar cup-plate methods. One isolate, coded S6, showed observable antifungal activity against Candida (C.) albicans ATCC 10231 and Aspergillus (A.) niger clinical isolate. This strain was identified using a combined approach of phenotypic and molecular techniques as Lysinibacillus sp. MK212927. The purified metabolite displayed fungicidal activity, reserved its activity in a relatively wide range of temperatures (up to 60 °C) and pH values (6–7.8) and was stable in the presence of various enzymes and detergents. As compared to fluconazole, miconazole and Lamisil, the minimum inhibitory concentration of the metabolite that showed 90% inhibition of the growth (MIC90) was equivalent to that of Lamisil, half of miconazole and one fourth of fluconazole. Using different spectroscopic techniques such as FTIR, UV spectroscopy, 1D NMR and 2D NMR techniques, the purified metabolite was identified as terbinafine, an allylamine antifungal agent. It is deemed necessary to note that this is the first report of terbinafine production by Lysinibacillus sp. MK212927, a fast-growing microbial source, with relatively high yield and that is subject to potential optimization for industrial production capabilities.

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Antitumor Microbial Products by Actinomycetes Isolated from Different Environments

Guimarães

Gomes

Fernandes

et al. 2020

Microorganisms for Sustainability

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Reappraisal of actinomycetes for novel bioactive metabolites

Cited by 3 publications

References 51 publications

Rhizosphere microbial community enrichment processes in healthy and diseased plants: implications of soil properties on biomarkers

Rhizosphere microbial community enrichment processes in healthy and diseased plants: implications of soil properties on biomarkers

Lysinibacillus Isolate MK212927: A Natural Producer of Allylamine Antifungal ‘Terbinafine’

Antitumor Microbial Products by Actinomycetes Isolated from Different Environments

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