Ultra‐thin, high strength, antibiotic‐eluting sutures for prevention of ophthalmic infection

Parikh, Kalpesh; Omiadze, Revaz; Josyula, Aditya; Shi, Richard; Anders, Nicole M.; He, Ping; Yazdi, Youseph; McDonnell, Peter J.; Ensign, Laura M.

doi:10.1002/btm2.10204

Cited by 21 publications

(10 citation statements)

References 66 publications

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“…Prevention and treatment of ocular infection in rats was assessed using a previously described model with minor adjustments. 56 S. aureus bacteria were propagated on nutrient agar plates followed by incubation at 37°C for 16 h. The bacterial colonies were collected and suspended in sterile PBS to obtain bacterial count of 1 × 10 8 CFU/ml. Rats received general anesthesia prior to applying a drop of 0.5% proparacaine hydrochloride ophthalmic solution to the cornea.…”

Section: Methodsmentioning

confidence: 99%

An ion‐paired moxifloxacin nanosuspension eye drop provides improved prevention and treatment of ocular infection

Josyula

Omiadze

Parikh

et al. 2021

Bioengineering & Transla Med

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There are numerous barriers to achieving effective intraocular drug administration, including the mucus layer protecting the ocular surface. For this reason, antibiotic eye drops must be used multiple times per day to prevent and treat ocular infections. Frequent eye drop use is inconvenient for patients, and lack of adherence to prescribed dosing regimens limits treatment efficacy and contributes to antibiotic resistance.Here, we describe an ion-pairing approach used to create an insoluble moxifloxacinpamoate (MOX-PAM) complex for formulation into mucus-penetrating nanosuspension eye drops (MOX-PAM NS). The MOX-PAM NS provided a significant increase in ocular drug absorption, as measured by the area under the curve in cornea tissue and aqueous humor, compared to Vigamox in healthy rats. Prophylactic and treatment efficacy were evaluated in a rat model of ocular Staphylococcus aureus infection. A single drop of MOX-PAM NS was more effective than Vigamox, and completely prevented infection. Once a day dosing with MOX-PAM NS was similar, if not more effective, than three times a day dosing with Vigamox for treating Aditya Josyula and Revaz Omiadze contributed equally to this study.

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Section: Methodsmentioning

confidence: 99%

An ion‐paired moxifloxacin nanosuspension eye drop provides improved prevention and treatment of ocular infection

Josyula

Omiadze

Parikh

et al. 2021

Bioengineering & Transla Med

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“…In a 2020 study, a drug-eluting suture was developed for ocular delivery of levofloxacin which indicated a 30-days drug release and efficacy against bacterial keratitis in rat eyes. 51 Mirzaeei et al designed nanofibrous matrices for ocular delivery of ofloxacin indicated the sustained release of drug through 96 h and improved pharmacokinetics compared to the drug solution. 52 An enhanced in vitro antibacterial activity was reported by levofloxacin ocular nanospheres in another study.…”

Section: In Vitro Release and Kineticsmentioning

confidence: 99%

Development of Chitosan–Tripolyphosphate Nanoparticles as Glycopeptide Antibiotic Reservoirs and Ex vivo Evaluation for Their Potential to Enhance the Corneal Permeation in Ocular Drug Delivery

Safari¹,

Mirzaeei²,

Mohammadi³

2021

Pharm Sci

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Purpose: The present investigation aimed to prepare Vancomycin-loaded nanoparticles (VAN-NPs) using chitosan (CS) and tripolyphosphate (TPP) besides exploring the effects of changing CS/TPP ratio on the physicochemical properties, corneal permeation, and ocular delivery of the prepared NPs. Methods: Different pre-formulations were prepared using the modified ionic gelation process, then were characterized in terms of size distribution. Optimized formulations were furtherly evaluated by some characteristic tools such as Fourier-transform infrared (FTIR) spectroscopy and thermogravimetric analysis (TGA). The in vitro antimicrobial efficacy and drug release amounts along with the Ex-vivo corneal permeation of NPs through the sheep cornea were investigated. Quantification was performed using High-Performance Liquid Chromatography. Results: Spherical and uniformly distributed NPs were developed with a mean particle size varied between 215–290 nm. FTIR spectroscopy confirmed that the CS/TPP cross-linking has taken place without affecting the pharmacologically active moiety of the drug. The obtained zeta potential values were in the range of +34 to +37 mV, which could ensure the stability of formulations. TGA analysis indicated enhanced thermal stability for the encapsulated drug compared to the plain drug. Formulations indicated suitable antimicrobial efficacy while releasing more than 90% of the drug during 24 h. NPs offered a 10-fold enhancement in corneal permeation compared to the drug solution. Conclusions: Although further in vivo evaluation is still required to completely confirm the efficacy of the formulations, the enhanced release and corneal permeation of the drug suggest that the prepared NPs are suitable for ocular delivery of VAN.

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“…The growing demand for versatile, biocompatible synthetic polymers in biomedical engineering and medical devices has galvanized research beyond the biocompatibility and tissue‐regenerative ability of FDA‐approved synthetic polymers such as polycaprolactone (PCL), poly(methyl methacrylate) (PMMA), poly(lactic‐co‐glycolic acid) (PLGA), and polyurethanes (PU) and toward the design of more tailorable polymeric materials with enhanced biological and mechanical properties 1–5 . PU‐based polymers have recently attracted substantial interest for use in various tissue‐regenerative processes, including muscle, cartilage, blood vessel and bone regeneration, because of their tunable properties, such as biodegradability, elasticity and resistance to flex fatigue 6–10 .…”

Section: Introductionmentioning

confidence: 99%

Hyperelastic, shape‐memorable, and ultra‐cell‐adhesive degradable polycaprolactone‐polyurethane copolymer for tissue regeneration

Hong

Yoon

Cha

et al. 2022

Bioengineering & Transla Med

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Novel polycaprolactone-based polyurethane (PCL-PU) copolymers with hyperelasticity, shape-memory, and ultra-cell-adhesion properties are reported as clinically applicable tissue-regenerative biomaterials. New isosorbide derivatives (propoxylated or ethoxylated ones) were developed to improve mechanical properties by enhanced reactivity in copolymer synthesis compared to the original isosorbide. Optimized PCL-PU with propoxylated isosorbide exhibited notable mechanical performance (50 MPa tensile strength and 1150% elongation with hyperelasticity under cyclic load). The shape-memory effect was also revealed in different forms (film, thread, and 3D scaffold) with 40%-80% recovery in tension or compression mode after plastic deformation. The ultra-cell-adhesive property was proven in various cell types which were reasoned to involve the heat shock protein-mediated integrin (α5 and αV) activation, as analyzed by RNA sequencing and inhibition tests. After the tissue regenerative potential (muscle and bone) was confirmed by the myogenic and osteogenic Suk-Min Hong, Ji-Young Yoon, and Jae-Ryung Cha contributed equally to this work.

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Ultra‐thin, high strength, antibiotic‐eluting sutures for prevention of ophthalmic infection

Abstract: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process which may lead to differences between this version and the Version of Record. Please cite this article as

Cited by 21 publications

References 66 publications

An ion‐paired moxifloxacin nanosuspension eye drop provides improved prevention and treatment of ocular infection

An ion‐paired moxifloxacin nanosuspension eye drop provides improved prevention and treatment of ocular infection

Development of Chitosan–Tripolyphosphate Nanoparticles as Glycopeptide Antibiotic Reservoirs and Ex vivo Evaluation for Their Potential to Enhance the Corneal Permeation in Ocular Drug Delivery

Hyperelastic, shape‐memorable, and ultra‐cell‐adhesive degradable polycaprolactone‐polyurethane copolymer for tissue regeneration

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