Ultra-performance liquid chromatography–tandem mass spectrometric method for the determination of Artemisinin in rat serum and its application in pharmacokinetics

A rapid and simple method based on ultra-performance liquid chromatography on a hydrophilic interaction chromatography column with tandem mass-selective detection (UPLC-MS/MS) to determine meldonium in human plasma was developed. The calibration curve acquired in the range of 10-6000 ng/mL had quadratic form. Method validation proved the conformity of its properties (selectivity, matrix effect, lower limit of quantification, accuracy, precision and recovery) with the established requirements. The stability tests necessary for bioanalytical studies were performed. For the first time, the method was successfully applied to the bioequivalence studies of generic and brand name oral drugs of meldonium in capsules. Based on data from 24 volunteers, it was determined that the mean pharmacokinetic curves of the drugs are characterized by a double peak profile.

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“…The advantages of UPLC‐MS/MS for pharmacokinetic studies as compared with traditional HPLC are described in Li et al . ().…”

Section: Introductionmentioning

confidence: 99%

UPLC‐MS/MS method for bioequivalence study of oral drugs of meldonium

Pidpruzhnykov

Sabko

Iurchenko

et al. 2011

Biomedical Chromatography

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“…Ultra-performance LC system has been employed in pharmacokinetic research [22,23], metabonomics study [24,25] and analysis of multiple compounds in herbal prescription [26]. By using 1.8 m rapid resolution high throughput (RRHT) columns on common liquid chromatography system, it is possible to achieve rapid analysis in an economic manner.…”

mentioning

confidence: 99%

Simultaneous determination of 6-gingerol, 8-gingerol, 10-gingerol and 6-shogaol in rat plasma by liquid chromatography–mass spectrometry: Application to pharmacokinetics

Wang

Wen

et al. 2009

Journal of Chromatography B

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“…An improvement in chromatographic performance has been achieved by the introduction of ultra-performance liquid chromatography (UPLC). Thus, UPLC takes full advantage of chromatographic principles to run separations using columns packed with smaller particles (1.7 m), with superior resolution and a shorter analysis time [21]. A 50 mm column subjected to an isocratic flow rate of 0.3 mL/min for 3 min was used for the chromatographic separation.…”

Section: Spiked Concentration (Ng/ml)mentioning

confidence: 99%

“…This might suggest that ARB-89 quickly leaves the central compartment and is distributed to the tissues. Furthermore, the short half-life (t 1/2 ) of ARB-89 compared to artemisinin [21] also indicates that the compound is removed rapidly from the blood. Oral bioavailability of ARB-89 was found to be 23.4%.…”

Section: Application To Pharmacokinetic Studymentioning

confidence: 99%