Simultaneous determination of 6-gingerol, 8-gingerol, 10-gingerol and 6-shogaol in rat plasma by liquid chromatography–mass spectrometry: Application to pharmacokinetics

Wang, Wei; Li, Chang-Yin; Wen, Xin; Li, Ping; Qi, Lian‐Wen

doi:10.1016/j.jchromb.2009.01.021

Cited by 79 publications

(81 citation statements)

References 24 publications

(21 reference statements)

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“…Along with our collaborators, we have reported that [6]-shogaol was more effective than [6]-gingerol in inhibiting 12-O-tetradecanoylphorbol-13-acetate-induced tumor promotion in mice (Wu et al, 2010). Furthermore, Dugasani et al (2010) The pharmacokinetics of [6]-shogaol in mice and in humans have been investigated (Zick et al, 2008(Zick et al, , 2010Wang et al, 2009;Asami et al, 2010;Iwabu et al, 2010). Yu et al (2007) reported that free [6]-shogaol and glucuronidated and sulfated metabolites of [6]-shogaol were detected in the plasma with peak concentrations of 13.6 Ϯ 6.9 ng/ml, 0.73 Ϯ 0.54 g/ml, and 0.047 Ϯ 0.035 g/ml, respectively, 1 h after oral administration of 2.0 g of ginger extracts (containing 45.04 mg of [6]-shogaol) in humans.…”

Section: Introductionmentioning

confidence: 97%

“…), a member of the Zingiberaceae family, has been cultivated for thousands of years as a spice and for medicinal purposes. Ginger has received extensive attention because of its antioxidant, anti-inflammatory, and anticancer activities (Kawai et al, 1994;Surh, 2002;Shukla and Singh, 2007;Zick et al, 2008;Wang et al, 2009). The major pharmacologically active components of ginger are gingerols and shogaols (Masada et al, 1974;Jiang et al, 2005Jiang et al, , 2006Jiang et al, , 2007Yu et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Metabolism of [6]-Shogaol in Mice and in Cancer Cells

Chen

Lv²,

Soroka³

et al. 2012

Drug Metab Dispos

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ABSTRACT:Ginger has received extensive attention because of its antioxidant, antiinflammatory, and antitumor activities. However, the metabolic fate of its major components is still unclear. In the present study, the metabolism of [6]-shogaol, one of the major active components in ginger, was examined for the first time in mice and in cancer cells. Thirteen metabolites were detected and identified, seven of which were purified from fecal samples collected from

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Metabolism of [6]-Shogaol in Mice and in Cancer Cells

Chen

Lv²,

Soroka³

et al. 2012

Drug Metab Dispos

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“…The antioxidative, anti-inflammatory, and antitumor properties of ginger (Zingiber officinale Roscoe, Zingiberaceae) have been reported in previous studies (1)(2)(3)(4)(5). Various clinical trials have evaluated ginger extracts to decrease lipid levels (6), treat arthritis (7,8), prevent nausea and vomiting (9)(10)(11), and reduce pain in women with primary dysmenorrhea (12).…”

Section: Introductionmentioning

confidence: 99%

Examination of the Pharmacokinetics of Active Ingredients of Ginger in Humans

Zick

et al. 2011

AAPS J

106

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Abstract. Ginger extracts have been studied in various clinical trials for different indications. However, the pharmacokinetics of the ginger active constituents in human biological matrices is not well investigated. This study aims to develop a LC-MS/MS method for simultaneous measurement of 6-, 8-, and 10-gingerols and 6-shogaol and study their pharmacokinetics in human plasma and colon tissues. A sensitive LC-MS/MS method was established and validated with a low limit of quantification of 2-5 ng/ mL. The intra-and inter-day accuracy ranged from −7.3% to 10.4% and from −9.4% to 9.8%, respectively. The intra-and inter-day precision ranged from 0.9% to 10.9% and from 2.0% to 12.4%, respectively. The glucuronide and sulfate metabolites of 6-, 8-, and 10-gingerols and 6-shogaol in plasma and colon tissues were quantified after hydrolysis with β-glucuronidase and sulfatase. After oral dosing of 2.0 g ginger extracts in human, free 10-gingerol and 6-shogaol were detected in plasma with peak concentrations (9.5±2.2 and 13.6±6.9 ng/mL, respectively) at 1 h after oral administration, but no free 6-gingerol and 8-gingerol were detected in plasma from 0.25 to 24 h. The peak concentrations of glucuronide metabolites of 6-, 8-, and 10-gingerols and 6-shogaol were 0.47±0.31, 0.17±0.14, 0.37±0.19, and 0.73±0.54 μg/mL at 1 h, respectively. The peak concentrations of the sulfate metabolites of 6-, 8-, and 10-gingerols and 6-shogaol were 0.28±0.15, 0.027±0.018, 0.018±0.006, and 0.047±0.035 μg/mL at 1 h, respectively. Very low concentrations (2-3 ng/mL) of 10-gingerol glucuronide and sulfate were found in colon tissues. Pharmacokinetic analysis showed that half-lives of these four analytes and their metabolites were 1-3 h in human plasma. No accumulation was observed for 6-, 8-, and 10-gingerols and 6-shogaol and their metabolites in both plasma and colon tissues after multiple daily dosing.

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“…It has been used since antiquity for its health benefits (13). Extracts obtained from its roots usually contain polyphenol compounds, such as [6]-gingerol, [8]gingerol, and [10]-gingerol, which have been cited as the main components responsible for its pharmacological effects (14,15). Among other potential mechanisms, ginger has antioxidant (15)(16)(17) and anti-inflammatory properties (15,18,19).…”

mentioning

confidence: 99%

Gingerol Fraction from Zingiber officinale Protects against Gentamicin-Induced Nephrotoxicity

Rodrigues

Prata

Oliveira

et al. 2014

Antimicrob Agents Chemother

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. Histopathological findings of tubular degeneration and inflammatory cell infiltration reinforced GM-induced nephrotoxicity. All these alterations were attenuated by previous oral treatment with GF. Animals from the GM؉GF groups showed amelioration in renal function parameters and reduced lipid peroxidation and nitrosative stress, in addition to an increment in the levels of glutathione (GSH) and superoxide dismutase (SOD) activity. Gingerols also promoted significant reductions in mRNA transcription for TNF-␣, IL-2, and IFN-␥. These effects were dose dependent. These results demonstrate that GF promotes a nephroprotective effect on GM-mediated nephropathy by oxidative stress, inflammatory processes, and renal dysfunction.

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Simultaneous determination of 6-gingerol, 8-gingerol, 10-gingerol and 6-shogaol in rat plasma by liquid chromatography–mass spectrometry: Application to pharmacokinetics

Cited by 79 publications

References 24 publications

Metabolism of [6]-Shogaol in Mice and in Cancer Cells

Metabolism of [6]-Shogaol in Mice and in Cancer Cells

Examination of the Pharmacokinetics of Active Ingredients of Ginger in Humans

Gingerol Fraction from Zingiber officinale Protects against Gentamicin-Induced Nephrotoxicity

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