Über die Induktionsfähigkeit von Microsomen- und Zellkernfraktionen aus Embryonen und Leber von Hühnern

Kesselring, Kurt; Becker, Ursula; Tiedemann, Heinz

doi:10.1016/0012-1606(62)90041-6

Cited by 24 publications

(2 citation statements)

References 26 publications

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“…The fact that, as shown by Brachet et al (1952) and, more clearly, by Yamada (1961), isolated microsomes have a strong neural inducing activity, also suggests that ribonucleoproteins are closely associated with neural induction. Further analysis of the inducing activity of microsomes isolated from chick embryos (Kawakami et al, 1961;Tiedemann, et al, 1962) has shown that the ribosome fraction which contains only RNA and associated proteins (with a basic protein as the main constituent) has a strong neural inducing activity. On the other hand, the whole microsomes give spino-caudal inductions: the spino-caudal (mesodermal) factor is thus presumably associated with the membrane component of the endoplasmic reticulum.…”

Section: Rna Distribution During Developmentmentioning

confidence: 99%

The Role of the Nucleic Acids in the Processes of Induction, Regulation and Differentiation in the Amphibian Embryo and the Unicellular Alga, Acetabularia Mediterranea

Brachet

1963

Biological Organization at the Cellular and Supercellular Level

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The rôle of nucleic acids (DNA and RNA) during cleavage and differentiation in amphibian eggs is discussed, with spécial emphasis on the mechanisms of DNA sjTithesis in normal embryos and lethal hybrids, and on the RNA distribution during development. The importance of the cell nucleus for RNA and protein synthesis in the unicellular alga Acetabularia mediterranea is discussed on the basis of récent experiments: it is suggested that ribosomal RNA lies xmder a much doser nuclear control than transfer and chloroplastic RNA's. A hypothesis oonoeming the rôle of messenger and ribosomal RNA in morphogenesis is presented.

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Section: Rna Distribution During Developmentmentioning

confidence: 99%

The Role of the Nucleic Acids in the Processes of Induction, Regulation and Differentiation in the Amphibian Embryo and the Unicellular Alga, Acetabularia Mediterranea

Brachet

1963

Biological Organization at the Cellular and Supercellular Level

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“…The factor is called mesodermalizing factor. In chicken muscle homogenate it is preferentially bound together with a neuralizing factor to particles which can be sedimented at 105,000 X g [2] . The supernatant has almost no inducing activity.…”

Section: Introductionmentioning

confidence: 99%

Activation of a morphogenetic factor by electrophoresis

Born

Tiedemann

1969

FEBS Letters

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Effects of nutrient media on gastrula ectoderm of Rana pipiens cultured in vitro

Möser

Flickinger

1963

J. Exp. Zool.

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It has been suggested that the molecular basis of the induction of cellular differentiation might depend upon the provision of materials which stimulate protein synthesis in the reacting cells (Flickinger, '58, '60, '62). One way to test this speculation is to culture explants of undifferentiated embryonic cells in the presence of substrates and cofactors which are necessary for protein synthesis to ascertain if they will stimulate differentiation. The present report deals with experiments in which explants of the ventral mesoderm-ectoderm and ectoderm alone of the early gastrula of the frog embryo, Rana pipiens, were cultured in various nutrient media. MATERIALS AND METHODSVentral mesoderm-ectoderm and ectoderm areas were isolated from stage 10 gastrulae ( fig. 1) and were cultured on millipore filter assemblies ( fig. 2), according to the method of Grobstein ('56), or in plastic petri dishes. The explants were cultured in a CO, incubator (5% C0295% air) at a temperature of 23"C, under humid conditions, for a period of eight days. The cultures were examined daily and were processed for histological examination at the termination of the culture period. The medium used in the control series of experiments was Niu-Twitty solution (Flickinger, '49), which is comprised solely of inorganic salts.The nutrient media used were the amino acid and vitamin mixtures of Eagle's basal medium and NCTC 109 (Paul, '59); the latter medium contains amino acids, vitamins, nucleotides, and coenzymes. Since NCTC 109 was designed for the culture of mammalian cells, it was diluted with an equal volume of deionized water, and each liter was fortified with NaHC03 (1,100 mg) and Na2HP04 (70 mg) so as to maintain a pH of 7.5 during culture in the presence of 5% CO,. A preparation containing 5,000 units each of penicillin-streptomycin was employed at a 1% level to give a final concentration of 50 units/ml of each antibiotic in all the media. The nutrient media were also supplemented with 1% glutamine, 0.2% dextrose, 5 or 7% horse serum, and 1-7% chick embryo extract, and these fractions were used in various combinations in the experimental series. Horse serum, embryo extract, NCTC 109, Eagle's vitamin and amino acid mixtures, glutamine and penicillin-streptomycin were obtained commercially; the embryo extract and horse serum being supplied in a frozen state. The millipore-filter culture dishes were filled with 1.5 ml of medium, while the small, plastic petri dishes were filled with 1.2 ml. The media were changed every two days. In both the petri dishes and the filter assemblies the explant was covered by a very thin film of fluid, but in the latter case the tissues were also exposed to culture fluid diffusing up through the millipore filter.

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Über die Induktionsfähigkeit von Microsomen- und Zellkernfraktionen aus Embryonen und Leber von Hühnern

Cited by 24 publications

References 26 publications

The Role of the Nucleic Acids in the Processes of Induction, Regulation and Differentiation in the Amphibian Embryo and the Unicellular Alga, Acetabularia Mediterranea

The Role of the Nucleic Acids in the Processes of Induction, Regulation and Differentiation in the Amphibian Embryo and the Unicellular Alga, Acetabularia Mediterranea

Activation of a morphogenetic factor by electrophoresis

Effects of nutrient media on gastrula ectoderm of Rana pipiens cultured in vitro

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