Tyrosinase Inhibition, 2,2-Diphenyl-1-Picrylhydrazyl Radical Scavenging Activity, and Phytochemical Screening of Fractions and Ethanol Extract From Leaves and Stem Bark of Matoa (Pometia Pinnata)

Sauriasari, Rani; Azizah, Nur; Basah, Katrin

doi:10.22159/ajpcr.2017.v10s5.23105

Cited by 7 publications

(7 citation statements)

References 17 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, quercetin glucoside, with an IC 50 value of 1.9 mM, inhibited tyrosinase more effectively than the positive standard, kojic acid. [173][174][175][176][177][178] Jamshaid et al, 85 synthesized some 3-hydroxyavone derivatives and tested their TI potential against mushroom tyrosinase (Table 13). Fascinatingly, the test analogs 2-(3- on the ability of a 'S' atom to coordinate with the dinuclear Cu 2+ in the enzyme's active site.…”

Section: Flavonolsmentioning

confidence: 99%

Natural and synthetic flavonoid derivatives as new potential tyrosinase inhibitors: a systematic review

et al. 2021

View full text Add to dashboard Cite

show abstract

Section: Flavonolsmentioning

confidence: 99%

Natural and synthetic flavonoid derivatives as new potential tyrosinase inhibitors: a systematic review

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Extracts underwent phytochemical analysis to determine the presence of alkaloids, flavonoids, tannins, saponins, quinones, and triterpenoids according to common method performed by Farnsworth [2,14].…”

Section: Qualitative Phytochemical Analysismentioning

confidence: 99%

Arginase Inhibitory Activity of Stem Bark Extracts of Caesalpinia Tortuosa Roxb

2018

View full text Add to dashboard Cite

Objective: This study aimed to evaluate the arginase inhibitory activity of Caesalpinia tortuosa Roxb. stem bark extracts.Methods: C. tortuosa Roxb. stem bark extracts were obtained through reflux extraction using n-hexane, ethyl acetate, and methanol and their inhibitory activity against arginase was measured using a microplate reader at 430 nm. Active extracts were subjected to phytochemical analysis and based on the qualitative phytochemical analysis, quantitative data regarding flavonoid and phenolic contents were obtained. The total flavonoid content of active extracts was determined using AlCl 3 colorimetric method, and the phenolic content was determined using Folin-Ciocalteu method. Results:Ethyl acetate and methanol extracts of C. tortuosa Roxb. inhibited activity of arginase with IC 50 values of 33.81 and 11.58 μg/mL, respectively, nor-NOHA acetate as standard drug inhibited arginase with IC 50 values of I3.77 μg/mL. Both active extracts contained saponins, tannins, and flavonoids. Ethyl acetate and methanol extracts showed a total flavonoid content of 7.41 mgQE/g and 5.05 mgQE/g and total phenolic content of 27.55 mgGE/g and 17.16 mgGE/g, respectively. Methanol extracts had a higher inhibitory activity than ethyl acetate extracts despite having flavonoid and phenolic content, thereby suggesting no correlation between arginase inhibitory activity and flavonoid or phenolic content. Conclusion:Ethyl acetate and methanolic extracts of C. tortuosa Roxb. stem barks containing flavonoids, tannins, and saponins displayed arginase inhibitory activity, and no correlation was observed between arginase inhibitory activity and flavonoid and phenolic content.Substrate optimization was performed using concentrations of 130, 570, 650, and 820 mM as suggested by the protocol. Substrate concentrations were tested using 1 U/mL of arginase enzyme. The procedure was performed in triplicate using 10 µL of bidistillation water, 15 µl of enzyme solution, and 20 µl of substrate solution followed by incubation for 30 min at 37°C. After incubation, 100 µl of urea was directly added, followed by incubation at room temperature (25°C).

show abstract

“…Kojic acid is produced biologically by various microorganisms, including species of Aspergillus and Penicillium, and involves aerobic fermentation using various substrates. Kojic acid-producing microorganisms, such as Aspergillus oryzae, Aspergillus flavus, Aspergillus tamarii, and Aspergillus parasiticus are reported to produce high levels of kojic acid [1][2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Aspergillus Oryzae Fermentation Conditions for Kojic Acid Production

2018

View full text Add to dashboard Cite

Objective: The objective of this study is to obtain the optimal fermentation conditions for Aspergillus oryzae by gradually optimizing the medium andfermentation conditions.Methods: Fermentation condition were optimized by varying carbon and nitrogen sources, pH medium, temperature, and aeration condition. Levelsof kojic acid were determined using thin-layer chromatography-densitometry with an ultraviolet detector at a wavelength of 318 nm.Results: A combination of sucrose and yeast extract was chosen as the best source of carbon and nitrogen, respectively, from nine medium variations,producing 1.5425 g/L kojic acid. The optimum acidity of the medium was at pH 4.5, producing 1.7127 g/L of kojic acid, compared with pH 3.5 and5.5. Compared with fermentation at room temperature, fermentation at 35°C yielded greater kojic acid production. The best aeration condition was100 mL medium in 250 mL flask that produced the most kojic acid (1.6472 g/L).Conclusion: The optimal medium for fermentation of kojic acid using A. oryzae uses sucrose and yeast extract as carbon and nitrogen sources,respectively, at pH 4.5. The optimal aeration and incubation conditions were used, a volume of 100 mL in a 250 mL conical flask, incubated at 35°C,obtaining the highest yield value of 0.0370 g/g.

show abstract

Tyrosinase Inhibition, 2,2-Diphenyl-1-Picrylhydrazyl Radical Scavenging Activity, and Phytochemical Screening of Fractions and Ethanol Extract From Leaves and Stem Bark of Matoa (Pometia Pinnata)

Cited by 7 publications

References 17 publications

Natural and synthetic flavonoid derivatives as new potential tyrosinase inhibitors: a systematic review

Natural and synthetic flavonoid derivatives as new potential tyrosinase inhibitors: a systematic review

Arginase Inhibitory Activity of Stem Bark Extracts of Caesalpinia Tortuosa Roxb

Optimization of Aspergillus Oryzae Fermentation Conditions for Kojic Acid Production

Contact Info

Product

Resources

About