Type 1, P and S fimbriae, and afimbrial adhesin I are not essential for uropathogenic<i>Escherichia coli</i>to adhere to and invade bladder epithelial cells

Miyazaki, Jun; Ba-Thein, William; Kumao, Toshio; Yasuoka, Mana; Akaza, Hideyuki; Hayshi, Hideo

doi:10.1111/j.1574-695x.2002.tb00567.x

Cited by 42 publications

(10 citation statements)

References 25 publications

(28 reference statements)

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“…5). It should be also noted that the absence of fimG/H, papG or sfa did not definitely limit the biofilm formation of the studied E. coli strains, which is consist with the results presented by Miyazaki (2002). Therefore, the presence of others genes encoding adhesins is highly possible.…”

Section: Presence Of Genes Associated With Biofilm Formationsupporting

confidence: 80%

Analysis of uropathogenic Escherichia coli biofilm formation under different growth conditions

2015

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The ability to form different types of biofilm enables bacteria to survive in a harsh or toxic environment. Different structures of biofilms are related to different surfaces and environment of bacterial growth. The aim of this study was analysis of the biofilm formation of 115 clinical uropathogenic Escherichia coli strains under different growth conditions: surface for biofilm formation, medium composition and time of incubation. The biofilm formation after 24 h, 48 h, 72 h and 96 h was determined spectrophotometrically (A531) after crystal violet staining and it was correlated with bacterial growth (A600). The live and dead cells in biofilm structures was also observed on the glass surface by an epi-fluorescence microscope. Additionally, the presence of rpoS, sdiA and rscA genes was analyzed. The statistical significance was estimated by paired T-test. The observed biofilms were different for each particular strain. The biofilm formation was the highest in the rich medium (LB) after 24 h and its level hasn't changed in time. When biofilm level was compared to bacterial growth (relative biofilm) - it was higher in a minimal medium in comparison to enriched medium. These results suggest that most of the bacterial cells prefer to live in a biofilm community under the difficult environmental conditions. Moreover, biofilm formation on polyurethane surface did not correlate with biofilm formation on glass. It suggests that mechanisms of biofilm formation can be correlated with other bacterial properties. This phenomenon may explain different types of biofilm formation among one species and even one pathotype - uropathogenic Escherichia coli.

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Section: Presence Of Genes Associated With Biofilm Formationsupporting

confidence: 80%

Analysis of uropathogenic Escherichia coli biofilm formation under different growth conditions

2015

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“…The ExPEC prototype strains CFT073, 536, J96, and RS218 belong to phylogroup B2, and all showed the phenotypic capacity to adhere to and invade a variety of epithelial cells, survival the complement bactericidal activity, and produce biofilms [18][19][20][21][22][23][24][25]. Nevertheless, these features were expressed at different levels in clinical isolates [22,26,27]. Moreover, most of the information related to virulence properties expression assessed in vitro were derived from strains from phylogroups B2 and D [12,24].…”

Section: Introductionmentioning

confidence: 99%

Virulence Potential of a Multidrug-Resistant Escherichia coli Strain Belonging to the Emerging Clonal Group ST101-B1 Isolated from Bloodstream Infection

et al. 2020

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Escherichia coli EC121 is a multidrug-resistant (MDR) strain isolated from a bloodstream infection of an inpatient with persistent gastroenteritis and T-zone lymphoma that died due to septic shock. Despite causing an extraintestinal infection, previous studies showed that it did not have the usual characteristics of an extraintestinal pathogenic E. coli. Instead, it belonged to phylogenetic group B1 and harbored few known virulence genes. To evaluate the pathogenic potential of strain EC121, an extensive genome sequencing and in vitro characterization of various pathogenicity-associated properties were performed. The genomic analysis showed that strain EC121 harbors more than 50 complete virulence genetic clusters. It also displays the capacity to adhere to a variety of epithelial cell lineages and invade T24 bladder cells, as well as the ability to form biofilms on abiotic surfaces, and survive the bactericidal serum complement activity. Additionally, EC121 was shown to be virulent in the Galleria mellonella model. Furthermore, EC121 is an MDR strain harboring 14 antimicrobial resistance genes, including blaCTX-M-2. Completing the scenario, it belongs to serotype O154:H25 and to sequence type 101-B1, which has been epidemiologically linked to extraintestinal infections as well as to antimicrobial resistance spread. This study with E. coli strain EC121 shows that clinical isolates considered opportunistic might be true pathogens that go underestimated.

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“…Type I pili are found in uropathogenic as well as human commensal E. coli strains (Levine et al, 1980). UPEC adheres even without the expression of either of these two pili, suggesting that an alternative mechanism is involved in adhesion (Miyazaki et al, 2002). Recent studies have shown the importance of yad genes in adherence, as the deletion of yad genes increases cell motility.…”

Section: Introductionmentioning

confidence: 99%

Cloning, purification, crystallization and preliminary X-ray diffraction studies ofEscherichia coliPapD-like protein (EcpD)

et al. 2012

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Many Gram-negative bacteria are characterized by hair-like proteinaceous appendages on their surface known as fimbriae. In uropathogenic strains of Escherichia coli, fimbriae mediate attachment by binding to receptors on the host cell, often contributing to virulence and disease. E. coli PapD-like protein (EcpD) is a periplasmic chaperone that plays an important role in the proper folding and guiding of Yad fimbrial proteins to the outer membrane usher protein in a process known as pilus biogenesis. EcpD is essential for pilus biogenesis in uropathogenic E. coli and plays an important role in virulence. In the present study, EcpD was cloned, overexpressed, purified and crystallized by the hanging-drop vapour-diffusion method. The crystals diffracted to 1.67 Å resolution and belonged to the orthorhombic space group C222 1 , with unit-cell parameters a = 100.3, b = 127.6, c = 45.9 Å . There was a single molecule in the asymmetric unit and the corresponding Matthews coefficient was calculated to be 3.02 Å 3 Da À1 , with 59% solvent content. Initial phases were determined by molecular replacement.

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Type 1, P and S fimbriae, and afimbrial adhesin I are not essential for uropathogenicEscherichia colito adhere to and invade bladder epithelial cells

Cited by 42 publications

References 25 publications

Analysis of uropathogenic Escherichia coli biofilm formation under different growth conditions

Analysis of uropathogenic Escherichia coli biofilm formation under different growth conditions

Virulence Potential of a Multidrug-Resistant Escherichia coli Strain Belonging to the Emerging Clonal Group ST101-B1 Isolated from Bloodstream Infection

Cloning, purification, crystallization and preliminary X-ray diffraction studies ofEscherichia coliPapD-like protein (EcpD)

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