Türkiye’deki Yerli Sığır Irklarında Leptin Geni Polimorfizmleri

SummaryCalpastatin (CAST) is a specific inhibitor of calcium-dependent neutral protease μ-calpain found in mammalian tissues. The genetic variants in the bovine CAST gene were analysed by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The animal material of the study consisted of 132 bulls and heifers in Turkish Grey steppe cattle and Turkish Grey steppe x Brown Swiss crossbreds. C and G alleles are characterized by CAST/RsaI polymorphism were detected in the sample of animals were studied. Allele frequency C was significantly frequent in the crossbred group compared to the pure Turkish Grey steppe animals (P<0.05). In the total samples of animals, the average allele frequency C was 56.1%. Genotypic frequencies were estimated as 0.257, 0.499 and 0.243 in the purebred Turkish Grey steppe, and 0.388, 0.470 and 0.142 in the Turkish Grey steppe x Brown Swiss crossbred population for genotypes CC, CG and GG, respectively. As a result, genotypic distributions were equilibrium in both pure and crossbred examples of Turkish Grey steppe cattle.

Section: Introductionmentioning

confidence: 99%

Characterization of Calpastatin Gene in Purebred and Crossbred Turkish Grey Steppe Cattle

Kök¹,

Atalay²,

Savaşçi³

et al. 2013

“…Investigation on the Polymorphism ... polymorphic sites within the target region, more than two alleles can be found [27][28][29] .…”

mentioning

confidence: 99%

Investigation on the Polymorphism of Some Loci by Using PCR-RFLP in Japanese Quails (Coturnix coturnix japonica) Raised in Different Locations of Turkey

Bozkaya¹,

Gürler²,

Yertürk³

2013

The aim of this study was to investigate the restriction fragment length polymorphism (RFLP) of some loci on chromosomes 1 (CJA1) and 3 (CJA3) in Japanese quails (Coturnix coturnix japonica) raised in Turkey. With this study population genetics parameters were estimated in order to select individuals for establishing a reference population, which would be used for studies on recombination frequency. Fertilized eggs obtained from flocks raised in six different provinces of Turkey were incubated for 120 h and 191 embryos were collected. From the tissue samples of the embryos, DNA was isolated by using DNA isolation kits. Particular regions of SEMA3E, IFR1, HAL, LOC396025, UGP2, LOC396192, TLX and BMP5 loci were amplified with specifically designed primers for each locus by using PCR technique. The PCR products were cut with an appropriate restriction enzyme for each locus and analysed by using agarose gel electrophoresis. Presence of different alleles, allele and genotype frequencies, heterozygosities and genetic distances were estimated. Out of the eight loci studied, polymorphism was found for the SEMA3E and TLX loci on 1. and 3. chromosomes, respectively, while five loci were found to be monomorphic and one locus (HAL) could not be amplified by PCR. The populations studied were found to be mostly in Hardy-Weinberg equilibrium. The results indicated that the SEMA3E and TLX loci can be used for studying recombination frequencies in the populations included into the study. Keywords: Japanese quail, RFLP, Polymorphism, RecombinationTürkiye'deki Japon Bıldırcınlarında (Coturnix coturnix japonica) Bazı Lokuslardaki Polimorfizmin PCR-RFLP Yöntemi İle Araştırılması ÖzetBu çalışmanın amacı Japon bıldırcınlarında (Coturnix coturnix japonica) 1. (CJA1) ve 3. (CJA3) kromozom üzerinde bulunan bazı genlerdeki kesim bölgesi polimorfizminin (RFLP) araştırılmasıdır. Bu çalışma ile rekombinasyon oranları ile ilgili araştırma amacıyla kullanılacak bir popülasyonu oluşturacak bireylerin seçimine temel teşkil edecek olan popülasyon genetiği parametrelerinin ortaya konması amaçlanmıştır. Türkiyenin altı farklı ilindeki işletmelerden elde edilen döllü bıldırcın yumurtaları 37°C'de 120 saat süreyle inkube edildikten sonra 191 adet embriyodan doku örneği alınmıştır. Alınan doku örneklerinden özel kitler yardımıyla DNA izolasyonu yapılmıştır. SEMA3E, IFR1, HAL, LOC396025, UGP2, LOC396192, TLX ve BMP5 lokuslarının belirli bölgeleri özel olarak dizayn edilmiş olan primerler yardımıyla ve PCR işlemi ile çoğaltılmıştır. Elde edilen PCR ürünleri uygun bir restririksiyon enzimi ile kesilmiş ve agaroz jel elektroforezi yardımıyla ayrımlanmıştır. İncelenen popülasyonlarda farklı allellerin varlığı, genotip ve allel frekansları, heterozigotluk dereceleri ve genetik mesafeler hesaplanmıştır. Çalışmada kullanılan sekiz lokustan 1. ve 3. kromozomlar üzerinde bulunan SEMA3E ve TLX lokuslarında polimorfizm tespit edilmiş, beş lokus monomorfik olarak bulunmuş bir lokusta (HAL) ise PCR işlemi ile çoğaltma yapılamamıştır. Çalışılan popülasyonların büyük...

“…The genetic polymorphism of milk proteins has great importance in animal breeding and has been made in the subject in recent years due to its relationships with production traits, milk composition and milk quality [1][2][3][4][5][6][7][8] . β-lactoglobulin (β-lg) is the major milk whey protein in ruminants, consists of 162 amino acids and forms stable dimmers in milk.…”

Section: Makale Kodu (Article Code): Kvfd-2011-4476mentioning

confidence: 99%

Türk Yağlı Kuyruklu Koyun Irklarındaki Beta-Laktoglobulin Gen Tipleri

Şahin¹,

Karslı²,

Elmacı³

et al. 2009

SummaryThe genetic polymorphism of the β-lactoglobulin gene was investigated in 182 sheep of Turkish fat-tailed sheep breeds by using PCR-RFLP methods. In the study, two alleles (A and B) and three genotypes (AA, BB and AB) at β-lactoglobulin locus were observed. The most common genotype at the β-lactoglobulin locus was AB. The greatest frequency of allele A was detected in Tuj breed as 0.7188.Results of the present study show that genetic polymorphism exists in the Turkish fat-tailed sheep breed population used in this study. Keywords: Turkish sheep breeds, Genetic polymorphism, β-lactoglobulin gene, PCR-RFLP Türk Yağlı Kuyruklu Koyun Irklarındaki Beta-Laktoglobulin Gen TipleriÖzet β-laktoglobulin geninin genetik polimorfizmi Türkiye yağlı kuyruklu koyun ırklarının 182'sinde PCR-RFLP metodu kullanılarak incelenmiştir. Çalışmada, β-laktoglobulin lokusunun iki alleli (A ve B) ve üç genotipi (AA, AB ve BB) gözlenmiştir. β-laktoglobulin lokusundaki en yaygın genotip AB genotipidir. A allelinin en yüksek frekansı 0.7188 olarak Tuj ırkında belirlenmiştir. Mevcut çalışmanın sonuçları, kullanılan Türk yağlı kuyruklu koyun ırklarında genetik polimorfizmin varlığını göstermiştir.