Tuning DNA–nanoparticle conjugate properties allows modulation of nuclease activity

Abstract

A systematic study of the interactions between nucleases and oligonucleotide-coated gold nanoparticles (spherical nucleic acids, SNAs) demonstrates that the modular units of SNAs can be leveraged to either accelerate or suppress nuclease kinetics.

Plasmonic Cross-Linking Colorimetric PCR for Simple and Sensitive Nucleic Acid Detection

“…As presented in Figure B, when using the fixed concentration of AuNPs-p2/T10, the absorption of the supernatant in the positive control decreased as the number of p1 decreased to around 7491 and increased if the loading number of p1 decreased further. This result was reasonable since higher probe surface coverage can lead to lower hybridization efficiency because of the greater steric effect. − However, although higher hybridization efficiency can be achieved with a much lower probe density, fewer assemblies were formed because of the limited amount of p1 (Figure A). The results show that PMNs-p1/T10 synthesized with a p1/T10 ratio of 2:2 offers the best hybridization efficiency, and thus used for the later experiments.…”

A computational study of the influence of nanoparticle shape on clathrin-mediated endocytosis

“…With the development of nanotechnology, nanoparticles (NPs) have opened new avenues for various biomedical applications such as drug/gene delivery, 1,2 molecular imaging 3,4 and diagnostic purposes. [5][6][7] The process of NP internalization into the cell is very important in most of the biomedical applications. Clathrin-mediated endocytosis (CME) is one of the major internalization pathways for eukaryotic cells.…”

A highly specific and flexible detection assay using collaborated actions of DNA-processing enzymes for identifying multiple gene expression signatures in breast cancer

“…26,27 This enables any target sequence to be detected. (2) In contrast with exonuclease-based probe digestion, some endonucleases (e.g., duplex specific nuclease, 25,28 restriction endonuclease, 29 and FEN1 30 ) can produce discrete fragments with predictable sequences. This makes it possible to utilize the generated fragments to develop continuous signal amplification, which increases the reachable sensitivity of molecular diagnostic assays.…”