A computational study of the influence of nanoparticle shape on clathrin-mediated endocytosis

Abstract

Nanoparticles have been widely used in biomedical applications, such as gene/drug. Among physicochemical properties, shape is a vital design parameter for tuning cell uptake of nanoparticles. However, the regulatory mechanism...

Carriers for hydrophobic drug molecules: lipid-coated hollow mesoporous silica particles, and the influence of shape and size on encapsulation efficiency

“…Additionally, encapsulating ellipsoids and cubic nanocarriers within cell membranes and clathrin structures necessitates significant deformation, requiring more energy, thus making the process less favorable for these shapes compared to spherical nanocarriers. 47–49…”

“…This enhanced efficiency is attributed to the compatibility of spherical shape with natural cellular intake processes, particularly, clathrin-mediated endocytosis, which is significantly influenced by the shape of particles. 47 Spherical nanocarriers, due to their shape, facilitate a more straightforward wrapping by Fig. 6 Comparative analysis of pore size, surface area, and pore volume between nanospheres, nanocubes, and nanoellipsoids: nanospheres show a higher pore size (4 nm) while nanocubes on the other hand exhibited the highest surface area and slightly less pore size than nanospheres.…”

“…Additionally, encapsulating ellipsoids and cubic nanocarriers within cell membranes and clathrin structures necessitates significant deformation, requiring more energy, thus making the process less favorable for these shapes compared to spherical nanocarriers. [47][48][49] This study provides a comprehensive overview of the properties of mesoporous silica nanocarriers and the effect of their distinct morphologies on potential biomedical applications, as outlined in Table 1. Consequently, it concludes that the careful selection and design of mesoporous silica nanocarriers, tailored to their specific morphological features, are imperative for enhancing therapeutic efficacy and achieving superior clinical outcomes for various medicinal applications.…”

“…This enhanced efficiency is attributed to the compatibility of spherical shape with natural cellular intake processes, particularly, clathrinmediated endocytosis, which is significantly influenced by the shape of particles. 47 Spherical nanocarriers, due to their shape, facilitate a more straightforward wrapping by the cell membrane, aided by the self-assembly of clathrins, as opposed to the more complex surfaces and angles of ellipsoids and cubes. The process of clathrin-mediated endocytosis is highly sensitive to the shape of nanocarriers, and the efficiency of clathrin-mediated endocytosis as the shape anisotropy of the nanocarriers increases.…”

“…Additionally, encapsulating ellipsoids and cubic nanocarriers within cell membranes and clathrin structures necessitates significant deformation, requiring more energy, thus making the process less favorable for these shapes compared to spherical nanocarriers. [47][48][49] Figure 12. Cell uptake analysis of different mSiO2 shapes: The bar graphs depict the fold increase in cell uptake for mSiO2 of different shapes (spheres, cubes, and ellipsoids) under two experimental conditions: (A, B) with consistent FITC concentration (0.1µg/mL) for all three nanocarriers after 2 hrs and 4 hrs respectively.…”