Plasmonic PCR utilizing metallic nanoparticles has shown strong advantages compared to commercial thermocycler equipment in terms of cost, size and processing time. However, due to the strong fluorescence quenching, plasmonic...
Simple, low-cost, and accurate nucleic acid assay platforms
hold
great promise for point-of-care (POC) pathogen detection, disease
surveillance, and control. Plasmonic photothermal polymerase chain
reaction (PPT-PCR) is a powerful and efficient nucleic acid amplification
technique, but it lacks a simple and convenient analysis method for
POC applications. Herein, we propose a novel plasmonic cross-linking
colorimetric PCR (PPT-ccPCR) assay by integrating plasmonic magnetic
nanoparticle (PMN)-based PPT-PCR with gold nanoparticle (AuNP)-based
cross-linking colorimetry. AuNPs form assembled structures with the
PMNs in the presence of amplicons and collect in a magnetic field,
resulting in color changes to the supernatant. Target DNA with concentrations
as low as 5 copies/μL can be visually detected within 40 min.
The achieved limit of detection was 1.8 copies/μL based on the
absorption signals. This simple and sensitive strategy needs no expensive
instrumentation and demonstrates high potential for POC detection
while enabling further applications in clinical diagnostics.
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