Plasmonic colorimetric PCR for Rapid molecular diagnostic assays

Jiang, Kunlun; Wu, Jun; Qiu, Yuwei; Go, Yun Young; Ban, Kiwon; Park, Hyun June; Lee, Jung‐Hoon

doi:10.1016/j.snb.2021.129762

Cited by 28 publications

(23 citation statements)

References 38 publications

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“…A binary readout is often used for interpreting colorimetric biosensors (e.g., COVID-19 antigen and serological tests), ,, in which red color indicates positive while no color indicates negative readout, for instance. In colorimetric testing based on the aggregation of nanoparticles, the readout is often binary as well.…”

Section: Resultsmentioning

confidence: 99%

Robust Rules for Optimal Colorimetric Sensing Based on Gold Nanoparticle Aggregation

et al. 2023

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Spurred by outstanding optical properties, chemical stability, and facile bioconjugation, plasmonic metals have become the first-choice materials for optical signal transducers in biosensing. While the design rules for surface-based plasmonic sensors are well-established and commercialized, there is limited knowledge of the design of sensors based on nanoparticle aggregation. The reason is the lack of control over the interparticle distances, number of nanoparticles per cluster, or multiple mutual orientations during aggregation events, blurring the threshold between positive and negative readout. Here we identify the geometrical parameters (size, shape, and interparticle distance) that allow for maximizing the color difference upon nanoparticle clustering. Finding the optimal structural parameters will provide a fast and reliable means of readout, including unaided eye inspection or computer vision.

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Section: Resultsmentioning

confidence: 99%

Robust Rules for Optimal Colorimetric Sensing Based on Gold Nanoparticle Aggregation

et al. 2023

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“…Historically, molecular diagnostics assays polymerase chain reaction (PCR) have played a key role in society in curbing the spread and effects of various infectious diseases through early detection [36,62]. Even though, this technology provides fast and reliable results and do not require any post processing, its limitations are still the expensive machinery, lengthy assay times and sophisticated operational processes that often require a trained personnel [63,64].…”

Section: Plasmonic Nanostructures In Diagnostics 41 Molecular Diagnos...mentioning

confidence: 99%

“…Plasmonic optical properties make them ideal for use in molecular diagnostics because they improve sensitivity, selectivity, efficiency in drug delivery and specificity [66,67]. K. Jiang et al reported the use plasmonic magnetic nanoparticles covered with silica core shell for the detection of DNA ranging from 0.5 ng μL −1 to 3 fg μL −1 within 20 minutes using cPCR [62,67]. This study shows the importance of using plasmonic nanoparticles for the detection of diseases in real time that is acquit of traditional molecular diagnosis limitations such as lengthy assay times.…”

Section: Plasmonic Nanostructures In Diagnostics 41 Molecular Diagnos...mentioning

confidence: 99%

“…This study shows the importance of using plasmonic nanoparticles for the detection of diseases in real time that is acquit of traditional molecular diagnosis limitations such as lengthy assay times. In this study, the plasmonic magnetic nanoparticles were used for their dual function of thermal cycling and magnetic separation and detectable color change [62].…”

Section: Plasmonic Nanostructures In Diagnostics 41 Molecular Diagnos...mentioning

confidence: 99%

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Application of Plasmonic Nanostructures in Molecular Diagnostics and Biosensor Technology: Challenges and Current Developments

Nyembe¹,

Mkhohlakali²,

May³

et al. 2023

Plasmonic Nanostructures - Basic Concepts, Optimization and Applications

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The recent global pandemic caused by Covid-19 enforced the urgent need for accessible, reliable, and accurate point-of-care rapid diagnostics based on plasmonic nanostructures. This is because fast and reliable testing was the key driver in curbing the spread of Covid-19. The traditional methods of diagnostics and biosensors often require expensive infrastructure and highly qualified and trained personnel, which limits their accessibility. These limitations perpetuated the impact of Covid-19 in most countries because of the lack of easily accessible point-of-care rapid diagnostic kits. This review revealed that portable and reliable point-of-care diagnostic kits are very crucial in reaching large populations, especially in underdeveloped and developing countries. This gives perspective to novel point-of-care applications. Furthermore, water quality is a very crucial part of food safety, especially in developing countries faced with water contamination. In this chapter, we explored the various challenges and recent developments in the use of plasmonic nanostructures for application in molecular diagnostics and biosensing for the detection of infectious diseases and common environmental pathogens.

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“…Plasmonic photothermal effects of metallic nanomaterials provide creative inspiration for ultrafast amplification times. ,, For instance, the rapid PCR was achieved using laser-mediated volumetric heating from dispersed nanoparticles − or light-emitting diode (LED)-driven surface heating from large area nanostructures. − The plasmonic PCR often faces either nonspecific adsorption of PCR reagents to the nanomaterials or indirect temperature measurement, which substantially reduces the PCR efficiency . Unlike the nanoparticles, the plasmonic nanostructures effectively isolate the PCR mixtures by thin-film passivation and also simplify optical configuration for photothermal excitation. , However, these methods are still under development to increase the reusability of nanomaterials and the cost-effectiveness for the POC applications …”

mentioning

confidence: 99%

Ultrafast Plasmonic Nucleic Acid Amplification and Real-Time Quantification for Decentralized Molecular Diagnostics

Kang

Jang

et al. 2023

ACS Nano

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Point-of-care real-time reverse-transcription polymerase chain reaction (RT-PCR) facilitates the widespread use of rapid, accurate, and cost-effective near-patient testing that is available to the public. Here, we report ultrafast plasmonic nucleic acid amplification and real-time quantification for decentralized molecular diagnostics. The plasmonic real-time RT-PCR system features an ultrafast plasmonic thermocycler (PTC), a disposable plastic-on-metal (PoM) cartridge, and an ultrathin microlens array fluorescence (MAF) microscope. The PTC provides ultrafast photothermal cycling under white-light-emitting diode illumination and precise temperature monitoring with an integrated resistance temperature detector. The PoM thin film cartridge allows rapid heat transfer as well as complete light blocking from the photothermal excitation source, resulting in real-time and highly efficient PCR quantification. Besides, the MAF microscope exhibits close-up and high-contrast fluorescence microscopic imaging. All of the systems were fully packaged in a palm size for point-of-care testing. The real-time RT-PCR system demonstrates the rapid diagnosis of coronavirus disease-19 RNA virus within 10 min and yields 95.6% of amplification efficiency, 96.6% of classification accuracy for preoperational test, and 91% of total percent agreement for clinical diagnostic test. The ultrafast and compact PCR system can decentralize point-of-care molecular diagnostic testing in primary care and developing countries.

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Plasmonic colorimetric PCR for Rapid molecular diagnostic assays

Cited by 28 publications

References 38 publications

Robust Rules for Optimal Colorimetric Sensing Based on Gold Nanoparticle Aggregation

Robust Rules for Optimal Colorimetric Sensing Based on Gold Nanoparticle Aggregation

Application of Plasmonic Nanostructures in Molecular Diagnostics and Biosensor Technology: Challenges and Current Developments

Ultrafast Plasmonic Nucleic Acid Amplification and Real-Time Quantification for Decentralized Molecular Diagnostics

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