Tuneable nanochannel formation for sample-in/answer-out devices

Shallan, Aliaa I.; Gaudry, Adam J.; Guijt, Rosanne M.; Breadmore, Michael C.

doi:10.1039/c3cc38330j

Cited by 11 publications

(24 citation statements)

References 32 publications

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“…In our device,n anojunctions were formed by dielectric breakdown, [14] the simplest and most cost-effective technique for creating an anojunction between two microfluidic channels.W hile it is normally ac haotic process,w ep reviously demonstrated control over the ion permeability by setting ac urrent limit. [13] Thep recise control of the nanopore size (sub-nm) using ac urrent limit was also reported by another group using thin insulating membranes of silicone nitride. [15] In this work, nanojunctions were created in the 100 mmwide PDMS barriers separating two Vchannels from a50mmwide central channel (Figure 1a).…”

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confidence: 55%

“…Single nanojunctions have been reported for the concentration of DNA, [11] proteins, [12] and the extraction of pharmaceuticals from blood. [13] This is the first report of the use of nanojunctions with different pore sizes for the simultaneous extraction and concentration of target analytes from biological samples that can be seamlessly integrated with electrophoretic separation for on-site TDM.…”

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confidence: 99%

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Electrokinetic Size and Mobility Traps for On‐site Therapeutic Drug Monitoring

2015

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The extraction of target analytes from biological samples is ab ottlenecki na nalysis.Amicrofluidic device featuring an electrokinetic size and mobility trap was formed by two nanojunctions of different pore sizet oe xtract and concentrate analytical targets from complex samples.The trap was seamlessly coupled with electrophoretic separation for quantitative analysis.The device was applied to the analysis of ampicillin levels in blood within 5min and al inear response over the range of 2.5-20 mgmL À1 .T his covers the recommended levels for treating sepsis,acritical condition with 30 to 50 %m ortality and unpredicted drug levels.T he device provides an ew opportunity for on-site therapeutic drug monitoring,w hichs hould enable quick and accurate dosing and may save lives in such critical conditions.

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confidence: 55%

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confidence: 99%

Electrokinetic Size and Mobility Traps for On‐site Therapeutic Drug Monitoring

2015

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“…In our device, nanojunctions were formed by dielectric breakdown,14 the simplest and most cost‐effective technique for creating a nanojunction between two microfluidic channels. While it is normally a chaotic process, we previously demonstrated control over the ion permeability by setting a current limit 13. The precise control of the nanopore size (sub‐nm) using a current limit was also reported by another group using thin insulating membranes of silicone nitride 15.…”

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confidence: 56%

Electrokinetic Size and Mobility Traps for On‐site Therapeutic Drug Monitoring

2015

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The extraction of target analytes from biological samples is a bottleneck in analysis. A microfluidic device featuring an electrokinetic size and mobility trap was formed by two nanojunctions of different pore size to extract and concentrate analytical targets from complex samples. The trap was seamlessly coupled with electrophoretic separation for quantitative analysis. The device was applied to the analysis of ampicillin levels in blood within 5 min and a linear response over the range of 2.5-20 μg mL(-1). This covers the recommended levels for treating sepsis, a critical condition with 30 to 50% mortality and unpredicted drug levels. The device provides a new opportunity for on-site therapeutic drug monitoring, which should enable quick and accurate dosing and may save lives in such critical conditions.

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“…Dielectric breakdown of PDMS is 21 V/μm, above which PDMS starts to fail and develop nanofissures. We developed a method to control the pore size of the nanojunctions formed between two microchannels using slightly higher electric breakdown voltage of 22 V/μm over a 100‐μm gap . Different permeability ranges were achieved by setting a current limit during the breakdown to lower the applied voltage once the nanojunctions are formed.…”

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Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2012–2014)

et al. 2015

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One of the most cited limitations of capillary (and microchip) electrophoresis is the poor sensitivity. This review continues to update this series of biannual reviews, first published in Electrophoresis in 2007, on developments in the field of on-line/in-line concentration methods, covering the period July 2012-July 2014. It includes developments in the field of stacking, covering all methods from field-amplified sample stacking and large-volume sample stacking, through to ITP, dynamic pH junction, and sweeping. Attention is also given to on-line or in-line extraction methods that have been used for electrophoresis.

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Tuneable nanochannel formation for sample-in/answer-out devices

Cited by 11 publications

References 32 publications

Electrokinetic Size and Mobility Traps for On‐site Therapeutic Drug Monitoring

Electrokinetic Size and Mobility Traps for On‐site Therapeutic Drug Monitoring

Electrokinetic Size and Mobility Traps for On‐site Therapeutic Drug Monitoring

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2012–2014)

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