Treatments affecting maturation and germination of American chestnut somatic embryos

Robichaud, Rodney L.; Lessard, Veronica C.; Merkle, S. A.

doi:10.1016/j.jplph.2004.03.003

Cited by 56 publications

(38 citation statements)

References 36 publications

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“…Means in each column followed by different letters are significantly different according to DMRT at P<0.05 1997) and enhances the synthesis of certain metabolites (Deo et al 2010). Earlier, the beneficial role of Lglutamine in the induction of somatic embryogenesis has also been reported in a number of studies (Garin et al 2000;Robichaud et al 2004;Zouine and Hadrami 2007;Deo et al 2010). In contrast to our observations, insignificant role of L-glutamine in somatic embryogenesis was also reported in Acacia catechu (Rout et al 1995), Prunus incise (Cheong and Pooler 2004), Psidium guajava (Rai et al 2009).…”

Section: Effect Of Amino Acids On the Induction Of Somatic Embryogenesismentioning

confidence: 86%

Induction of somatic embryogenesis in gum arabic tree [Acacia senegal (L.) Willd.]

Rathore

Manoj

Shekhawat

2012

Physiol Mol Biol Plants

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Factors affecting somatic embryogenesis from immature cotyledon of gum arabic tree [Acacia senegal (L.) Willd.] were investigated. Induction of somatic embryogenesis was influenced by plant growth regulator concentrations and addition of amino acids in medium. Best induction of somatic embryogenesis was obtained on MS medium supplemented with 0.45 μM 2, 4-D, 2.32 μM Kin and 15 mM L-glutamine. L-glutamine plays a significant role in the maturation of somatic embryos and most of embryos attained maturity only on L-glutamine (15 mM) containing medium. Maximum percent (75.0±2.5) germination of somatic embryos was recorded on medium containing 0.22 μM BAP.

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Section: Effect Of Amino Acids On the Induction Of Somatic Embryogenesismentioning

confidence: 86%

Induction of somatic embryogenesis in gum arabic tree [Acacia senegal (L.) Willd.]

Rathore

Manoj

Shekhawat

2012

Physiol Mol Biol Plants

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“…The 6.3% of mature embryos developing only shoots could indirectly regenerate plantlets through a micropropagation procedure (Xing et al 1997). By contrast, Robichaud et al (2004) reported that sucrose level (3-7.5%) in the maturation medium had no effect on the germination frequency of American chestnut embryos, suggesting a possible influence of genotype in order to explain the differences obtained regarding previous studies (Carraway and Merkle 1997;Xing et al 1999). …”

Section: Effect Of Carbohydratesmentioning

confidence: 92%

“…Activated charcoal had no positive effect on the germination and plantlet conversion of European chestnut somatic embryos (Corredoira et al 2003a), whereas in American chestnut it was included in both maturation (Carraway and Merkle 1997;Robichaud et al 2004) and germination media (Carraway and Merkle 1997;Xing et al 1999;Robichaud et al 2004). …”

Section: Other Maturation Treatmentsmentioning

confidence: 99%

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Somatic Embryogenesis in Chestnut

Corredoira

Ballester

Vieitez

et al.

Plant Cell Monographs

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Somatic embryogenesis is an important biotechnological tool that demonstrates significant benefits when applied to forest tree species; clonal propagation, cryostorage of valuable germoplasm and genetic transformation are among the most promising of its applications. In this chapter, the state of the art of somatic embryogenesis in chestnut (an important economical tree species of the genus Castanea) is assessed and discussed. The factors affecting the induction (type of explant, growth conditions, mineral media, plant growth regulators), maintenance and multiplication of the embryogenic cultures (through repetitive embryogenesis) and the maturation and conversion into plants of somatic embryos are described. The latest results achieved on the application of the process on both genetic transformation and cryoconservation of chestnut embryogenic lines are also mentioned.

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“…The control of SSE has been previously reported to enter a maturation phase or to accumulate reserve substances in several studies such as Q. ilex (Mauri and Manzanera, 2004), Castanea dentata (Marshall) Borkh. (Robichaud et al, 2004), Q. suber (García-Martín et al, 2005) and J. regia (Vahdati et al, 2008). The decrease in FW and SSE in treatments where ABA was added can be explained by a possible osmotic besides the effect and ABA's capability to inhibit premature germination and control SSE (Bentsink and Koornneef, 2008;Manoj et al, 2008).…”

Section: Somatic Embryo Maturation Assaymentioning

confidence: 99%

Endogenous Quantification of Abscisic Acid and Indole-3-Acetic Acid in Somatic and Zigotic Embryos of Nothofagus alpina (Poepp. & Endl.) Oerst

Riquelme¹,

Leal²,

Carrillo³

et al. 2011

Chilean J. Agric. Res.

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Abscisic acid (ABA) and indole-3-acetic acid (IAA) participate in the propagation of plants by somatic embryogenesis, causing polar structural differentiation of the embryo. The goal of the assay was to compare endogenous levels of ABA and IAA between somatic embryos (SE) and zygotic embryos (ZE) of Nothofagus alpina (Poepp. & Endl.) Oerst. In this study, a somatic embryo maturation assay involving the addition of varying concentrations of exogenous ABA was performed on cotyledonary-stage of N. alpina. Furthermore, the endogenous levels of ABA and IAA were quantified in the immature ZE, the mature ZE, and the embryonic axis of a mature embryo of N. alpina. The current study utilized high performance liquid chromatography (HPLC) for quantification. The maturation treatments performed did not present significant differences in the endogenous ABA levels in SE. However, significant differences did exist in levels of ABA and IAA between SE submitted to the different maturation treatments and mature ZE of N. alpina. The application of exogenous ABA to the culture medium increased endogenous ABA levels, therefore, increasing the number of germinated somatic embryos. Thus, the plant conversion process was also successfully completed in somatic embryos of N. alpina.

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Treatments affecting maturation and germination of American chestnut somatic embryos

Cited by 56 publications

References 36 publications

Induction of somatic embryogenesis in gum arabic tree [Acacia senegal (L.) Willd.]

Induction of somatic embryogenesis in gum arabic tree [Acacia senegal (L.) Willd.]

Somatic Embryogenesis in Chestnut

Endogenous Quantification of Abscisic Acid and Indole-3-Acetic Acid in Somatic and Zigotic Embryos of Nothofagus alpina (Poepp. & Endl.) Oerst

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