Induction of somatic embryogenesis in gum arabic tree [Acacia senegal (L.) Willd.]

Rathore, Jitendra Singh; Manoj, K.; Shekhawat, N. S.

doi:10.1007/s12298-012-0130-x

Cited by 24 publications

(5 citation statements)

References 28 publications

(35 reference statements)

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“…Rathore et al [51] reported that L-glutamine stimulated the maturation of Acacia senegal somatic embryos and reduced reduced embryo abnormalities. Rai et al [52] found that 0.68 mM of glutamine increased maturation percentage of Psidium guajava somatic embryos.…”

Section: Discussionmentioning

confidence: 99%

Effect of Amino Acids on Secondary Somatic Embryogenesis of Moroccan Cork Oak (<i>Quercus suber</i> L.) Tree

Rahmouni¹,

Ansari²,

Badoc³

et al. 2020

AJPS

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In the present study, we tested the effect of amino acids on secondary somatic embryogenesis of Moroccan cork oak (Quercus suber L.). Secondary mature and immature somatic embryos were obtained from primary somatic embryos cultured in N 30 K medium supplemented with nineteen amino acids. Stimulation of embryogenesis was dependent on the type and concentration of amino acid in the medium. Thus, L-glutamine and L-asparagine at 3.42 mM have been proved to be the most favorable for the formation of functional somatic embryos and the induction of secondary somatic embryos.

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Section: Discussionmentioning

confidence: 99%

Effect of Amino Acids on Secondary Somatic Embryogenesis of Moroccan Cork Oak (<i>Quercus suber</i> L.) Tree

Rahmouni¹,

Ansari²,

Badoc³

et al. 2020

AJPS

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“…(Kong et al, 2012), Acacia senegal (L.) Willd. (Rathore et al, 2012), Cassia angustifolia (Parveen & Shahzad, 2014). These explants are more proficient for somatic embryo induction, metabolically and biochemically, due to presence of milky white content in immature cotyledons of neem (Gairi & Rashid, 2005).…”

Section: Somatic Embryogenesismentioning

confidence: 99%

Regeneration potential of different explants during micropropagation of neem tree (Azadirachta indica A. Juss.)

Dhandapani

Gnanam

Senthil

et al. 2021

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Azadirachta indica A. Juss., (Neem), a prodigious multipurpose tree, has immense potential to benefit mankind and to protect the environment. In order to investigate the effects of three different explants for its regeneration potential, de embryonated cotyledon, immature zygotic embryo and nodal segments from a 30 year old neem plus tree were used. Half strength MS medium with benzyl amino purine (3 mg/L) and naphthalene acetic acid (0.5 mg/L) and casein hydrolysate (1 g/L) was effective in shoot bud sprouting from both nodes and cotyledons. Half strength MS medium fortified with TDZ (0.2 mg/L) was effective for induction of somatic embryogenesis from zygotic embryos. Shoot buds initiated from the cotyledons produced a maximum number of shoots per explants (4.33) which on further sub culturing induced maximum multiple shoots (15) on half strength MS medium fortified with BAP (1.5 mg/L), NAA (0.5 mg/L) and CH (400 g /L) and the nodal explants induced only 4-5 axillary shoots on further sub culturing. Even though immature zygotic embryos produced more number of somatic embryos per explant (24.97) within a short time (30-45 days), the plantlet conversion was poor (25.52 %). In vitro rooting was observed in half strength MS medium supplemented with IBA (2 mg/L). The regeneration potential of de embryonated cotyledons through a simple regeneration system may be beneficial for efficient mass propagation of selected plus trees of neem.

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“…Furthermore, the surface sterilization of A. auriculiformis had many procedures, the explant was soaked in 0.1% Benlate (fungicide) combination with 1% Boric acid for 24 hours before sterilized with 0.1% HgCl2 for 10 minutes (first sterilization) and 10% Clorox ® for 5 minutes (second sterilization). Although, mercuric chloride is highly antimicrobial, with action against both bacteria and fungi, it should be used at 0.05 -0.1% for 5-10 minutes (Rathore et al, 2012;Banerjee, 2013;Monteuuis et al, 2013;Nagashree et al, 2015). The sterilization studies of Acacia sp.…”

Section: Surface Sterilization Techniquementioning

confidence: 99%

In Vitro Propagation of Acacia Hybrid from Axillary Buds

Tongkok¹,

Chusrisom²,

Sukpiboon³

et al. 2021

AAHAE

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Acacia hybrid is a fast-growing tree species which plants in the forest plantation for energy in Southeast Asia, including Thailand. The efficient method for rapid propagation for forest plantation is tissue culture technology. A sterilization is one of the most important steps in tissue culture. The effects of type, concentration, and duration of disinfectants were examined. Axillary bud was used as explant for in vitro propagation. The procedures for sterilization were conducted six treatments and cultured in ½MS for 4 weeks. There was significantly different for all treatments in the percentage of survival, contamination, dead explants, and growth performance (P<0.05). The optimal sterilization procedure was rinsed in running water for 5 minutes +20% dishwashing liquids+70% (v/v) Ethanol for 30 seconds + 0.1% HgCl2 for 10 minutes and rinsing with sterile water for 4 times. The results showed 13.50±9.26% contaminated explants and 86.50±9.26% survived explants. The shoot length, leaf length and leaf number were 3.40±0.20 cm., 5.04±0.70 cm. and 3.3±0.48 no./explant, respectively. Axillary bud from sterilized explant was transferred to MS medium supplemented with 30 g/l sucrose+0.6 mg/l BAP+0.6 mg/l NAA+0.3 mg/l PVP for shoot multiplication. The maximum number of shoots after 8 weeks was 10.5±1.06 shoots/explant. Each microshoot was elongated and rooted on MS basal medium without plant growth regulators.

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Induction of somatic embryogenesis in gum arabic tree [Acacia senegal (L.) Willd.]

Cited by 24 publications

References 28 publications

Effect of Amino Acids on Secondary Somatic Embryogenesis of Moroccan Cork Oak (<i>Quercus suber</i> L.) Tree

Effect of Amino Acids on Secondary Somatic Embryogenesis of Moroccan Cork Oak (<i>Quercus suber</i> L.) Tree

Regeneration potential of different explants during micropropagation of neem tree (Azadirachta indica A. Juss.)

In Vitro Propagation of Acacia Hybrid from Axillary Buds

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Induction of somatic embryogenesis in gum arabic tree [Acacia senegal (L.) Willd.]

Cited by 24 publications

References 28 publications

Effect of Amino Acids on Secondary Somatic Embryogenesis of Moroccan Cork Oak (&lt;i&gt;Quercus suber&lt;/i&gt; L.) Tree

Effect of Amino Acids on Secondary Somatic Embryogenesis of Moroccan Cork Oak (&lt;i&gt;Quercus suber&lt;/i&gt; L.) Tree

Regeneration potential of different explants during micropropagation of neem tree (Azadirachta indica A. Juss.)

In Vitro Propagation of Acacia Hybrid from Axillary Buds

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Effect of Amino Acids on Secondary Somatic Embryogenesis of Moroccan Cork Oak (<i>Quercus suber</i> L.) Tree

Effect of Amino Acids on Secondary Somatic Embryogenesis of Moroccan Cork Oak (<i>Quercus suber</i> L.) Tree