Transplantation of muscle-derived stem cells plus biodegradable fibrin glue restores the urethral sphincter in a pudendal nerve-transected rat model

Xu, Yanfang; Song, Yanfeng; Lin, Ze

doi:10.1590/s0100-879x2010007500112

Cited by 31 publications

(35 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MDSCs tend to differentiate into muscle cells and muscle tissue in the absence of specific differentiation-induction, and differentiate into bone cells, cartilage cells, smooth muscle cells, endothelial cells, and neurons in vitro when specific interventions are performed (Gates et al 2008;Smaldone et al 2009). Therefore, MDSCs are valuable in treating muscular dystrophy (Ambrosio et al 2009), heart disease (Shibuya et al 2010), stress urinary incontinence (Stangel-Wojcikiewicz et al 2010), neurogenic bladder (Nitta et al 2010), bone disease, and central nervous system disease (Matsumoto et al 2009;Xu et al 2010). Nolazco et al (2008) infused MDSCs into penile corpora cavernosa of old rats and observed that they differentiated into smooth muscle cells, resulting in significant improvement of erectile function.…”

Section: Discussionmentioning

confidence: 99%

Isolation and passage of muscle-derived stem cells from the rat penile corpora cavernosa and induction of differentiation into smooth muscle cells

Xue

Dong

et al. 2013

Cytotechnology

View full text Add to dashboard Cite

This study treated the isolation and passage of muscle-derived stem cells (MDSCs) from rat penile corpora cavernosa, detection of stem cell marker expression, observation of their self-renewal and continuous proliferation, and demonstration of their potential to differentiate into smooth muscle cells in co-culture. Muscle-derived stem cells from the rat penile corpora cavernosa were isolated and purified. The expression of stem cell markers Sca-1 and desmin was detected in PP6 cells, thus confirming that the main components of PP6 cells are MDSCs. The expression of Sca-1 and desmin occurred both in PP6 cells and cells at passages 3, 6, and 8, and there was no significant decrease in the expression level with increasing passage number. The growth curves indicated that the cell doubling time was approximately 48 h. The cells entered the stationary phase after approximately 7 days of culture. The proliferative activity of the cells at passage 8 remained unchanged. After 2 days of co-culture with smooth muscle cells, the DAPI-labeled MDSCs tended to exhibit smooth muscle cell morphology and expression of a-SMA was detected. MDSCs exist in the rat penile corpora cavernosa and possess the potential to differentiate into smooth muscle cells. This discovery serves as the basis in view of the potential use of endogenous stem cells for the treatment of erectile dysfunction (ED).

show abstract

Section: Discussionmentioning

confidence: 99%

Isolation and passage of muscle-derived stem cells from the rat penile corpora cavernosa and induction of differentiation into smooth muscle cells

Xue

Dong

et al. 2013

Cytotechnology

View full text Add to dashboard Cite

show abstract

“…The Crede technique, employed in 6 SC-SUI studies [19][20][21]46,49,63], is performed manually on an anesthetized animal by applying an increasing external force on the abdomen over the bladder with the experimenter's index finger. Immediately after a leak is visually observed at the urethral meatus, the experimenter lifts his/her finger off, and the peak intravesical pressure, that is, the LPP, is recorded via an intravesical catheter.…”

Section: Functional Assessmentmentioning

confidence: 99%

Stem Cell Therapy for Stress Urinary Incontinence: A Critical Review

Lin

Lue

2012

Stem Cells and Development

View full text Add to dashboard Cite

“…transplanted MDSCs in combination with biodegradable fibrin glue which improved their biological action [71]. The first clinical trial using MDSCs was carried out at the University of Toronto.…”

Section: Regeneration Of Urinary Sphinctermentioning

confidence: 99%

Adult stem cells derived from skeletal muscle — biology and potential

2013

View full text Add to dashboard Cite

Skeletal muscle contains at least two distinct populations of adult stem cells — satellite cells and multipotent muscle-derived stem cells. Monopotential satellite cells are located under the basal lamina of muscle fibers. They are capable of giving rise only to cells of myogenic lineage, which play an important role in the processes of muscle regeneration. Multipotent muscle-derived stem cells are considered to be predecessors of the satellite cells. Under proper conditions, both in vitro and in vivo, they undergo myogenic, cardiogenic, chondrogenic, osteogenic and adipogenic differentiation. The main purpose of the present article is to summarize current information about adult stem cells derived from skeletal muscle, and to discuss their isolation and in vitro expansion techniques, biological properties, as well as their potential for regenerative medicine.

show abstract

Transplantation of muscle-derived stem cells plus biodegradable fibrin glue restores the urethral sphincter in a pudendal nerve-transected rat model

Cited by 31 publications

References 32 publications

Isolation and passage of muscle-derived stem cells from the rat penile corpora cavernosa and induction of differentiation into smooth muscle cells

Isolation and passage of muscle-derived stem cells from the rat penile corpora cavernosa and induction of differentiation into smooth muscle cells

Stem Cell Therapy for Stress Urinary Incontinence: A Critical Review

Adult stem cells derived from skeletal muscle — biology and potential

Contact Info

Product

Resources

About