Isolation and passage of muscle-derived stem cells from the rat penile corpora cavernosa and induction of differentiation into smooth muscle cells

Abstract

This study treated the isolation and passage of muscle-derived stem cells (MDSCs) from rat penile corpora cavernosa, detection of stem cell marker expression, observation of their self-renewal and continuous proliferation, and demonstration of their potential to differentiate into smooth muscle cells in co-culture. Muscle-derived stem cells from the rat penile corpora cavernosa were isolated and purified. The expression of stem cell markers Sca-1 and desmin was detected in PP6 cells, thus confirming that the main components of PP6 cells are MDSCs. The expression of Sca-1 and desmin occurred both in PP6 cells and cells at passages 3, 6, and 8, and there was no significant decrease in the expression level with increasing passage number. The growth curves indicated that the cell doubling time was approximately 48 h. The cells entered the stationary phase after approximately 7 days of culture. The proliferative activity of the cells at passage 8 remained unchanged. After 2 days of co-culture with smooth muscle cells, the DAPI-labeled MDSCs tended to exhibit smooth muscle cell morphology and expression of a-SMA was detected. MDSCs exist in the rat penile corpora cavernosa and possess the potential to differentiate into smooth muscle cells. This discovery serves as the basis in view of the potential use of endogenous stem cells for the treatment of erectile dysfunction (ED).

Mesenchymal stem cell‐derived exosomes ameliorate erection by reducing oxidative stress damage of corpus cavernosum in a rat model of artery injury

“…It is related to the pluripotency maintaining and regulating the differentiation ability of stem cells . Nolazco G et al reported that the quiescent endogenous stem cell was detected in the corpora cavernosa and proved to have the ability of differentiation after induction in vitro . In our study, we found that the expression of VEGF increased in the treatment group meanwhile the number of OCT4‐positive cells around vascular endothelium increased, suggesting that newly formed vascular endothelial cells might be derived from cavernous endogenous stem cell differentiation.…”

Combination of low-energy shock-wave therapy and bone marrow mesenchymal stem cell transplantation to improve the erectile function of diabetic rats

“…On the other hand, LESWT could enhance stem cell proliferation, differentiation and paracrine 44454647. In addition, the types of stem cells detected around the cavernous vasculature, including perisinusoidal and subtunic penile progenitor cells48 and cavernous smooth muscle-derived stem cells,4950 could be induced to activate. Thus, LESWT induced angiogenesis and created a beneficial situation for the establishment of the stem cell niche.…”