Translationally controlled tumor protein against apoptosis from 2-hydroxy-ethyl methacrylate in human dental pulp cells

Abstract

2-Hydroxy-ethyl methacrylate (HEMA) is a major monomer released from resin-base dental restorative materials. HEMA is cytotoxic to pulp cells and leads to apoptosis. This study examined the effect of Translationally Controlled Tumor Protein (TCTP) against apoptosis from HEMA. TCTP from banana prawn (Penaeus merguiensis) was cloned and the protein was purified. It significantly increased the number of viable of HEMA-treated cells compared to HEMA-treated cells alone. Flow cytometry indicated the addition of TCTP at 10 μg/ml to 8 and 10 mM HEMA decreased the apoptotic cells from 20 to 10%. The proliferative property and anti-apoptotic activity against HEMA was concentration dependent. It was interesting that the added TCTP was not detected inside the cells and the native human TCTP was decreased after treated with HEMA and TCTP (20 μg/ml) + HEMA(10 mM) for 24 h. These results provided preliminary information, which may contribute to the development of less toxic dental materials.

Effect of recombinant vascular endothelial growth factor and translationally controlled tumor protein on 2‑hydroxyethyl methacrylate‑treated pulp cells

“…This study evaluated some biological properties of VEGF-A or VEGF, especially the anticytotoxicity, differentiation and mineralization in pulp cells compared to TCTP. Previous studies have reported that VEGF (3,5) and Pmer-TCTP (15) can promote pulp cell proliferation and differentiation, which were confirmed by the results of this study. The VEGF used in this study is in a homodimer form, which has 165 amino acid residues per monomer.…”

“…TCTP and its anti-apoptotic effect against HEMA-treated pulp cells has previously been reported (15). The mechanism whereby TCTP can survive HEMA-treated pulp cells remains unknown.…”

“…TCTP has many functions, including proliferation, maturation, and antiapoptosis (14). TCTP from Penaeus merguiensis (Pmer-TCTP) has been shown to have an antiapoptotic property against HEMA-treated dental pulp cells (15). A new formula of resin Effect of recombinant vascular endothelial growth factor and translationally controlled tumor protein on 2-hydroxyethyl methacrylate-treated pulp cells modified glass ionomer cement supplemented with TCTP can reduce cell death from residual HEMA release (16).…”

Biological activities of a recombinant fortilin from Fenneropenaeus merguiensis

“…EC5505-15-L), Faculty of Dentistry, Prince of Songkla University. Primary pulp cells were prepared using enzymatic digestion [9]. Briefly, the pulp tissue was minced and digested for 1 h at 37˚C in a solution containing 3 mg/ml of collagenase type I and 4 mg/ml of dispase.…”

“…In dentistry, HEMA is a monomer in resin-based restorative materials that is released during the restoration of dental cavities [9]. A recombinant Glutathione-S-transferase-Fortilin (GST-Fortilin) from Fenneropenaeus merguiensis (Fm-GST-Fortilin) reduced the toxicity to dental pulp cells that caused by 2-Hydroxy-ethyl methacrylate (HEMA) were reported [9]. Fm-GST-Fortilin was also reported to promote osteoblast cell proliferation and differentiation function [10].…”