Effect of novel chitosan-fluoroaluminosilicate resin modified glass ionomer cement supplemented with translationally controlled tumor protein on pulp cells

Abstract

Dental materials that can promote cell proliferation and function is required for regenerative pulp therapy. Resin modified glass ionomer cement (RMGIC), a broadly used liner or restorative material, can cause apoptosis to pulp cells mainly due to HEMA (2-hydroxyethyl methacrylate), the released residual monomer. Recent studies found that chitosan and albumin could promote release of protein in GIC while translationally controlled tumor protein (TCTP) has an anti-apoptotic activity against HEMA. The aim of this study was to examine the effect of chitosan and albumin modified RMGIC (Exp-RMGIC) supplemented with TCTP on pulp cell viability and mineralization. Exp-RMGIC+TCTP was composed of RMGIC powder incorporated with 15 % of chitosan, 5 % albumin and supplemented with TCTP mixed with the same liquid components of RMGIC. The effect of each specimen on pulp cells was examined using the Transwell plate. From the MTT assay, Exp-RMGIC+TCTP had the highest percentages of viable cells (P < 0.05) at both 24 and 74 h. Flow cytometry revealed that, after 24 h, Exp-RMGIC+TCTP gave the lowest percentages of apoptotic cells compared to other groups. There was no difference in alkaline phosphatase (ALP) activity among different formula of the specimens, while cells cultured in media with TCTP had higher ALP activity. Von Kossa staining revealed that RMGIC+TCTP, and Exp-RMGIC+TCTP had higher percentages of calcium deposit area compared to those without TCTP. It was concluded that Exp-RMGIC supplemented with TCTP had less cytotoxicity than RMGIC and can protect cells from apoptosis better than RMGIC supplemented with TCTP.

Effect of recombinant vascular endothelial growth factor and translationally controlled tumor protein on 2‑hydroxyethyl methacrylate‑treated pulp cells

“…The results of ALP activity assay and cell mineralization by the ARS assay confirmed the results of other studies, which showed that TCTP (16) and VEGF (2) can enhance differentiation and mineralization of pulp cells. This was also consistent with qRT-PCR results that both TCTP and VEGF had up regulated ALP expression on day 3 compared to the control.…”

“…TCTP from Penaeus merguiensis (Pmer-TCTP) has been shown to have an antiapoptotic property against HEMA-treated dental pulp cells (15). A new formula of resin Effect of recombinant vascular endothelial growth factor and translationally controlled tumor protein on 2-hydroxyethyl methacrylate-treated pulp cells modified glass ionomer cement supplemented with TCTP can reduce cell death from residual HEMA release (16). The aim of this study was to compare cell proliferation, anticytotoxicity, differentiation and mineralization of VEGF-A with TCTP on HEMA-treated human dental pulp cells (HDPCs).…”

Evaluation of push-out bond strength of different concentrations of chitosan nanoparticles incorporated composite resin and eighth-generation bonding agent for class II restoration: An in vitro study

“…Studies have been done evaluating the incorporation of CSN into different restorative materials such as resin-modified cement and glass-ionomer cement. [ 16 ] In the present study, improvements in the composite restoration have been attempted by the addition of CSN to DBA and also in composite resin for class II cavities.…”

Chitosan in different concentrations added to a two-step etch-and-rinse adhesive system: influence on bond strength to dentin

“…Different studies evaluated the inclusion of chitosan into different restorative materials, as resin cement modified [24] and glass ionomer cements [25]. When included into adhesive systems, it was demonstrated that the inclusion of low concentrations of chitosan (0.12 and 0.25%) do not compromise of bond strength to dentin compared to a higher concentration (1%) [21].…”