Topical application of lyophilized and powdered human amniotic membrane promotes diabetic ulcer healing

Rodella, Anna; Pozzobon, Michela; Rigon, Matteo; Franchin, Cinzia; Arrigoni, Giorgio; Simonato, Manuela; Ghinelli, Emiliano; Vedovelli, Luca

doi:10.1016/j.wndm.2019.100171

Cited by 4 publications

(2 citation statements)

References 42 publications

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“…While it has been demonstrated that cryopreservation of the EAM can conserve the cellular viability of the tissue, 26 yet degradation of beneficial proteins associated with long‐term storage is also reported 27 . Commercially available dehydrated amniotic membrane offers the advantage of requiring no specialized cryo‐storage, and it has been reported to improve corneal ulcer healing in diabetic humans 28 ; however, in a recent study, dehydrated EAM did not improve healing for horses with experimentally induced superficial corneal ulcers 29 . The latter results could be due to the preparation method degrading beneficial proteins.…”

Section: Introductionmentioning

confidence: 99%

Determining MMP‐2 and MMP‐9 reductive activities of bovine and equine amniotic membranes homogenates using fluorescence resonance energy transfer

Silva

Gibson

Jeong

et al. 2021

Veterinary Ophthalmology

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Section: Introductionmentioning

confidence: 99%

Determining MMP‐2 and MMP‐9 reductive activities of bovine and equine amniotic membranes homogenates using fluorescence resonance energy transfer

Silva

Gibson

Jeong

et al. 2021

Veterinary Ophthalmology

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“…Studies have revealed that could repair damaged tissue by inhibition of inflammatory [44,45]. Besides, Rodella et al reported that lyophilized and powdered extract of AM ameliorates inflammatory response in chronic diabetic ulcer [46].…”

Section: Discussionmentioning

confidence: 99%

Amnion membrane proteins attenuate LPS-induced inflammation and apoptosis by inhibiting TLR4/NF-κB pathway and repressing MicroRNA-155 in rat H9c2 cells

Akseh

Nemati

Zamani-Gharehchamani

et al. 2021

Immunopharmacology and Immunotoxicology

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Objective: Amnion membrane (AM) has been popular for the treatment of inflammatory disorders due to its cell repairing properties. This current study aims to find the underlying mechanisms of amnion membrane proteins (AMPs) against the pro-inflammatory miRNA, miR-155, miR-146, and antiapoptotic microRNA, miR-21, in LPS-treated H9c2 cells. Methods: Cell viability and apoptosis were determined by MTT assay and annexin V/PI staining. The production of the cytokines, TNF-a and IL-6 were evaluated by using qPCR and Enzyme-linked immunosorbent assay (ELISA), respectively. In addition, the expression of miRNAs was quantified by qPCR, and also the protein level of TLR4 and NF-kb was determined with western blotting. Results: We found that AMPs ameliorated LPS-induced reduction of cell viability and augment apoptosis in H9c2 cells. AMPs efficiently inhibited cytokine expression (IL-6 and TNF-a) and activity of TLR4/ NF-jB pathway in LPS-treated H9c2 cells. Correspondingly, in parallel with the suppression of proinflammatory cytokines and apoptosis, AMPs mitigated pro-inflammatory miRNA, miR-155 expression, while, the expression of miR-155 was found to be increased in LPS-treated H9c2 cells. Also, AMPs activated miR-146 expression in H9c2 cells under LPS treatment. Additionally, the elevated expression of miR-21 provoked by LPS was further enhanced by AMPs. Conclusions: In conclusion, AMPs could alleviate LPS-induced cardiomyocytes cells injury via up-regulation of miR-21, miR-146, and suppression of TLR4/NF-jB pathway, which plays a key role in the down-regulation of LPS-mediated miR-155 and inflammatory cytokine expression.

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Comprehensive evaluation of ethanol-preserved amniotic extracts: Exploring antioxidant properties, proliferation enhancement, protective efficacy and regeneration potential in wound healing

Elkhenany,

Abou-Shanab,

Magdy

et al. 2024

Journal of Drug Delivery Science and Technology

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Topical application of lyophilized and powdered human amniotic membrane promotes diabetic ulcer healing

Cited by 4 publications

References 42 publications

Determining MMP‐2 and MMP‐9 reductive activities of bovine and equine amniotic membranes homogenates using fluorescence resonance energy transfer

Determining MMP‐2 and MMP‐9 reductive activities of bovine and equine amniotic membranes homogenates using fluorescence resonance energy transfer

Amnion membrane proteins attenuate LPS-induced inflammation and apoptosis by inhibiting TLR4/NF-κB pathway and repressing MicroRNA-155 in rat H9c2 cells

Comprehensive evaluation of ethanol-preserved amniotic extracts: Exploring antioxidant properties, proliferation enhancement, protective efficacy and regeneration potential in wound healing

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