Corneal healing involves a complex interaction of multiple proteinases, growth factors, and cytokines, which coordinate cell death, migration, proliferation, and differentiation, as well as extracellular matrix remodeling. 1 To maintain the corneal stroma's collagen and proteoglycan structure following ulceration, an equilibrium between

Alguns aspectos biométricos do aparelho genital externo de jumentos doadores de sêmen da raça PêgaSome biometric aspects of the external genital tract from donkey Pêga breed semen donors RESUMO Pêga, com peso médio de 272±34, de 7,4 (5,9 a 9,6), e o VT esquerdo foi de 155,5±14,4mL, enquanto o direito foi de 149,3±14,9. A medida de FUND foi de 25,3±3,3mm (20,3a 30,8mm) (TH -dorso ventral) and thickness of spermatic funiculum (FUN -third part). The biometric data from biometric testis were used to calculate the testis indices (TI) and testis volume (TV). The TI observed was 7.4 (5.9 a 9.6) and TV was 155.5±14.4, 149.3±14.9 respectively left and right. The average FUN was 25.3±3.3mm (20.3 a 30.8mm) O presente estudo foi conduzido com seis jumentos reprodutores da raça altura testicular (AT -dorso ventral), largura testicular (LT -médio lateral) e espessura do funículo (FUNterço médio). Os dados de biometria testicular foram utilizados para o cálculo do índice testicular (IT) e volume testicular (VT). O IT médio observado foi Palavras-chave: biometria, volume, índice testicular. ABSTRACT The present study was performed with six Pêga breed donkeys, weight from 231 to 326kg (272±34.9). The breeders were divided into the adult group (J1, J2 e J3, 15 years old average) and the young group (J4, J5 e J6, 3.5 years old average). . The donkeys presented normal rates of fertility

Objective To evaluate the efficacy of bovine amniotic membrane homogenate (BAMH) on wounded ex vivo rabbit corneas. Procedure Eighteen corneas obtained from normal rabbit eyes were wounded equally using a 6 mm trephine and cultured into an air‐liquid interface model. Corneas were treated with phosphate‐buffered saline (PBS) (n = 6, control group), 0.2% ethylenediaminetetraacetic acid (EDTA; n = 6), or BAMH (n = 6). All treatments were applied topically 6 times/day. Each cornea was macrophotographed daily with and without fluorescein stain to assess epithelialization and haziness. After 7 days, corneal transparency was evaluated, and the tissues prepared for histologic analysis of viability, and total and epithelial thickness. Results The mean epithelialization time was 6.2 ± 0.82 days for the control group, 6.2 ± 0.75 days for the EDTA‐treated group, and 5.1 ± 0.40 days for the BAMH‐treated group, demonstrating a significant difference between the BAMH and the other groups. The corneas that received EDTA had better transparency compared with the other groups. Histologically, all corneas had adequate morphology and architecture after healing. Analysis of corneal and epithelial thickness revealed no significant difference among groups. Conclusion Bovine amniotic membrane homogenate is an effective and promising treatment for stromal and epithelial ulcers.

Purpose To investigate the protein profile of bovine amniotic membranes (bAM) and to determine putative associations between protein composition in bAM and known corneal healing pathways. Methods The bAM were acquired from normal full-term births ( n = 10), processed, and stored at −80°C for two days. Subsequently, the frozen membranes were thawed at room temperature and prepared for proteomic exploration using high-resolution liquid chromatography–mass spectrometry, followed by bioinformatics analysis. Recently identified corneal healing pathways were contrasted with protein profiles and pathways present in bAM. Results The analyses identified 2105 proteins, with an interactive network of 1271 nodes (proteins) and 8757 edges (interactions). The proteins with higher betweenness centrality measurements include microfibril-associated protein 4, HSD3B1, CAPNS1, ATP1B3, CAV1, ANXA2, YARS, and GAPDH. The top four pathways in Kyoto Encyclopedia of Genes and Genomes were ribosome, metabolic pathway, spliceosome, and oxidative phosphorylation. The bAM and cornea shared abundant proteins, genome ontology, and signaling pathways. Conclusions The high-throughput proteomic profile of the bAM demonstrated that numerous proteins present in the cornea are also present in this fetal membrane. Our findings collectively demonstrate the similarity between bAM and the cornea's protein composition, supporting our hypothesis that bAM can be used to treat corneal diseases.

Efeitos da morfina e da metadona associadas à acepromazina em gatas anestesiadas com propofol e halotano e submetidas à ovariossalpingohisterectomiaEffects of morphine and methadone associated with acepromazine in cats anesthetized with propofol and halothane and subjected to ovariossalpingohisterectomia RESUMO Os fármacos utilizados na medicação pré-anestésica são úteis para preparar o paciente para anestesia, causando sedação, analgesia, redução na dose dos anestésicos e menor incidência de efeitos adversos ao paciente. O objetivo deste trabalho foi avaliar os efeitos cardiovasculares e respiratórios, comportamentais, relaxamento muscular, analgesia pós-operatória da morfina ou metadona associadas à acepromazina em gatas anestesiadas com propofol e halotano e submetidas a ovariossalpingohisterectomia. Para isso, foram utilizadas 20 gatas clinicamente sadias, distribuídas em 2 grupos: o grupo I recebeu a associação de acepromazina (0,05mg/kg) e metadona (0,2mg/kg) e o grupo II recebeu a associação de acepromazina (0,05mg/kg) e morfina (0,2mg/kg) administradas na mesma seringa por via intramuscular. Foram avaliados frequência cardíaca e respiratória, pressão arterial sistólica, saturação de oxi-hemoglobina, concentração de dióxido de carbono no ar expirado, temperatura retal e tempo de preenchimento capilar, nos períodos pré, trans e pós-operatórios. Pode-se concluir que ambas as associações produziram leve sedação e podem ser utilizadas com segurança em felinos, pois promoveram boa analgesia sem efeitos colaterais. ABSTRACTThe pre-anesthetic medication is useful to prepare the patient for anesthesia, causing sedation, analgesia, reduction of the anesthetic gas doses and a lower incidence of adverse effects to the patient. Such variation of effects is dependent on the combination of drugs used. The aim of this study was to evaluate the cardiovascular and respiratory effects, behavioral, muscle relaxation, postoperative analgesia and influence on the quality and recovery period of morphine or methadone. For that were used 20 clinically healthy cats, divided into 2 groups: group I received the combination of acepromazine (0.05mg/kg) and methadone (0.2mg/kg) and group II received a combination of acepromazine (0.05mg/kg) and morphine (0.2mg/kg) administered in the same syringe for intramuscular injection. It was concluded that both associations produced mild sedation and can be used safely in cats, therefore providing good analgesia and muscle relaxation, without side effects.