Determining MMP‐2 and MMP‐9 reductive activities of bovine and equine amniotic membranes homogenates using fluorescence resonance energy transfer

Abstract

Corneal healing involves a complex interaction of multiple proteinases, growth factors, and cytokines, which coordinate cell death, migration, proliferation, and differentiation, as well as extracellular matrix remodeling. 1 To maintain the corneal stroma's collagen and proteoglycan structure following ulceration, an equilibrium between

Effects of commercial amniotic membrane extract on the re‐epithelialization time and the early expression of matrix metalloproteinase‐9 in cats with experimentally induced corneal ulcers

“…To date, in most of the studies that investigated the effects of the AMEs, the eye drops were manufactured at the institutions responsible for developing the research 9,10,12–20,32 . In a clinical setting, the preparation and preservation of AME may be difficult or impracticable.…”

“…Other components, such as epidermal growth factor, hepatocyte growth factor, and fibroblast growth factor basic, which together promote the migration and proliferation of corneal epithelial cells, have also been found in AME 9–11 . Tissue inhibitors of metalloproteinase‐1, metalloproteinase‐2, and metalloproteinase‐3 were isolated from the AME of dogs, cows, and horses 7,12 . Additionally, a novel component known as heavy chain‐hyaluronan pentraxin 3, which is a matrix component with anti‐inflammatory, anti‐angiogenesis, and anti‐scarring effects, has been recognized and reported in the literature 11 .…”

Biological Compositions of Canine Amniotic Membrane and Its Extracts and the Investigation of Corneal Wound Healing Efficacy In Vitro

“…From our study, the TIMP-1 protein was identified in cAM similar to a recent study by Dower et al (2021) [ 30 ]. Moreover, the TIMP-1 protein was also detected in hAM [ 33 ] and bovine AM homogenate (bAMH) [ 34 ]. In the same study, the TIMP-1 protein was not detected in equine AM homogenate (eAMH) [ 34 ].…”

“…Moreover, the TIMP-1 protein was also detected in hAM [ 33 ] and bovine AM homogenate (bAMH) [ 34 ]. In the same study, the TIMP-1 protein was not detected in equine AM homogenate (eAMH) [ 34 ]. In the case of TIMP-2 expression, the presence of the TIMP-2 gene was identified in cAM similar to that in hAM [ 33 ].…”

“…In the case of TIMP-2 expression, the presence of the TIMP-2 gene was identified in cAM similar to that in hAM [ 33 ]. As for protein level, the expression of TIMP-2 was also detected in bAMH and eAMH [ 34 ]. A similar finding of TSP-1 at gene level in cAM was reported in hAM [ 33 ].…”