Titanium phosphate glass microspheres for bone tissue engineering

Abstract

We have demonstrated the successful production of titanium phosphate glass microspheres in the size range of ∼10-200 μm using an inexpensive, efficient, easily scalable process and assessed their use in bone tissue engineering applications. Glasses of the following compositions were prepared by melt-quench techniques: 0.5P₂O₅-0.4CaO-(0.1-x)Na₂O-xTiO₂, where x=0.03, 0.05 and 0.07 mol fraction (denoted as Ti3, Ti5 and Ti7 respectively). Several characterization studies such as differential thermal analysis, degradation (performed using a novel time lapse imaging technique) and pH and ion release measurements revealed significant densification of the glass structure with increased incorporation of TiO₂ in the glass from 3 to 5 mol.%, although further TiO₂ incorporation up to 7 mol.% did not affect the glass structure to the same extent. Cell culture studies performed using MG63 cells over a 7-day period clearly showed the ability of the microspheres to provide a stable surface for cell attachment, growth and proliferation. Taken together, the results confirm that 5 mol.% TiO₂ glass microspheres, on account of their relative ease of preparation and favourable biocompatibility, are worthy candidates for use as substrate materials in bone tissue engineering applications.

Structural characterization of titanium-doped Bioglass using isotopic substitution neutron diffraction

“…All 29 Si data were externally referenced to kaolinite (d = À92 ppm). Corresponding 31 P MAS NMR data were acquired at 9.4 T using a Bruker DSX-400 spectrometer operating at a characteristic 31 P frequency of 161.44 MHz. Single pulse experiments were implemented using a Bruker 3.2 mm double-air-bearing probe which enabled MAS frequencies of up to 20 kHz; p/4 pulse widths of 1.5 ms and recycle delays of 30 s were common to these measurements.…”

“…8,30 Cell culture studies have shown Ti phosphate microspheres provide a stable surface for cell attachment, growth and proliferation. 31 However, there have been no structural studies of melt quench derived Ti-doped bioactive silicate glasses.…”

Preparation and characterization of vanillin-crosslinked chitosan therapeutic bioactive microcarriers

“…Afterwards, each insert was taken out of the well and the entire insert mesh was carefully cut out from the insert along the edge using a scalpel. The samples were sputter-coated with gold before SEM observation [25].…”

In vitro bioactivity of titanium-doped bioglass

“…Titanium is also routinely incorporated into bioactive phosphate glasses to control the solubility and is reported to increase biocompatibility [13][14][15]. Cell culture studies have shown Ti phosphate microspheres provide a stable surface for cell attachment, growth and proliferation [16]. Wakamura et al have also shown that Ti incorporated into HA is bactericidal [17].…”