Titanium dioxide nanoparticles affect osteoblast-derived exosome cargos and impair osteogenic differentiation of human mesenchymal stem cells

Souza, Wanderson de; Gemini-Piperni, Sara; Grenho, Liliana; Rocha, L.A.; Granjeiro, José Mauro; Melo, Sónia A.; Fernandes, María Helena; Ribeiro, Ana R.

doi:10.1039/d2bm01854c

Cited by 8 publications

(3 citation statements)

References 94 publications

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“… 96 In contrast, EVs secreted by osteoblasts within the pathological microenvironment inhibited the osteogenic differentiation of MSCs. 97 , 98 Interestingly, there is a lack of consensus regarding the regulatory effects of osteoblast-derived EVs on osteoclast differentiation. Li et al demonstrated that osteoblast-derived exosomes enriched with miR-503-3p suppressed osteoclast differentiation by downregulating heparanase gene expression.…”

Section: The Potential Role Of Evs Derived From Different Sources In ...mentioning

confidence: 99%

The role and applications of extracellular vesicles in osteoporosis

Fang,

Yang,

Wang

et al. 2024

Bone Res

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Osteoporosis is a widely observed condition characterized by the systemic deterioration of bone mass and microarchitecture, which increases patient susceptibility to fragile fractures. The intricate mechanisms governing bone homeostasis are substantially impacted by extracellular vesicles (EVs), which play crucial roles in both pathological and physiological contexts. EVs derived from various sources exert distinct effects on osteoporosis. Specifically, EVs released by osteoblasts, endothelial cells, myocytes, and mesenchymal stem cells contribute to bone formation due to their unique cargo of proteins, miRNAs, and cytokines. Conversely, EVs secreted by osteoclasts and immune cells promote bone resorption and inhibit bone formation. Furthermore, the use of EVs as therapeutic modalities or biomaterials for diagnosing and managing osteoporosis is promising. Here, we review the current understanding of the impact of EVs on bone homeostasis, including the classification and biogenesis of EVs and the intricate regulatory mechanisms of EVs in osteoporosis. Furthermore, we present an overview of the latest research progress on diagnosing and treating osteoporosis by using EVs. Finally, we discuss the challenges and prospects of translational research on the use of EVs in osteoporosis.

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Section: The Potential Role Of Evs Derived From Different Sources In ...mentioning

confidence: 99%

The role and applications of extracellular vesicles in osteoporosis

Fang,

Yang,

Wang

et al. 2024

Bone Res

View full text Add to dashboard Cite

show abstract

“…Studies have shown that MSC‐derived exosomes (MSC‐Exos) can function well in different environments. MSC‐Exos induced repair in mouse models of wound healing and myocardial infarction 160–162 . EVs of MSCs are rich in MSC‐derived bioactive molecules and regulate the phenotype, function, and homing of immune cells.…”

Section: Stem Cell Exosomes In Regenerative Medicinementioning

confidence: 99%

“…MSC‐Exos induced repair in mouse models of wound healing and myocardial infarction. 160 , 161 , 162 EVs of MSCs are rich in MSC‐derived bioactive molecules and regulate the phenotype, function, and homing of immune cells. Application of MSC‐EVs has a significant inhibitory effect on the inflammatory response of an organism, accelerating the survival and regeneration of damaged parenchymal cells.…”

Section: Stem Cell Exosomes In Regenerative Medicinementioning

confidence: 99%

Application of stem cells in regeneration medicine

Jin

et al. 2023

MedComm

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Regeneration is a complex process affected by many elements independent or combined, including inflammation, proliferation, and tissue remodeling. Stem cells is a class of primitive cells with the potentiality of differentiation, regenerate with self‐replication, multidirectional differentiation, and immunomodulatory functions. Stem cells and their cytokines not only inextricably linked to the regeneration of ectodermal and skin tissues, but also can be used for the treatment of a variety of chronic wounds. Stem cells can produce exosomes in a paracrine manner. Stem cell exosomes play an important role in tissue regeneration, repair, and accelerated wound healing, the biological properties of which are similar with stem cells, while stem cell exosomes are safer and more effective. Skin and bone tissues are critical organs in the body, which are essential for sustaining life activities. The weak repairing ability leads a pronounced impact on the quality of life of patients, which could be alleviated by stem cell exosomes treatment. However, there are obstacles that stem cells and stem cells exosomes trough skin for improved bioavailability. This paper summarizes the applications and mechanisms of stem cells and stem cells exosomes for skin and bone healing. We also propose new ways of utilizing stem cells and their exosomes through different nanoformulations, liposomes and nanoliposomes, polymer micelles, microspheres, hydrogels, and scaffold microneedles, to improve their use in tissue healing and regeneration.

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In vivo assessment of TiO2 based wear nanoparticles in periprosthetic tissues

Gregar,

Gallo,

Milde

et al. 2024

Anal Bioanal Chem

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A multimodal approach combining inductively coupled plasma mass spectrometry (ICP-MS), single-particle ICP-MS (spICP-MS), scanning electron microscopy-energy-dispersive X-ray spectroscopy (SEM-EDS) and Raman spectroscopy enabled a deeper insight into the balance between total titanium (Ti), the soluble titanium fraction and titanium dioxide based particle fraction levels in periprosthetic tissues collected from patients undergoing revision surgery. Hydrofluoric acid usage in the sample digestion allowed for complete digestion of TiO2 particles, thus enabling accurate estimation of total Ti levels. The TiO2 fraction represents 38–94% of the titanium load in the six samples where particles were detected, and the fraction is present mainly in samples from patients with aseptically loosened total hip arthroplasty. Further attention was given to this fraction determining the elemental composition, particle count, particle size and modification of TiO2. The spICP-MS analysis confirmed the presence of the TiO2-derived (nano)particles (NPs) with a 39- to 187-nm median size and particle count up to 2.3 × 1011 particles per gram of tissue. On top of that, the SEM-EDS confirmed the presence of the TiO2 nanoparticles with 230-nm median size and an anatase crystal phase was determined by Raman spectroscopy. This study presents a novel multimodal approach for TiO2 particle determination and characterization in tissue samples and is the first in vivo study of this character.

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Titanium dioxide nanoparticles affect osteoblast-derived exosome cargos and impair osteogenic differentiation of human mesenchymal stem cells

Abstract: Titanium implants undergo tribocorrosion processes releasing particles that interact with several cells at the implant–bone interface. Osteoblasts-derived exosomal proteins reduce osteogenic differentiation of HMSCs contributing to joint failure.

Cited by 8 publications

References 94 publications

The role and applications of extracellular vesicles in osteoporosis

The role and applications of extracellular vesicles in osteoporosis

Application of stem cells in regeneration medicine

In vivo assessment of TiO2 based wear nanoparticles in periprosthetic tissues

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