Tissue distribution and elimination of florfenicol in topmouth culter (Culter alburnus) after oral administration

Wang, Weifen; Dai, Xianjun; Li, Zhiqiang; Meng, Qingkai

doi:10.17221/183/2008-cjfs

Cited by 10 publications

(6 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of the present study are also in line with Anadón et al , who determined FF residues in chicken using a validated HPLC technique. Furthermore, the present results are confirmed by various other studies that used an HPLC‐UV method to determine FF residues in chickens or broilers …”

Section: Discussionsupporting

confidence: 87%

See 1 more Smart Citation

Determination of florfenicol residues in broiler meat and liver samples using RP‐HPLC with UV–visible detection

et al. 2015

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 87%

“…Detection was done with the UV–visible detector set at 223 nm. The flow rate was maintained at 1 mL min −1 . Representative chromatograms of (a) FF standard, (b) residues in meat and (c) residues in liver are shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

Determination of florfenicol residues in broiler meat and liver samples using RP‐HPLC with UV–visible detection

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The flow rate was kept at 1.0 ml/min. The UV detector was adjusted at wavelength of 223nm (Wang et al, 2009). The representative chromatograms of standard, meat and egg are shown in Fig.…”

Section: Extraction Of Residuesmentioning

confidence: 99%

Estimation of Florfenicol Residues in Layer Meat and Egg Samples using High Performance Liquid Chromatography

Fahim¹

2018

PVJ

View full text Add to dashboard Cite

Antibiotics are widely used in the poultry industry to enhance the health and productivity of flocks which may have adverse effects on consumer's health. It is necessary to screen food products from animal origin for antimicrobial residues to safeguard the consumer's health. The present study was aimed to detect florfenicol (FF) residues in meat and egg samples of layer birds. For this purpose 150 meat and eggs samples were collected in equal ratio. High performance liquid chromatography (HPLC) was used to determine residual concentration of FF in meat and egg samples at wavelength of 223 nm. Ethyl acetate and phosphate buffer saline solution were used for extraction of FF from the samples. The mobile phase contained acetonitrile and water (27:73 v/v). Mean residual concentrations of FF as 61.56±13.19 and 281.08±57.46 µg/kg in meat and egg samples was detected. This study also showed that 80% (60) meat and 72% (54) egg samples were FF residue positive, out of these 86.7% (52) meat and 55.6% (30) egg samples were found to have residual concentrations above maximum residual limits. This contaminated meat may cause public health issues. There is a need to develop legislation about residual concentration of drugs in animal food products in Pakistan as well as to inform formers about the detrimental effects of drug residues on human health. To Cite This Article: FahimA, Aslam B, Mohsin M, Raza A, Faisal MN and Hussain A, 2018. Estimation of florfenicol residues in layer meat and egg samples using high performance liquid chromatography. Pak Vet J, 38(3): 329-332. http://dx.

show abstract

“…Extraction of the drug residues from the samples was carried out according to Wang et al [8]. Five grams of the ground sample were weighed in 40 mL centrifuge tube.…”

Section: Drug Residues Extractionmentioning

confidence: 99%

Effect of Florfenicol on Hematology, Cardiac Enzymes and its Residues in Broiler Chickens by HPLC

Said

El‐Nabtity

Selim³

et al. 2016

Zagazig Veterinary Journal

View full text Add to dashboard Cite

The current study was conducted on multiple oral doses (40 mg/kg for 3 successive days) of florfenicol (FF) to determine its effect on some hematological parameters, cardiac enzymes and its residues in blood and tissues (liver, muscles and kidneys) of broiler chickens. Seventy broiler chickens were used and divided into two groups, each consisted of 35 birds. The first group was left as a control, while the second was given FF for 3 successive days. The FF residues in tissues were determined using reversed phase-high performance liquid chromatography (RP-HPLC) with ultraviolet (UV) detector at 223 nm. Results indicated a widespread distribution of FF in most of the tested tissues. All tissue samples were considered FF free on the 9 th day after the last oral dose except liver. Florfenicol administration elicited a significant decrease in all blood parameters (hemoglobin concentration (Hb), red blood cell count (RBCs), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC) and lymphocytes) from 1 st up to 7 th day except packed cell volume (PCV) which showed no significant change on 1 st day but decreased on 3 rd , 5 th and 7 th day after stopping medication. Moreover, monocytopenia was observed on the 5 th and 7 th day and white blood cells (WBCs) showed the same effect on the 5 th day, while heterophiles revealed highly elevation. All cardiac enzymes (aspartate aminotransferase (AST), lactate dehydrogenase (LDH), creatine kinase MB (CK-MB) and Troponin I) were highly elevated. In conclusion, broiler chicken meat can be consumed safely after 9 days post FF treatment.

show abstract

Tissue distribution and elimination of florfenicol in topmouth culter (Culter alburnus) after oral administration

Cited by 10 publications

References 16 publications

Determination of florfenicol residues in broiler meat and liver samples using RP‐HPLC with UV–visible detection

Determination of florfenicol residues in broiler meat and liver samples using RP‐HPLC with UV–visible detection

Estimation of Florfenicol Residues in Layer Meat and Egg Samples using High Performance Liquid Chromatography

Effect of Florfenicol on Hematology, Cardiac Enzymes and its Residues in Broiler Chickens by HPLC

Contact Info

Product

Resources

About