Fatty acid profile data for refined cold-pressed Trichosanthes kirilowii Maxim. seed oil, in comparison to other commercially available oils — olive, rapeseed and camellia, are presented. Trichosanthes kirilowii Maxim. seed oil has high oleic and linoleic acid content and high polyunsaturated acid content. Squalene elutes as a distinct peak in the GC chromatograph. For a positive identification, MS detection was used. In the samples analyzed, squalene occurred in the range of 57.4–68.2 mg g−1.
Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide. However, there is no effective treatment for HCC. It has been shown that sustained activation of telomerase is essential for the growth and progression of HCC, suggesting that telomerase is a rational target for HCC therapy. Here, we investigated the effects of siRNA-mediated knockdown of hTERT, the catalytic and rate-limiting subunit of telomerase, on the sensitivity of HCC cells to cisplatin. While silencing of hTERT and the resultant inhibition of telomerase activity by infection with the recombinant adenovirus expressing a hTERT siRNA (Ad-si/hTERT) alone did not affect the proliferation and viability of SMMC7721 and HepG2 HCC cells within five days, co-administration of Ad-si/hTERT, but not the empty adenovirus vector, with cisplatin caused much greater extent of apoptosis in vitro under the same conditions and induced significantly more robust inhibition of SMMC7721 and HepG2 tumors growth in a mouse tumor xenograft model than cisplatin monotherapy. Our results demonstrated the synergistic effect between hTERT siRNA and cisplatin in the suppression of HCC progression and indicated that the combination of hTERT-specific siRNA and cisplatin could be an effective therapy for HCC.
Contamination of Cronobacter spp. (Enterobacter sakazakii) in infant formulas and other food products is a severe problem. Here a loop-mediated isothermal amplification (LAMP) assay was developed for rapidly detecting Cronobacter spp. in powdered infant formula. Sequences of 16S/23S rDNA internal intergenic spacer of Cronobacter spp. were used as the target template to design LAMP primers. The detection outcome can be evaluated by the white precipitate or the fluorescence intensity under ultraviolet irradiation, both visible to naked eyes. The sensitivity and specificity of the LAMP assay was further analyzed in comparison with that of regular PCR and real time quantitative PCR. The results showed that all of Cronobacter spp. strains display positive reaction to the detections while all of the non-Cronobacter spp. strains were negative, and that the LAMP assay exhibits a high sensitivity of 9.1 fg/μL (The sensitivity of regular PCR and real time quantitative PCR is 91 and 9.1 pg/μL, respectively.). The amplified reaction could be accomplished in about 1 h, with the results visible to naked eyes. Hence, the LAMP assay developed by this study can provide a rapid and simple approach for the detection of Cronobacter spp. in infant formula.
As an important chemokine receptor, the role of CCR4 in the progression of bladder cancer (BC) remains unknown. In this study, we have shown that CCR4 expression was upregulated in bladder carcinoma tissues compared with adjacent nontumor tissues. Kaplan-Meier survival analysis revealed that CCR4 expression was an independent prognostic risk factor in BC patients, and the addition of CCL17 induced CCR4 production and promoted migration and invasion of BC cells. In addition, CCR4 knockdown significantly attenuated the migratory and invasive capabilities of BC cells. Mechanistically, CCL17-CCR4 axis is involved in ERK1/2 signaling and could mediate the migration and invasion of BC cells by regulating MMP13 activation. This study suggests that CCR4 might represent a promising prognostic biomarker and a potential therapeutic option for BC.
The serum concentration and muscle residue of florfenicol following oral administration were evaluated in topmouth culter (<I>Culter alburnus</I>) under field conditions. Fishes were cultured in tanks and fed a commercial medicated diet containing 2000 mg/kg florfenicol in a single dose. The sampling was conducted at different intervals (0, 0.5, 1, 1.5, 2, 3, 4, 8, 10, 12, 16, 24, 36, 48, 72, 96, 120, 144, 168 h) after the cessation of medication. Drug analysis was carried out by HPLC. The maximum concentration (<I>C</I><sub>max</sub>) of 10.8 μg/g in serum and 4.2 μg/g in muscle were reached at the same time (<I>t</I><sub>max</sub>) of 8 hours. If the EU MRL value of 1.0 μg/g is quoted for the topmouth culter tissue, the extrapolation of the data indicated that it will be passed after a 40.6 h withdrawal period for florfenicol in topmouth culter muscle.
Wang W., Zhang M., Fang J., Zhang L., Zou X., Wang X. (2013): Improved detection of Ochratoxin A by marine bioluminescent bacteria V. harveyi BA. Czech J. Food Sci., 31: 88-93.We applicate the bioluminescent assay system for evaluating the toxicity of Ochratoxin A (OTA). The optimum conditions for the growth and bioluminescence of V. harveyi BA were investigated, including NaCl concentration and pH in the medium, incubation temperature, and OTA action time. The growth and luminescence reached the perfect phase with the NaCl concentration in the range of 1% to 2%, pH 8-9, incubation temperature 25-30°C, and OTA acting for1 hour. Based on these optimum conditions for bioluminescence, the inhibitory effect of OTA on luminosity was pursued. When OTA concentration fell into the range of 0.1-1.0 µg/l, bioluminescence inhibition followed a linear pattern with a good correlation coefficient (R 2 = 0.944). The calculated recovery percentages fell into the range of 81-102% within the spiking range of 20-200 µg/kg. This system provided a screening method for the measurement of toxic OTA by monitoring the changes in luminescence.
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